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作物学报 ›› 2010, Vol. 36 ›› Issue (09): 1506-1511.doi: 10.3724/SP.J.1006.2010.01506

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

一个水稻长护颖突变体的遗传分析和基因定位

陈代波,占小登**,吴超,沈希宏,吴伟明,高志强,程式华*,曹立勇*   

  1. 中国水稻研究所 / 国家水稻改良中心 / 国家水稻生物学重点实验室，浙江杭州 310006
  • 收稿日期:2010-03-08 修回日期:2010-04-23 出版日期:2010-09-12 网络出版日期:2010-06-11
  • 通讯作者: 曹立勇, E-mail: caolycgf@mail.hz.zj.cn; 程式华, E-mail: shcheng@mail.hz.zj.cn
  • 基金资助:
    本研究由国家高技术研究发展计划(863计划)项目(2006AA10Z1E8)，农业部引进国际先进农业科学技术计划(948计划)项目(2006-G51)，国家自然科学基金项目(30623006)，转基因生物新品种培育重大专项(2008ZX08001-002)资助。

Genetic Analysis and Gene Mapping of a Long Empty Glumes Mutant in Rice (Oryza sativa L.)

CHEN Dai-Bo,ZHAN Xiao-Deng**,WU Chao,SHEN Xi-Hong,WU Wei-Ming,GAO Zhi-Qiang,CHENG Shi-Hua*,CAO Li-Yong*   

  1. China National Rice Research Institute / National Center for Rice Improvement / State Key Laboratory of Rice Biology, Hangzhou 310006, China
  • Received:2010-03-08 Revised:2010-04-23 Published:2010-09-12 Published online:2010-06-11
  • Contact: CAO Li-Yong,E-mail:caolycgf@mail.hz.zj.cn;CHENG Shi-Hua,E-mail:shcheng@mail.hz.zj.cn

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2095

被引次数

11

摘要： 花器官发育异常突变体是研究植物花发育分子机理的重要材料。本研究在特种栽培稻品种“鸭血糯”中发现一个长护颖自然突变体，命名为Osleg (Oryza sativa long empty glumes)。组织细胞学分析表明，该突变体护颖的远轴表皮细胞凸凹不平，毛状体较多，许多瘤状体轴向平行排列，与外稃表皮细胞结构相似。遗传分析结果表明，该突变性状受一对隐性基因控制。将Osleg纯合体与籼稻品种9311杂交构建F2定位群体，利用已公布的水稻SSR标记和自行设计的STS标记对突变位点进行基因定位，最终将OsLEG定位在水稻7号染色体短臂上的LC15和LC25标记之间，物理距离约207 kb，为进一步克隆OsLEG基因和研究禾本科植物花器官的分子调控机理提供了重要科学依据。

关键词: 水稻（Oryza sativa L.）, 花器官, 长护颖突变体, 基因定位

Abstract: Flowerdevelopment plays an important role in the life cycle of plant. However, the molecular mechanism of floral organ development has not been clear in rice (Oryza sativa L.). The mutants with abnormal flower organ were important materials for researching the flower development. In this study, a spontaneous mutant named Osleg, with two long empty glumes, was discovered from special rice cultivar Buxuenuo. Using scanning electron micrograph, we observed that the abaxial surface of the mutant Oslegrich in trichomes was rough and some tubercles were arranged in parallel, which has the similar structure to the epidermic cells of the lemma. The results suggested that the mutant traits could be evolved from the two lemmas during the evolution of Oryza. To mapping the gene OsLEG, we used mutant Osleg and 9311 as parents to structure F2 population. On the basis of the field observation, the ratio of normal empty glumes to long empty glumes was 4302:1485, fitting the 3:1 ratio [χ2(0.246)< χ20.05(3.84)]. Genetic analysis showed that the mutant character was controlled by a single recessive gene. Ultimately using the published SSR markers and designed some STS makers, the OsLEG was mapped to a 207-kb region between the STS markers LC15 and LC25 on the short arm of chromosome 7 across two BAC clones. With in this region, there were forty-six predicted genes in the rice database [TIGR release 5(RAP2 build 4.0)]. None of them was related directly to glumes in rice. But, one encoded F-box protein had connection with floral development and could be related to the OsLEG to a certain extent. Those studies will pave a way for the OsLEG cloning and study on the molecular genetic mechanisms of the floral organ in monocot.

Key words: Rice(Qryza sativa L.), Floral organ, Long empty glumes mutant, Gene location

[1] Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development
[J].Nature.1991, 353:31-37  
[2] Colombo L, Franken J, Koetje E, van Went J, Dons H J M, Angenent G C, van Tunen A J. The petunia MADS box gene FBP11 determines ovule identity
[J].Plant Cell.1995, 7:1859-1868  
[3] Angenet G C, Franken J, Busscher M, van Dijken A, van Went L, Dons H J M, van Tunen J. A novel class of MADS box genes is involved in ovule development in petunia
[J].Plant Cell.1995, 7:1569-1582  
[4] Pelaz S, Ditta G S, Baumann E, Wisman E, Yanofsky M F. B and C floral organ identity functions require SEPALLATA MADS- box genes
[J].Nature.2000, 405:200-203  
[5] Theissen G, Becker A, Di Rosa A, Kanno A, Kim J T, Munster T, Winter K U, Saedler H. A short history of MADS-box genes in plants
[J].Plant Mol Biol.2000, 42:115-149  
[6] Lohmann J U, Weigel D. Building beauty: the genetic control of floral patterning
[J].Dev Cell.2002, 2:135-142  
[7] Lim J, Moon Y H, Ann G, Jang S K. Two rice MADS domain proteins interact with OsMADS1
[J].Plant Mol Biol.2000, 44:513-527   
[8] Kyozuka J, Kobayashi T, Morita M, Shimamoto K. Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes. Plant Cell Physiol, 2000, 41: 710-718  
[9] Jia H, Chen R, Cong B, Cao K, Sun C, Luo D. Characterization and transcriptional profiles of two rice MADS-box genes
[J].Plant Sci.2000, 155:115-122  
[10] Moon Y H, Jung J Y, Kang H G, An G. Identification of a rice APETALA3 homologue by yeast two-hybrid screening
[J].Plant Mol Biol.1999, 40:167-177  
[11] Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y. SUPERWOMAN 1 and DROOPING LEAF genes control floral organ identity in rice
[J].Development.2003, 130:705-718  
[12] Chung Y Y, Kim S R, Kang H R. Characterization of two rice MADS-box genes homologous to GLOBOSA
[J].Plant Sci.1995, 109:45-56  
[13] Yamaguchi T, Nagasawa N, Kawasaki S, Matsuoka M, Nagato Y, Hirano H K. The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa
[J].Plant Cell.2004, 16:500-509  
[14] Kang H G, Jeon J S, Lee S, An G. Identification of class B and class C floral organ identity genes from rice plants
[J].Plant Mol Biol.1998, 38:1021-1029  
[15] Yamaguchi T, Lee D Y, Miyao A, Hirochika H, An G, Hirano H Y. Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa
[J].Plant Cell.2006, 18:15-28 
[16] Lopez-Dee Z P, Wittich P, Enrico Pe M, Rigola D, Buono I D, Gorla M S, Kater M M, Colombo L. OSMADS13, a novel rice MADS-box gene expressed during ovule development
[J].Dev Genet.1999, 25:237-244   
[17] Jeon J S, Jang S, Lee S, Nam J, Kim C, Lee S H, Chung Y Y, Kim S R, Lee Y H, Cho Y G, An G. Leafy hull sterile1 is a homeotic mutation in a rice MADS box gene affecting rice flower development
[J].Plant Cell.2000, 12:871-884  
[18] Prasad K, Parameswaran S, Vijayraghavan U. OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs
[J].Plant J.2005, 43:915-928  
[19] Lu Y-J(卢扬江), Zheng K-L(郑康乐). A simple method for isolation of rice DNA. Chin J Rice Sci (中国水稻科学), 1992, 6(1): 47-48 (in Chinese)  
[20] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregation analysis: a rapid method to detect markers in specific genomic regions by using segregating populations
[J].Proc Natl Acad Sci USA.1991, 88:9828-9832  
[21] Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newberg L. Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations
[J].Genomics.1987, 1:174-181 
[22] Bommert P, Satoh-Nagasawa N, Jackson D, Hirano H Y. Gene- tics and evolution of inflorescence and flower development in grasses
[J].Plant Cell Physiol.2005, 46:69-78  
[23] Zanis M J. Grass spikelet genetics and duplicate gene comparisons
[J].Int J Plant Sci.2007, 168:93-110  
[24] Ambrose B A, Lerner D R, Ciceri P, Padilla C M, Yanofsky M F, Schmidt R J. Molecular and genetic analyses of the silky1 gene reveal conservation in floral organ specification between eudicots and monocots
[J].Mol Cell.2000, 5:569-579  
[25] Whipple C J, Ciceri P, Padilla C M, Ambrose B A, Bandong S L, Schmidt R J. Conservation of B-class floral homeotic gene function between maize and Arabidopsis
[J].Development.2004, 131:6083-6091   
[26] Thompson B E, Hake S. Translational biology: from Arabidopsis flowers to grass Inflorescence architecture
[J].Plant Physiol.2009, 149:38-45  
[27] Sajo M G, Longhi-Wagner H M, Rudall P J. Reproductive morphology of the early-divergent grass Streptochaeta and its bearing on the homologies of the grass spikelet
[J].Plant Syst Evol.2008, 275:245-255  
[28] Preston J C, Christensen A, Malcomber S T, Kellogg E A. MADS-box gene expression and implications for developmental origins of the grass spikelet
[J].Am J Bot.2009, 96:1419-1429  
[29] Wang H J, Huang X Q, Roder M S, Borner A. Genetic mapping of loci determining long glumes in the genus Triticum
[J].Euphytica.2002, 123:287-293  
[30] Watanabe N, Imamura I. Genetic control of long glume phenotype in tetraploid wheat derived from Triticum petropavlovskyi Udacz
[J].et Migusch. Euphytica.2002, 128:211-217  
[31] Watanabe N. Genetic control of the long glume phenotype in tetraploid wheat by homoeologous chromosomes
[J].Euphytica.1999, 106:39-43 
[32] Arber A. The Gramineae: a study of cereal, bamboo, and grasses
[J].Nature.1935, 136:317-319  
[33] Callis J, Vierstra R D. Protein degradation in signaling
[J].Curr Opin Plant Biol.2000, 3:381-386
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