水稻细卷叶突变体nrl2(t)的遗传分析和基因定位

doi:10.3724/SP.J.1006.2011.01159

摘要/Abstract

摘要： 研究调控水稻叶片发育基因对水稻功能基因组学和株型改良有着重要的意义。本研究从籼稻恢复系缙恢10号的EMS突变体库中发现一个水稻新型突变体，命名为nrl2_(t)。该突变体叶片卷曲、变细、伸长，茎秆变细，抽穗期提前，叶绿素含量增高，孕穗期剑叶生长素含量降低，而幼穗中生长素含量有所提高。遗传分析表明该性状受一对隐性基因控制。利用SSR标记将该基因定位于第3染色体SSR标记s3RM1和s3RM3之间，物理距离约为114 kb。研究结果为该基因的克隆及进一步揭示细叶卷曲形成的分子机理奠定了基础。

关键词: 水稻(Oryza sativa L.), 细卷叶, 遗传分析, 基因定位, 生长素

Abstract: Study of the genes which regulate leaf development is significant in rice functional genomics research and molecular breeding. In the present study, a novel rolled and narrow leaf mutant was obtained from the rice (Oryza sativa L.) restorer line Jinhui 10 treated by ethyl methyl sulphonate (EMS), named nrl2_(t). The mutant exhibited narrow/semi-rolled and elongate leaves, earlier flowering, thinner stalk, higher pigment content and higher plant height compared with the control. The indole-3-acetic acid (IAA) content relatively reduced in the flag leaf compared to that of wild type, but increased in the young panicle at the heading stage. Genetic analysis indicated that the mutant was controlled by a single recessive gene, located on the chromosome 3 between SSR markers s3RM1 and s3RM3, delimiting to a 114 kb region. These results will lay a good foundation for molecular cloning and functional analysis of the NRL2_(t).

Key words: Rice (Oryza sativa L.), Narrow and rolled leaf, Genetic analysis, Gene mapping, IAA

王德仲, 桑贤春, 游小庆, 王增, 王秋实, 赵芳明, 凌英华, 李云峰, 何光华. 水稻细卷叶突变体nrl2_(t)的遗传分析和基因定位[J]. 作物学报, 2011, 37(07): 1159-1166.

WANG De-Zhong, SANG Xian-Chun, LIU Xiao-Qiang, WANG Ceng, WANG Qiu-Shi, DIAO Fang-Meng, LING Yang-Hua, LI Yun-Feng, HE Guang-Hua. Genetic Analysis and Gene Mapping of a Novel Narrow and Rolled Leaf Mutant nrl2(t) in Rice (Oryza sativa L.)[J]. Acta Agron Sin, 2011, 37(07): 1159-1166.

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