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作物学报 ›› 2010, Vol. 36 ›› Issue (05): 833-839.doi: 10.3724/SP.J.1006.2010.00833

• 耕作栽培·生理生化 • 上一篇    下一篇

胞内钙库对小麦叶锈菌侵染之过敏反应的影响

张蓓,阎爱华,刘刚,刘猛,侯春燕,王冬梅*   

  1. 河北农业大学生命科学学院,河北保定071001
  • 收稿日期:2009-11-09 修回日期:2010-02-07 出版日期:2010-05-12 网络出版日期:2010-03-15
  • 通讯作者: 王冬梅, E-mail: dongmeiwang63@hotmail.com
  • 基金资助:

    本研究由国家自然科学基金(30671244),河北省应用基础研究计划重点基础研究项目(08965505D),河北省自然科学基金(303180,C2005000220,C2007000515)资助.

Effect of Intracellular Calcium Stores on Hypersensitive Reaction Induced by Wheat Leaf Rust Fungus

ZHANG Bei,YAN Ai-Hua,LIU Gang,LIU Meng,HOU Chun-Yan,WANG Dong-Mei*   

  1. College of Life Science,Agricultural University of Hebei,Baoding 071001,China
  • Received:2009-11-09 Revised:2010-02-07 Published:2010-05-12 Published online:2010-03-15
  • Contact: WANG Dong-Mei,E-mail:dongmeiwang63@hotmail.com

PDF

2083

被引次数

36

摘要：

使用影响胞内Ca2+库和钙通道的药物预注射小麦叶片，观察其对小麦受叶锈菌侵染诱发的过敏反应(HR)的影响。结果表明，对小麦叶片预注射不同浓度的胞内Ca2+螯合剂(BAPTA-AM)后接种叶锈菌小种260，随着注射药物浓度的增高，寄主细胞发生HR的面积逐渐减小。而注射胞内Ca2+激活剂(caffiene)后接种，HR的面积有所增加。进一步用胞内Ca2+通道抑制剂(herapin、RR和8-Br-cADPR)预处理，结果herapin对HR的影响呈浓度依赖型，而RR和8-Br-cADPR对HR没有明显作用。据此提出，胞内Ca2+可能参与小麦抵抗叶锈菌侵染过程中钙信号的形成，且这一过程主要通过IP3途径完成。

关键词: 小麦, 叶锈菌, Ca2+, 过敏性反应

Abstract:

With injecting drugs to affect intracellular calcium stores and calcium channel in Wheat (Triticum aestivum) leaves, we investigated hypersensitive reaction (HR) changes in wheat leaves invaded by leaf rust (Puccinia triticina). The results showed that the intracellular calcium chelators (BAPTA-AM) could obviously reduce the area of dying cells caused by HR. The higher the concentration was, the smaller the area of dying cell was. While intracellular calcium activator (caffeine) was injected into wheat leaves, HR could also be detected. The HR area increased with caffeine concentration. Calcium channel blockers (heparin, RR, and 8-Br-cADPR) were separately injected into wheat leaves before inoculation. The results showed that there was a concentration-depended effect on HR with herapin, and no obviously effect with RR and 8-Br-cADPR. Therefore, we deduced that intracellular calcium might involve in the formation of calcium signal transduction through IP3 pathway during infection of wheat leaf rust fungus.

Key words: Wheat(Triticum aestivum L.), Puccinia triticina, Calcium, Hypersensitive response



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