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作物学报 ›› 2011, Vol. 37 ›› Issue (04): 603-611.doi: 10.3724/SP.J.1006.2011.00603

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆耐旱选择群体QTL定位

李灿东1,2,3,蒋洪蔚1,刘春燕1,郭泰2,王志新2,吴秀红2,郑伟2,邱鹏程3,张闻博3,宋英博2,栾奕娜5,陈庆山3,*,胡国华1,4,*   

  1. 1黑龙江省农垦科研育种中心，黑龙江哈尔滨 150090；2黑龙江省农科院佳木斯分院，黑龙江佳木斯 154007；3东北农业大学农学院，黑龙江哈尔滨 150030；4国家大豆工程技术研究中心，黑龙江哈尔滨 150050；5黑龙江农业职业技术学院，黑龙江佳木斯 154007
  • 收稿日期:2010-09-20 修回日期:2011-01-06 出版日期:2011-04-12 网络出版日期:2011-02-24
  • 通讯作者: 陈庆山, E-mail: qshchen@126.com, 0451-55191945; 胡国华, E-mail: Hugh757@vip.163.com, 0451-55199475
  • 基金资助:

    本研究由转基因专项大豆导入系构建及有利隐蔽基因挖掘(2009ZX08009-013B)，国家公益性行业(农业)科研专项(200903003)和引进国际先进农业科学技术计划(948计划)项目(2006G01)资助。

QTL Identification of Drought Tolerance to Soybean in Selection Population

LI Can-Dong1,2,3,JIANG Hong-Wei1,LIU Chun-Yan1,GUO Tai2,WANG Zhi-Xin2,WU Xiu-Hong2,ZHENG Wei2,QIU Peng-Cheng3,ZHANG Wen-Bo3,SONG Ying-Bo2,LUAN Yi-Na5,CHEN Qing-Shan3,*,HU Guo-Hua1,4,*   

  1. 1 Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090, China; 2 Jiamusi branch institute, Heilongjiang Academy of Agricultural Sciences, Jiamusi 154007, China; 3 College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 4The National Research Center of Soybean Engineering and Technology, Harbin 150050, China; 5 Heilongjiang Agricultural Vocational and Technical College, Jiamusi 154007, China
  • Received:2010-09-20 Revised:2011-01-06 Published:2011-04-12 Published online:2011-02-24
  • Contact: 陈庆山, E-mail: qshchen@126.com, 0451-55191945; 胡国华, E-mail: Hugh757@vip.163.com, 0451-55199475

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被引次数

10

摘要： 以红丰11为轮回亲本、Clark为供体亲本构建回交群体进行耐旱性鉴定，对获得选择群体进行全基因组SSR标记扫描，计算供体基因型导入频率，利用卡方测验检测偏分离SSR位点，并结合GGT软件对各连锁群分析, 对5个耐旱相关性状进行QTL定位。以卡方测验检测到23个SSR偏分离位点(超导入)，分布于10条连锁群。方差分析表明，8个叶片持水能力QTL分布于A1、B1、C2、E、L和N连锁群；9个根长QTL分布于C2、F、G和I连锁群；11个根干重QTL分布于A2、B1、B2、E、F、K、L、M和O连锁群；12个产量QTL分布于B1、D1a、E、F、G、I、L、M和O连锁群；7个生物量QTL分布于E、F、G、K、L和N连锁群。在E连锁群的Sat_136位点，对于叶片持水能力、根干重、产量和生物量具有一致性；在F连锁群的GMRUBP位点，对于根干重和生物量具有一致性，Satt586位点，对于根长、根干重和产量具有一致性；在K连锁群的Satt167位点，对于根干重和生物量具有一致性，SOYPRP1位点，对于根长和生物量具有一致性；在L连锁群的Satt398位点，对于根长和产量具有一致性，Satt694位点对于叶片持水能力和生物量具有一致性；在M连锁群的GMSL514位点，对于根干重和产量具有一致性；以上位点均与卡方测验检测到的“超导入”位点具有一致性。经过供体等位基因卡方测验和耐旱QTL定位，共检测到33个QTL，其中有17个同时被检测到。这些位点可能是控制大豆耐旱性的重要位点。

关键词: 大豆, 耐旱性, QTL分析

Abstract: A primary backcross introgression of soybean population was constructed by using Hongfeng 11 as recurrent parent and Clark as donor parent. After screening under drought stress, the genotypes of selective population were obtained with the whole genome SSR markers, and the frequency of donor genes segments were analyzed. QTLs of five drought-tolerance traits were mapped by Chi-testcombined GGT linkage group analysis. In total, 23 SSR excessive introgression loci on 10 chromosomes were detected with χ2test. The QTL identification was conducted by one-way ANOVA (for single marker analysis, P<0.01). Eight QTLs of RWC (relative water content) were located on A1, B1, C2, E, L, and N linkage groups, nine QTLs of RRL (relative root length) on C2, F, G, and I linkage groups, 11 QTLs of RRW (relative root dry weight) on A2, B1, B2, E, F, K, L, M, and O linkage groups, 12 QTLs of RGY (relative grain yield) on B1, D1a, E, F, G, I, L, M, and O linkage groups and seven QTLs of RMB (relative microbial biomass) on E, F, G, K, L, and N linkage groups. The QTL at Sat_136 on E linkage group was identical for RWC, RRW, RGY, and RMB, and QTL at GMRUBP on F linkage group for RRW and RMB, QTL at Satt586 on F linkage group for RRL, RRW, and RGY, QTL at Satt167 on K linkage group for RRW and RMB, QTL at SOYPRP1 on K linkage group for RRL and RMB, QTL at Satt398 on L linkage group for RRL and RGY, QTL at Satt694 on L linkage group for RWC and RMB, QTL at GMSL514 on L linkage group for RRW and RGY. All above QTLs were coincident with those detected by excessive introgression of χ2 test. Thirty-three QTLs were mapped by χ2 test or one-way ANOVA, and among them 17 QTLs were detected by both methods. So these QTLs should be essential for drought tolerance. The results provide a foundation for fine mapping, cloning and molecular breeding of favorable genes related with drought tolerance.

Key words: Soybean, Drought tolerance, QTL identification

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