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作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1942-1947.doi: 10.3724/SP.J.1006.2009.01942

• 研究简报 • 上一篇    

大豆脂肪酸合成关键酶基因的电子定位及结构分析

宋万坤1,2，朱命喜2，赵阳林1，王晶2，李文福2，刘春燕1,2，陈庆山2,*，胡国华1,3,*   

  1. 1黑龙江省农垦科研育种中心，黑龙江哈尔滨150090；2东北农业大学农学院，黑龙江哈尔滨150030；3高家大豆工程技术研究中心，黑龙江哈尔滨150050
  • 收稿日期:2009-01-15 修回日期:2009-04-26 出版日期:2009-10-12 网络出版日期:2009-07-04
  • 通讯作者: 胡国华, E-mail: hugh757@vip.163.com; 陈庆山, E-mail: qshchen@126.com; Tel: 0451-55191945
  • 基金资助:

    本研究由农业部引进国际先进农业科学技术计划（948计划）项目【2006-G1（A）】，国家高技术研究发展计划（863计划）项目（2006AA10Z1F4），黑龙江省博士后科研启动基金（LHK-04014）资助。

In silico Mapping and Structure Analysis of Key Enzyme Genesis in Fatty Acid Synthesis of Soybean

SONG Wan-Kun1,2,ZHU Ming-Xi1,2,ZHAO Yang-Lin1,WANG Jing1,LI Wen-Fu1,LIU Chun-Yan1,2,CHEN Qing-Shan2,*,HU Guo-Hua1,3,*   

  1. 1The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090,China;2Agriculture College,Northeast Agricultural University,Harbin 150030,China;3The National Research Center of Soybean Engineering and Technology,Harbin 150050,China
  • Received:2009-01-15 Revised:2009-04-26 Published:2009-10-12 Published online:2009-07-04
  • Contact: HU Guo-hua,E-mail:hugh757@vip.163.com;CHEN Qing-Shan,E-mail:qshchen@126.com;Tel:0451-55191945

PDF

2419

被引次数

4

摘要：

利用最新大豆遗传图与物理图的整合图谱，对12个大豆脂肪酸合成中乙酰辅酶A羧化酶、脂肪酸合酶等关键酶基因进行基因定位与结构分析。它们分别被定位在A2、B2、C2、D1b、D2、G、I、L、M等9个连锁群上，并通过序列比对获得了所在相应连锁群区间两侧标记。同时，比较基因的cDNA和gDNA序列信息，获得了这12个基因结构信息，内含子数目从最少的0到最多的30个，其中FAD2-1、FAD2-2、FAD2-3与FatB是单外显子基因，没有内含子；其余基因都有内含子，KASI有6个，KASII有12个，SACPD有2个，FAD3有7个。通过定位获得对应分子标记可以为基因的分子辅助育种提供参考资料，而基因结构信息能更好地用于基因的功能分析。

关键词: 大豆, 脂肪酸, 基因, 电子定位, 基因结构

Abstract:

The 12 genes of key enzyme such as acyl-CoA carboxylase and fatty acid synthase in fatty acid synthesis were mapped on the newest genetic map integrating from physical map and genetic map, and the gene structure was analyzed. They were mapped on nine linkage groups, including A2, B2, C2, D1b, D2, G, I, L, M, and the flank markers of the gene on the linkage groups were obtained. At the same time, we compared the sequence information of cDNA with gDNA to get the structure information of the 12 genes, with the number of intron from 0 to 30. FAD2-1, FAD2-2, FAD2-3, and FatB were all single extron gene, but there were six introns in KASI, twelve introns in KASII, two introns in SACPD, and seven introns in FAD3. So the corresponding markers obtained from the mapping are available for molecular assisted selection, while the structure information can be better used in gene function analysis.

Key words: Soybean, Fatty acid, Gene, In silico mapping, Gene structure

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