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作物学报 ›› 2010, Vol. 36 ›› Issue (07): 1135-1143.doi: 10.3724/SP.J.1006.2010.01135

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

番茄果实成熟相关基因SlPEL和SlAPL的表达特性

肖东1,2,**,肖阳3,**,蔡应繁4,邓小峰1,5,吴锁伟1,郑旭1,3,李晚忱5,吴翠平1,2,费章君6,牛应泽2,*,杨建平1,4,*   

  1. 1中国农业科学院作物科学研究所，北京100081；2四川农业大学农学院，四川雅安625014；3中国农业科学院研究生院，北京100081；4重庆邮电大学生物信息学院，重庆400065；5四川农业大学玉米研究所，四川雅安625014；6Boyce Thompson Institute for Plant Research,Comell University,Ithaca 14856,NY,USA
  • 收稿日期:2009-11-26 修回日期:2010-03-16 出版日期:2010-07-12 网络出版日期:2010-04-14
  • 通讯作者: 杨建平, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859; 牛应泽, E-mail: nyz@sicau.edu, Tel: 13981617895
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2008ZX08010-002), 国家高技术研究发展计划(863计划)项目(2008AA10Z121), 重庆市自然科学基金(CSTC2009BA1088), 中央级公益性科研院所基本科研业务费专项(2060302-2), 教育部留学回国人员启动费和农业部回国人员择优资助项目资助。

Isolation and Expression of Two Fruit Ripening Related Genes SlPEL and SlAPL in Tomato (Solanum lycopersicum)

XIAO Dong1,2, XIAO Yang3,**, CAI Ying-Fan4, DENG Xiao-Feng1,5, WU Suo-Wei1, ZHENG Xu1,3, LI Wan-Chen5, WU Cui-Ping1,2, FEI Zhang-Jun6, NIU Ying-Ze2,*,YANGJian-Ping1,4,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 College of Agronomy, Sichuan Agricultural University, Sichuan, Ya’an 625014, China; 3 Graduate School, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; 4 College of Bio-information, Chongqing University of Posts and Tele-communication, Chongqing 400065, China; 5 Institute of Maize Research, Sichuan Agricultural University, Sichuan, Ya’an 625014, China; 6 Boyce Thompson Institute for Plant Research, Cornell University, Ithaca 14853, NY, USA
  • Received:2009-11-26 Revised:2010-03-16 Published:2010-07-12 Published online:2010-04-14
  • Contact: YANG JIan-Ping, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859;NIU Ying-Ze, E-mail: nyz@sicau.edu, Tel: 13981617895

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被引次数

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摘要： 分析27个代表番茄不同发育阶段和生物反应的组织特异性、含有152 635个独立EST数据库的数码表达，发现果胶裂解酶基因 (pectate lyase, SlPEL) 和番茄AP2 Like (SlAPL)的转录受果实成熟的调节。以授粉后不同发育时期的番茄(品种为美味樱桃)果实为试材, 用半定量PCR和荧光实时定量PCR分析SlPEL的表达模式，结果表明，授粉后12 d，其表达水平明显上升；授粉后16~18 d，达到第一个小高峰；28 d到最高峰；从28 d到完全成熟逐步下降到第一个小高峰的水平。SlAPL的表达模式与SlPEL类似，但其表达启动的时期迟于SlPEL。从授粉后25 d，SlAPL转录启动；授粉后28~32 d，其转录水平上升到第一个小高峰；39 d达到最高峰，以后到完全成熟略有下降。该研究也印证利用EST的数据库进行基因数码表达分析的可行性。

关键词: 番茄, 果胶裂解酶基因, 番茄APETALA2 Like基因, 表达分析

Abstract: During ripening, fruits undergo complicated developmental changes with varying physiological and biochemical activities that affect color, flavor, and texture. In tomato, these changes include accumulation of ethylene, the red pigment lycopene and the cell wall hydrolase, polygalacturonase (PG). Recently, genes of PG, pectin esterase (PE), ACC synthetase, ACC oxidase, and ethylene syshetase etc. from tomato, banana, carica papaya, peach and mango have been cloned. In this report, a large tomato expressed sequence tag (EST) dataset (152 635 in total) from 27 different EST libraries was analyzed to gain insights into digital gene expressions of two fruit ripening related genes SlPEL and SlAPL in tomato. Their expression profiles were detected using semi-quantitative RT-PCR assays and real-time RT-PCR assays in tomato (cv. Meiweiyingtao) during fruit development and ripening. SlPEL expression increased in the fruit at 12 days after pollination, and reached the small peak 4-6 days later and the larger peak at 28 days after pollination; then dropped to the level of the small peak until red ripening stage. As SlPEL, SlAPL expression was also induced by the fruit ripening. But SlAPL expressed two weeks later than SlPEL. SlPEL expression achieved the small peak at 28-32 days after pollination and the large peak at 39 days after pollination; and kept the higher levels until red ripening stage. The results confirmed that the digital gene expression using different EST libraries is an effective way to check the research value genes.

Key words: Solanum lycopersicum, Pectate lyase, APETALA 2 Like, Expression Analysis

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