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作物学报 ›› 2011, Vol. 37 ›› Issue (05): 842-854.doi: 10.3724/SP.J.1006.2011.00842

• 耕作栽培·生理生化 • 上一篇    下一篇

氮肥运筹对大穗型水稻品种金恢809灌浆期叶片蛋白质表达的影响

张志兴1,2,李忠1,2,陈军1,2,李奇松1,2,陈龙怀1,2,陈鸿飞1,2,黄锦文1,林文雄1,2,*   

  1. 1 福建农林大学农业生态研究所, 福建福州350002; 2 福建农林大学生命科学学院, 福建福州350002
  • 收稿日期:2010-10-28 修回日期:2011-03-08 出版日期:2011-05-12 网络出版日期:2011-03-24
  • 通讯作者: 林文雄, E-mail: wenxiong181@163.com, Tel: 0591-83769440
  • 基金资助:

    本研究由国家自然科学基金项目(30871494)，教育部高等学校博士学科点专项科研基金项目(200803890006)和福建省自然科学基金项目(2007J0304, 2008J0042)资助。

Effects of Nitrogen Management on Protein Expression of Flag Leaves during Grain-Filling Period in Large Panicle Rice (Oryza sativa L.)

ZHANG Zhi-Xing1,2,LI Zhong1,2,CHEN Jun1,2,LI Qi-Song1,2,CHEN Long-Huai1,2,CHEN Hong-Fei1,2,HUANG Jin-Wen1,LIN Wen-Xiong1,2,*   

  1. 1 Institute of Agricultural Ecology, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 2 School of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China
  • Received:2010-10-28 Revised:2011-03-08 Published:2011-05-12 Published online:2011-03-24
  • Contact: 林文雄, E-mail: wenxiong181@163.com, Tel: 0591-83769440

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被引次数

30

摘要： 籽粒灌浆期叶片的代谢情况对水稻最终产量的形成具有重要的意义。本文运用双向电泳技术探讨了两种不同氮肥施用比例下，籽粒灌浆不同时期叶片蛋白的差异表达情况。共检测到32个出现差异表达的蛋白，其中27个在后期适当增加氮肥施用比例的处理下上调表达，5个下调表达。依据蛋白功能可以将鉴定到的蛋白分为5大类即光合代谢(12个)、抗逆反应(5个)、激素合成及信号转导(5个)、细胞生长和分化(5个)、假想蛋白(5个)。为了进一步明确氮肥调控对灌浆期叶片的影响，本文考察了叶片光合以及保护酶相关指标。结果显示，增加水稻生育后期的氮肥施用比例，延缓了叶片中叶绿素以及可溶性蛋白在籽粒灌浆期的降解，延长了叶片光合作用时间，提高了SOD、POD和CAT在灌浆后期的活性，降低了膜脂过氧化程度。生理指标结果进一步证明了差异蛋白组学结果的可靠性，说明后期增加氮素的供应确能有效地促进叶片灌浆期正常的生理代谢。本研究在蛋白水平和生理指标水平，为进一步揭示氮肥调控措施对水稻灌浆代谢机理的影响提供了理论依据。

关键词: 水稻, 籽粒灌浆, 叶片, 蛋白, 氮肥

Abstract: In rice (Oryza sativa), approximately 60%–100% of the carbon in mature grains originates from CO2 assimilation during the grain-filling period, with the flag leaf as the most important contributor to the yield. It is therefore important to understand molecular mechanisms of flag leaf during the grain-filing period. Two-dimensional electrophoresis (2-DE) technology was applied to investigate the differential expression patterns of leaf proteins from different periods during grain-filling process in rice cultivation Jinhui 809 treated by two different nitrogen application ratios. The results showed 32 differentially expressed proteins with 27 up-regulated and five down-regulated, in response to increased nitrogen application at grain-filling stage. According to their relative functions the identified proteins were classified into five main categories: photosynthesis (12); adversity-resistance response (5); hormone synthesis and signal transduction (5); cell growth and differentiation (5); unknown functions (5). Then photosynthesis-related features together with adversity-defense related indicators (SOD, POD, CAT, MDA, and soluble protein) were determined. The appropriate increase in nitrogen application at late growth stage on one hand delayed the degradation of chlorophyll and soluble protein in leaf during grain filling period, in turn elongated the leaf photosynthesis process; on the other hand promoted the activities of SOD, POD, CAT, while decreased the lipid peroxidation degree, which together indicated the strengthen of leaf adversity-defense ability. The results not only verify the high efficient effect of increased nitrogen application on leaf metabolisms during grain-filling process, but also provide the theoretic basis for studies on rational management of nitrogen fertilizer and its relevant molecular regulation in the future.

Key words: Rice, Grain-filling, Flag leaf, Protein, Nitrogen fertilizer

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