欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1936-1941.doi: 10.3724/SP.J.1006.2009.01936

• 研究简报 • 上一篇    下一篇

应用SRAP标记分析黑芝麻核心种质遗传多样性

车卓1,2，张艳欣2,**，孙建2，张秀荣2,*，尚勋武1，王化俊1   

  1. 1甘肃农业大学/甘肃省作物遗传改良与种质创新重点实验室，甘肃兰州730070；2中国农业科学院油料作物研究所，湖北武汉430062
  • 收稿日期:2009-01-14 修回日期:2009-04-23 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 张秀荣，E-mail: zhangxr@oilcrops.cn
  • 基金资助:

    本研究由国家科技基础条件平台项目（2005DKA21001-20），国家科技支撑计划项目（2006BAD13B05-2），农业部物质保护项目（NB08-2130135-34,35）资助。

Genetic Diversity Analysis of Black Sesame(Sesamum indicum DC) Core Collection of China Using SRAP Markers

CHE Zhuo1,2,ZHANG Yan-Xin2,**,SUN Jian2,ZHANG Xiu-Rong2,*,SHANG Xun-Wu1,WANG Hua-Jun1   

  1. 1 Gansu Agricultural University / Gansu Key Laboratory of Crop Genetic Improvement and Germplasm Enhancement, Lanzhou 730070, China; 2 Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Received:2009-01-14 Revised:2009-04-23 Published:2009-10-12 Published online:2009-08-07
  • Contact: ZHANG Xiu-Rong，E-mail: zhangxr@oilcrops.cn

PDF

2141

被引次数

32

摘要：

利用SRAP分子标记技术对中国芝麻资源核心收集品中的黑芝麻种质进行遗传多样性分析。结果表明，13对引物组合对100份黑芝麻核心种质共扩增出稳定清晰的条带182条，其中多态性条带126条，占69.2%，每对引物组合的条带数和多态性带数分别为14.0个和9.7个；供试材料间成对遗传相似系数介于0.469~0.986，平均为0.726，通过UPGMA法，在遗传相似系数为0.68处可将供试材料聚为5个类群，表明黑芝麻核心种质具有较丰富的遗传多样性，聚类结果与地理分布没有明显的关系；遗传多样性指数是南方黑芝麻核心种质(0.3557)＞中部种质(0.3415)＞北方种质(0.2986)。本研究结果较全面反映了中国保存的黑芝麻种质资源遗传多样性特点，为我国黑芝麻资源进一步考察收集和引进，以及优异黑芝麻基因资源挖掘和育种利用提供了理论依据。

关键词: 黑芝麻, 核心种质, SRAP, 遗传多样性

Abstract:

China has a long history and rich germplasm resources for growing black sesame. Black sesame is well used because of its nutrition superior to black rice and black soybean. However, Research on black sesame is relatively less than that on other crops. In the paper, genetic diversity of black sesame core collection of China was analyzed using SRAP (sequence-related amplified polymorphism) markers. The results showed that 13 SRAP primer combinations were employed to evaluate the genetic diversity of 100 black sesame accessions, a total of 182 amplified fragments were detected and 126 of them were polymorphic, the polymorphism percentage was 69.2%, the number of amplified fragments and polymorphic fragments of each primer combination were 14.0 and 9.7, respectively. The 100 accessions were grouped into five clusters at genetic similarity of 0.68, indicating the genetic diversity of these accessions was abundant. As regards geographic regions, Shannon's information index of black sesame accessions in south China (0.3557) was the highest，the followings were that in central regions of China (0.3415) and north China (0.2986). The characteristics of genetic diversity of black sesame core collection of China were fully revealed in this study, which provided the theoretical foundations for further exploring, collecting and introducing black sesame germplasm as well as mining and utilizing excellent black sesame germplasm in the future.

Key words: Black sesame, Core collection, SRAP, Genetic diversity

[1] Shen M(沈梅). Measurements oil trace elements of black sesame and white sesame. Chin J Health Lab Tech (中国卫生检验杂志), 2007, 17(2): 2309-2310 (in Chinese with English abstract)

[2] Chen H-X(陈和兴), Liu F-L(刘凤兰), Zhao Y-Z(赵应忠). Breeding for Zhongzhi 9, a black seed coat sesame cultivar with high quality. Oil Crop Chin (中国油料), 1994, 16(4): 53-58 (in Chinese with English abstract)

[3] Xiao T-X(肖唐华), Feng X-Y(冯祥运), Zhang X-R(张秀荣). Analysis of the distribution and main characteristics of black seed coat sesame germplasm in china. Oil Crop Chin (中国油料), 1992, 14(2): 31-34 (in Chinese with English abstract)

[4] Le M-W(乐美旺), Zhang D-X(张冬仙), Xiong Y-P(熊永鹏), Zhou H-Y(周红英). Principal component analysis on quantitative characters and its application in black sesame. Oil Crop Chin (中国油料), 1995, 17(3): 72-76 (in Chinese with English abstract)

[5] Bhat K V, Babrekar P P, Lakhanpaul S. Study of genetic diversity in Indian and exotic sesame (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers. Euphytica, 1999, 110: 21-33

[6] Kim D H, Zur G, Danin P Y, Lee S W, Shim K B, Kang C W, Kashi Y. Genetic relationships of sesame germplasm collection as revealed by inter-simple sequence repeats. Plant Breed, 2002, 121: 259-262

[7] Ercan A G, Taskin M, Turgut K. Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genet Resour Crop Evol, 2004, 51: 599-607

[8] Laurentin H E, Karlovsky P. Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). BMC Genetics,2006, 7: 10

[9] Laurentin H E, Karlovsky P. AFLP fingerprinting of sesame (Sesamum indicum L.) cultivars: Identification, genetic relationship and comparison of AFLP informativeness parameters. Genet Resour Crop Evol, 2007, 54: 1437-1446

[10] Laurentin H E, Karlovsky P. Relationship between metabolic and genomic diversity in sesame (Sesamum indicum L.). BMC Genomics, 2008, 9: 250

[11] Zhang X-R(张秀荣), Chen K-R(陈坤荣), Peng J(彭俊), Xu Z-Y(许泽永). The RAPD analysis and genetic diversity of selected sesame germplasms. Chin J Oil Crop Sci (中国油料作物学报), 2004, 26(4): 34-37 (in Chinese with English abstract)

[12] Zhang P(张鹏), Zhang H-Y(张海洋), Guo W-Z(郭旺珍), Zheng Y-Z(郑永战), Wei L-B(魏利斌), Zhang T-Z(张天真). Genetic diversity analysis of Sesamum indicum L. germplasms using SRAP and EST-SSR markers. Acta Agron Sin (作物学报), 2007, 33(10): 1696-1702 (in Chinese with English abstract)

[13] Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103: 455-461

[14] Zhang X R, Zhao Y Z, Cheng Y, Feng X Y, Guo Q Y, Zhou M D, Toby Hodgkin. Establishment of sesame germplasm core collection in China. Genet Resour Crop Evol, 2000, 47: 273-279

[15] Doyle J J, Doyle J L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull, 1987, 19: 11-15

[16] Che Z(车卓), Zhang Y-X(张艳欣), Sun J(孙建), Huang B(黄波), Zhang X-R(张秀荣). Establishment and optimization of SRAP reaction system in sesame. Chin Agric Sci Bull (中国农学通报), 2008, 24(10): 74-77 (in Chinese with English abstract)

[17] Bassam B, Caetano-Anolles G, Gresshoff P M. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem, 1991, 196: 80-83

[18] Chen C-Y(陈翠云). Sesame Cultivars Resources in China (中国芝麻品种志), Beijing: Agriculture Press, 1990. pp 5-33 (in Chinese)

[19] Oil Crop Research Institute of CAAS (中国农业科学院油料作物研究所). The Catalogue of Varietal Germplasm Resources of sesame in China (中国芝麻品种资源目录), Vol 3. Beijing: China Agricultural Scientech Press, 1997 (in Chinese)

[20] Wen Y-C(文雁成), Wang H-Z(王汉中), Shen J-X(沈金雄), Liu G-H(刘贵华), Zhang X-F(张书芬). Analysis of genetic diversity and genetic basis of Chinese rapeseed cultivars (Brassica napus L.) by sequence-related amplified polymorphism markers. Sci Agric Sin (中国农业科学), 2006, 39(2): 246-256 (in Chinese with English abstract)

[21] Budak H, Shearman R C, Parmaksiz I, Gaussoin R E, Riordan T P, Dweikat I. Molecular characterization of buffalo grass germplasm using sequence-related amplified polymorphism markers. Theor Appl Genet, 2004, 108: 328-334

[22] Ferriol M, Pioó B, Nuez E. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor Appl Genet, 2003, 107: 271-282

[23] Wang H-Z(王华忠), Wu Z-D(吴则东), Wang X-W(王晓武), Fang Z-Y(方智远). Analysis of the genetic diversity in different types of sugar beets by SRAP and SSR markers. Acta Agron Sin (作物学报), 2008, 34(1): 37-46 (in Chinese with English abstract)

[24] Li W(李武), Ni W(倪薇), Lin Z-X(林忠旭), Zhang X-L(张献龙). Genetic diversity analysis of sea-island cotton cultivars using SRAP markers. Acta Agron Sin (作物学报), 2008, 34(5): 893-898 (in Chinese with English abstract)

[25] Jin W-L(金文林), Wen Z-X(文自翔), Pu S-J(濮绍京), Zhao B(赵波). Genetic diversity and evolution of Adzuki bean (Vigna angularis) germplasm resources based on RAPD markers. Sci Agric Sin (中国农业科学), 2005, 38(2): 241-249 (in Chinese with English abstract)

[26] Chen H(陈辉), Fan Y-H(范源洪), Shi X-W(史宪伟), Cai Q(蔡青), Zhang M(张明), Zhang Y-P(张亚平). Research on genetic diversity and systemic evolution in Saccharum spontaneum L. Acta Agron Sin (作物学报), 2001, 27(5): 645-652 (in Chinese with English abstract)
[27] Gai J-Y(盖钧镒), Xu D-H(许东河), Gao Z(高忠), Yoshiya S(岛本义也), Jun A(阿部纯), Hirofumi F(福士泰史), Shunji K(北岛俊二). Studies on the evolutionary relationship among Eco-types of G. max and G. soja in China. Acta Agron Sin (作物学报), 2000, 26(5): 513-520 (in Chinese with English abstract)
[1] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[2] 徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 王若若, 王磊, 张立异. 397份高粱种质资源在贵州表型多样性分析及综合评价[J]. 作物学报, 2026, 52(4): 1073-1087.
[3] 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[4] 侯洁, 付朵朵, 武海峰, 郝宇琼, 郑兴卫, 武棒棒, 周凯, 李晓华, 郑军, 赵佳佳. 山西省小麦地方品种的染色体多样性及遗传效应分析[J]. 作物学报, 2026, 52(3): 746-763.
[5] 梅飘, 刘丁丁, 叶圆圆, 张晨禹, 丁诗琦, 李亚奇, 王培鑫, 梅菊芬, 马春雷. 基于茶树液相功能芯片的白化茶树资源遗传多样性分析[J]. 作物学报, 2025, 51(9): 2358-2370.
[6] 王天译, 杨绣娟, 赵佳佳, 郝宇琼, 郑兴卫, 武棒棒, 李晓华, 郝水源, 郑军. 山西小麦醇溶蛋白多样性及其对面粉品质效应研究[J]. 作物学报, 2025, 51(7): 1784-1800.
[7] 梁红凯, 赵苏蒙, 陆琼, 周鹏, 智慧, 刁现民, 贺强. 谷子微核心种质的构建[J]. 作物学报, 2025, 51(6): 1435-1444.
[8] 王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885.
[9] 张红岩, 敏玉霞, 滕长才, 彭小星, 陈志凯, 周仙莉, 娄树宝, 刘玉皎. 利用130K液相芯片分析中国蚕豆种质资源遗传多样性[J]. 作物学报, 2024, 50(8): 1989-2000.
[10] 李长喜, 董占鹏, 关永虎, 刘金伟, 李航, 梅拥军. 南疆陆地棉农艺性状与皮棉产量性状的遗传贡献及决策系数分析[J]. 作物学报, 2024, 50(6): 1486-1502.
[11] 丁艺冰, 辛旭霞, 冯智尊, 曹越, 郭娟, Dipak K SANTRA, 王瑞云, 陈喜明. 东北春播区糜子核心种质及其DNA分子身份证构建[J]. 作物学报, 2024, 50(5): 1181-1192.
[12] 柯会锋, 苏红梅, 孙正文, 谷淇深, 杨君, 王国宁, 徐东永, 王洪这, 吴立强, 张艳, 张桂寅, 马峙英, 王省芬. 棉花现代品种资源产量与纤维品质性状鉴定及分子标记评价[J]. 作物学报, 2024, 50(2): 280-293.
[13] 苏一钧, 赵路宽, 唐芬, 戴习彬, 孙亚伟, 周志林, 刘亚菊, 曹清河. 378份甘薯引进种遗传多样性及群体结构分析[J]. 作物学报, 2023, 49(9): 2582-2593.
[14] 王倩, 张立媛, 许月, 李海, 刘少雄, 薛亚鹏, 陆平, 王瑞云, 刘敏轩. 黍稷高基元EST-SSR标记开发及200份核心种质资源遗传多样性分析[J]. 作物学报, 2023, 49(8): 2308-2318.
[15] 卢茂昂, 彭小爱, 张玲, 汪建来, 何贤芳, 朱玉磊. 基于55K SNP芯片揭示小麦育种亲本遗传多样性[J]. 作物学报, 2023, 49(6): 1708-1714.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2