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作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1362-1370.doi: 10.3724/SP.J.1006.2010.01362

• 耕作栽培·生理生化 • 上一篇    下一篇

氮素对高大气CO2浓度下小麦叶片光合功能的影响

张绪成1,2,张福锁2,于显枫1,陈新平2   

  1. 1 甘肃省农业科学院 / 农业部西北作物抗旱栽培与耕作重点开放实验室，甘肃兰州 730070; 2 中国农业大学资源环境学院，北京 100193
  • 收稿日期:2010-01-18 修回日期:2010-04-17 出版日期:2010-08-12 网络出版日期:2010-06-11
  • 基金资助:
     本研究由国家自然科学基金项目(30800668)资助。

Effects of Nitrogen Nutrition on Photosynthetic Functions of Wheat Leaves under Elevated Atmospheric CO2 Concentration

ZHANG Xu-Cheng1,2, ZHANG Fu-Suo2, YU Xian-Feng1, and CHEN Xin-Ping2   

  1. 1 Key Laboratory of Northwest Crop Drought-resistant Farming, Ministry of Agriculture / Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 2 College of Resources and Environment, China Agricultural University, Beijing 100193, China
  • Received:2010-01-18 Revised:2010-04-17 Published:2010-08-12 Published online:2010-06-11

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被引次数

13

摘要： 为探讨高大气CO2浓度下植物光合作用适应现象的光合能量转化和分配的氮素响应及其对C3植物光合功能的影响，本试验对盆栽小麦进行2个大气CO2浓度和2个氮水平的组合处理，通过测定小麦光合气体交换参数、叶绿素荧光参数和叶绿素含量等指标，研究施氮对高大气CO2浓度下小麦叶片光合功能的影响。结果表明，大气CO2浓度升高后，低氮处理小麦叶片光合速率发生明显的适应性下调，光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)下降；但高氮叶片则无明显的光合作用适应现象发生。高大气CO2浓度下低氮叶片光化学速率、PSII线性电子传递速率(JF)、光合电子流的光化学传递速率(JC)、Rubisco羧化速率(VC)和TPU下降，并随生育时期推进其下降趋势更为明显，但高氮叶片的上述参数无显著变化；小麦叶片JC/JF、VC/JC和V0 /VC随氮素水平和大气CO2浓度的变化无显著变化，表明施氮能提高光合机构对光合能量的传递速率，但对光合能量的分配方向无明显影响。施氮提高小麦叶片氮素和叶绿素含量，并且使高大气CO2浓度下光合氮素利用效率(NUE)明显增加。大气CO2浓度升高后，施氮增强光合机构的光合能量运转速率，同化力提高，无明显的光合作用适应现象；由于氮素水平与大气CO2浓度对小麦叶片的光合能量利用存在明显的交互作用，而且高大气CO2浓度下施氮使得小麦叶片NUE增加、正常大气CO2浓度下降低，证明高大气CO2浓度下施氮对光合作用具有直接的影响。

关键词: 高大气CO2浓度, 氮素, 光合气体变换, 光能传递分配, 小麦

Abstract: Nitrogen application rate is a critical factor led to photosynthesis acclimation of C3 plant under elevated atmospheric CO2 concentration. However, current knowledge is inadequate for the responses of photosynthetic electron transport and energy distribution of photosynthesis acclimation to nitrogen application rate in C3 plant, and the influence of photosynthesis function on photosynthetic electron transport and energy distribution. Using Top Open Chambers, the elevated concentration of atmospheric CO2 was simulated. Wheat (Triticum aestivum L.) was grown under two nitrogen application rates and two atmospheric CO2 concentrations. The photosynthetic gas exchange parameters, chlorophyll fluorescence parameters, and chlorophyll content of wheat leaves, were measured at jointing and heading stages to study the influence of nitrogen application on photosynthetic function of wheat leaves exposed to elevated atmospheric CO2 concentration. The results showed that the photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr) decreased in low-N wheat leaves, and the photosynthetic acclimation appeared. However, in high-N wheat leaves the photosynthetic acclimation did not appear, Pn, Gs, and Tr decreased significantly whereas the leaf WUE increased significantly under elevated atmospheric CO2 concentration. The photochemical rate, photosynthetic electron rate of PSII (JF), electronic transport rate of photochemistry (JC), Rubisco carboxylase rate (VC), and triose phosphate utilization (TPU) declined significantly in low-N wheat leaves; and were not changed in high-N wheat leaves compared to those under ambient atmospheric CO2 concentration. The JC/JF, VC/JC, and V0/VC had no significant changes between treatments with different nitrogen application rate and atmospheric CO2 concentrations. This indicated that nitrogen application may increase the photosynthetic energy use, but have no significant influence on photosynthetic energy distribution. The leaf nitrogen and chlorophyll contents increased when nitrogen was applicated under both treatments of CO2 concentrations, especially, the photosynthetic nitrogen use efficiency (NUE) in high-N wheat leaves increased under elevated atmospheric CO2 concentration. It suggested that the photosynthetic energy transport rate and assimilatory ability increased by nitrogen application under elevated atmospheric CO2 concentration. Thus, the photosynthesis acclimation did not appear in high-N wheat leaves. Because there was a significant interaction between nitrogen application rate and atmospheric CO2 concentration on photosynthetic energy use in wheat leaves, and the photosynthetic NUE in high-N wheat leaves increased under elevated atmospheric CO2 concentration but decreased under normal ambient atmospheric CO2 concentration, it concluded that the nitrogen application affects photosynthesis directly in wheat leaves under elevated atmospheric CO2 concentration.

Key words: Atmospheric CO2 enrichment, Nitrogen, Photosynthetic gas exchange, Photosynthetic energy transport and districution, Wheat

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