作物学报 ›› 2023, Vol. 49 ›› Issue (8): 2308-2318.doi: 10.3724/SP.J.1006.2023.24201
• 研究简报 • 上一篇
王倩1,2(), 张立媛3, 许月4, 李海5, 刘少雄2, 薛亚鹏1,2, 陆平2, 王瑞云1,*(), 刘敏轩2,*()
WANG Qian1,2(), ZHANG Li-Yuan3, XU Yue4, LI Hai5, LIU Shao-Xiong2, XUE Ya-Peng1,2, LU Ping2, WANG Rui-Yun1,*(), LIU Min-Xuan2,*()
摘要:
为搞清黍稷核心种质资源的遗传背景, 开发新的高基元EST-SSR标记用以评估国内不同生态区资源的遗传多样性, 为加快黍稷育种进程和挖掘优异种质提供依据。本研究基于转录组测序开发了200个高基元EST-SSR标记并进行多态性筛选, 利用筛选到的多态性标记评价200份黍稷核心种质资源的遗传多样性。结果表明, 200个标记在6份地理来源差异显著的黍稷材料中有52个呈多态性, 开发效率为26%, 其中四、五和六碱基重复多态性标记分别为17个(32.7%)、17个(32.7%)和18个(34.6%)。利用52个高基元EST-SSR多态性标记对200份核心资源进行检测, 共得到129个观测等位变异, 每个位点检测到2~3个; Shannon多样性指数为0.3093~1.0910, 平均为0.7277; 多态性信息含量为0.1948~0.8211, 平均为0.5104。基于UPGMA将200份资源划分为6个群组。基于Structure的遗传结构(K=7)将资源划分为7个类群, 黍稷资源主要来自4个(东北地区、华北地区、黄土高原和北方地区)基因库。基于主成分分析将材料聚为6个类群, 与其地理来源一致。构建了52个四-六碱基重复EST-SSR标记, 开发率为26%; 用其分析材料的遗传差异发现黍稷地方核心种质遗传多样性较为丰富。
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