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作物学报 ›› 2011, Vol. 37 ›› Issue (07): 1167-1174.doi: 10.3724/SP.J.1006.2011.01167

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

引自美国的大豆资源抗疫霉根腐病基因分析

夏长剑,张吉清,王晓鸣,刘章雄,朱振东*   

  1. 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程，北京100081
  • 收稿日期:2011-01-30 修回日期:2011-04-12 出版日期:2011-07-12 网络出版日期:2011-05-11
  • 通讯作者: 朱振东, E-mail: zhuzd115@caas.net.cn, Tel: 010-82109609
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(3-20)，农业部作物种质资源保护子项目(NB09-2130135-1-15)和中央级公益性科研院所基本科研业务费专项(082060302-06)资助。

Analysis of Genes Resistance to Phytophthora Root Rot in Soybean Germplasm Imported from America

XIA Chang-Jian,ZHANG Ji-Qing,WANG Xiao-Ming,LIU Zhang-Xiong,ZHU Zhen-Dong*   

  1. Institute of Crop Sciences / National Key Facility for Crop Genetic Resources and Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-01-30 Revised:2011-04-12 Published:2011-07-12 Published online:2011-05-11
  • Contact: 朱振东, E-mail: zhuzd115@caas.net.cn, Tel: 010-82109609

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被引次数

6

摘要： 大豆疫霉根腐病是影响中国大豆生产的主要病害之一, 利用抗病品种是防治该病最经济有效的方法。本研究通过下胚轴创伤接种鉴定了13个大豆疫霉菌株在从美国引进的85个大豆品种(系)上的反应，结果表明, 72个品种(系)抗1个到12个大豆疫霉菌株。通过与14个含有单个已知抗疫霉根腐病基因的大豆品种(系)的反应型比较并结合系谱分析，明确35个品种(系)分别含有Rps1a、Rps1c、Rps1k、Rps2、Rps3c、Rps4、Rps5、Rps6和Rps7抗病基因或基因组合，其中有14个品种(系)含有Rps1a，1个含有Rps1c和2个含有Rps1k，这3个基因能够有效抵御我国大豆疫霉种群，可以直接用于抗病育种。

关键词: 大豆资源, 大豆疫霉, 抗性基因, 基因推导

Abstract: Phytophthora root rot caused by Phytophthora sojae is a major constraint in soybean production in China. Breeding resistant cultivars is a most effective and economical method for the disease control. In this study, 85 soybean cultivars (lines) from America were inoculated with 13 strains of P. sojae using the hypocotyl inoculation technique and identified the reactions. The results showed that 72 cultivars (lines) were resistant to one or more P. sojae strains. Comparisons of reaction types of differential soybean cultivars (lines) and pedigree analyses allowed postulation of genes or combinations of genes for Rps1a, Rps1c, Rps1k, Rps2, Rps3c, Rps4, Rps5, Rps6,and Rps7 in 35 cultivars or lines. Of the 35 cultivars or lines, 14 carrying Rps1a, one carrying Rps1c and two carrying Rps1k could be used as resistant parents in soybean breeding programs.

Key words: Soybean germplasm, Phytophthora sojae, Resistance gene, Gene postulation

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