作物学报 ›› 2011, Vol. 37 ›› Issue (05): 842-854.doi: 10.3724/SP.J.1006.2011.00842
张志兴1,2,李忠1,2,陈军1,2,李奇松1,2,陈龙怀1,2,陈鸿飞1,2,黄锦文1,林文雄1,2,*
ZHANG Zhi-Xing1,2,LI Zhong1,2,CHEN Jun1,2,LI Qi-Song1,2,CHEN Long-Huai1,2,CHEN Hong-Fei1,2,HUANG Jin-Wen1,LIN Wen-Xiong1,2,*
摘要: 籽粒灌浆期叶片的代谢情况对水稻最终产量的形成具有重要的意义。本文运用双向电泳技术探讨了两种不同氮肥施用比例下,籽粒灌浆不同时期叶片蛋白的差异表达情况。共检测到32个出现差异表达的蛋白,其中27个在后期适当增加氮肥施用比例的处理下上调表达,5个下调表达。依据蛋白功能可以将鉴定到的蛋白分为5大类即光合代谢(12个)、抗逆反应(5个)、激素合成及信号转导(5个)、细胞生长和分化(5个)、假想蛋白(5个)。为了进一步明确氮肥调控对灌浆期叶片的影响,本文考察了叶片光合以及保护酶相关指标。结果显示,增加水稻生育后期的氮肥施用比例,延缓了叶片中叶绿素以及可溶性蛋白在籽粒灌浆期的降解,延长了叶片光合作用时间,提高了SOD、POD和CAT在灌浆后期的活性,降低了膜脂过氧化程度。生理指标结果进一步证明了差异蛋白组学结果的可靠性,说明后期增加氮素的供应确能有效地促进叶片灌浆期正常的生理代谢。本研究在蛋白水平和生理指标水平,为进一步揭示氮肥调控措施对水稻灌浆代谢机理的影响提供了理论依据。
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