作物学报 ›› 2024, Vol. 50 ›› Issue (8): 1989-2000.doi: 10.3724/SP.J.1006.2024.34059
张红岩1,2,3(), 敏玉霞4(
), 滕长才1,2,3, 彭小星1,2,3, 陈志凯1,2,3, 周仙莉1,2,3, 娄树宝1,2,3,5, 刘玉皎2,3,*(
)
ZHANG Hong-Yan1,2,3(), MIN Yu-Xia4(
), TENG Chang-Cai1,2,3, PENG Xiao-Xing1,2,3, CHEN Zhi-Kai1,2,3, ZHOU Xian-Li1,2,3, LOU Shu-Bao1,2,3,5, LIU Yu-Jiao2,3,*(
)
摘要:
全面了解我国蚕豆种质资源遗传多样性和群体结构, 对优异资源的挖掘和创新利用具有重大意义。本研究利用130K液相芯片对822份中国蚕豆种质资源进行基因分型, 开展遗传多样性研究。经过严格过滤, 获得了30,946个高质量SNP位点, 其中多态性信息量(PIC)变化范围为0.0905~0.3750, 平均为0.2600; Nei’s基因多样性指数变化范围为0.0950~0.5000, 平均为0.3222。基于地理来源, 利用Nei’s基因多样性指数和PIC评价蚕豆资源遗传多样性, 发现江苏和四川蚕豆资源的遗传多样性较为丰富; 新疆蚕豆资源遗传多样性最低。群体结构分析表明, 822份蚕豆资源可被划分为2个亚群, 当Q≥0.6时, 717份资源遗传背景相对比较单一; 同一亚群内包含不同生态类型的材料, 表明材料间发生了基因交流或渐渗现象。基于邻接法(NJ)的聚类分析和主成分分析结果表明, 822份蚕豆资源同样分为2个类群, 与群体结构分析结果基本一致。总体而言, 地理来源邻近和生境相近的资源间的遗传关系较近, 且常被归为同一类群或亚群。分子方差分析结果表明, 个体间的遗传变异是总遗传变异的主要来源。
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