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作物学报 ›› 2010, Vol. 36 ›› Issue (12): 2191-2195.doi: 10.3724/SP.J.1006.2010.02191

• 研究简报 • 上一篇    

利用SSR分子标记分析茶树地方品种的遗传多样性

王丽鸳1,姜燕华1,段云裳2,成浩1,*,周健1,曾建明1,韦康1   

  1. 1中国农业科学院茶叶研究所 / 国家茶树改良中心，浙江杭州 310008；2南京农业大学，江苏南京 210095
  • 收稿日期:2010-05-18 修回日期:2010-08-02 出版日期:2010-12-12 网络出版日期:2010-10-09
  • 通讯作者: 成浩, E-mail: chenghao@mail.tricaas.com
  • 基金资助:

    本研究由现代农业产业技术体系建设专项(nycytx-23)资金资助。

Genetic Diversity of Tea Landraces Using SSR Markers

WANG Li-Yuan1,JIANG Yan-Hua1,DUAN Yun-Shang2,CHENG Hao1,*,ZHOU Jian1,ZENG Jian-Ming1,WEI Kang1   

  1. 1 Tea Research Institute, Chinese Academy of Agricultural Sciences / National Center for Tea Improvement, Hangzhou 310008, China; 2 Nanjing Agricultural University, Nanjing 210095, China
  • Received:2010-05-18 Revised:2010-08-02 Published:2010-12-12 Published online:2010-10-09
  • Contact: CHENG Hao,E-mail:chenghao@mail.tricaas.com

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2433

被引次数

28

摘要： 正确评价茶树地方品种的遗传多样性是有效保护和利用茶树地方品种的前提条件。本研究从西湖龙井群体种中选取91个单株，用SSR分子标记分析其遗传多样性。采用计算机模拟方法，探讨了抽样群体样本量、SSR引物等位基因数影响茶树地方品种的主要遗传多样性参数值的变化规律。结果表明，样本量对茶树地方品种的遗传多样性参数值有不同程度的影响，当样本量达到15个单株时，各遗传参数值趋于稳定；SSR引物等位基因数对茶树地方品种各遗传多样性参数值的影响很大，而且达到总体遗传多样性90%所需的样本量也很不一样。当SSR引物等位基因数为5时，24个茶树单株才能达到茶树地方品种总体90%以上的遗传变异。本研究为茶树地方品种遗传多样性的评价和采用合理的保护策略提供了科学依据。

关键词: SSR标记, 茶树, 遗传多样性, 取样策略

Abstract: Appropriate assessment of genetic diversity in tea landraces is the prerequisite of conservation and utilization of these valuable genetic resources. Ninety-one individual tea plants, from Longjing tea landrace population, were used in this study. Computer simulation was utilized to study the changes of genetic diversity parameters influenced by the number of samples and alleles per SSR locus. The results showed that the sampling number differentially affected the genetic diversity parameters. Only when the sample number was higher than 15, the parameters tended to be stable. There were also large effects of the number of alleles per SSR locus on the genetic diversity, which was closely associated with the sample number. It was found when the number of alleles per SSR locus was five, at least 24 individual tea plants were needed for reaching to 90% of the total genetic diversity of tea landraces. The results are useful for providing scientific basis for the assessment of genetic diversity and appropriate conservation strategies of tea landraces.

Key words: SSR, Tea landraces, Genetic diversity, Sampling strategy

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