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作物学报 ›› 2012, Vol. 38 ›› Issue (03): 408-415.doi: 10.3724/SP.J.1006.2012.00408

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻两用核不育系龙S抗稻瘟病主效基因的定位

王建龙1,2，吴立群2，刘建丰1， 戴良英1，刘雄伦1，肖应辉1，谢红军3，刘群恩1，李婷1，贾先勇2，王国梁1,4,*，袁隆平5,*   

  1. 1湖南农业大学，湖南长沙 410128；2湖南金健种业有限责任公司，湖南常德 415000；3湖南省水稻研究所，湖南长沙 410125；4美国俄亥俄州州立大学，俄亥俄州哥伦布 43210；5国家杂交水稻工程技术研究中心，湖南长沙 410125
  • 收稿日期:2011-06-21 修回日期:2011-12-15 出版日期:2012-03-12 网络出版日期:2012-01-04
  • 通讯作者: 袁隆平, E-mail: lpyuan@hhrrc.ac.cn, Tel: 0731-82872998; 王国梁, E-mail: wang.620@osu.edu, Tel: 0731-84638423
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2010AA101306)和湖南农业大学引进人才基金项目(11YJ14)资助?

Mapping of the Resistant Gene to Rice Blast in the Dual Purpose Genic Male Sterile Rice, LongS

WANG Jian-Long1,2,WU Li-Qun2,LIU Jian-Feng1,DAI Liang-Ying1,LIU Xiong-Lun1,XIAO Ying-Hui1,XIE Hong-Jun3,LIU Qun-En1,LI Ting1,JIA Xian-Yong2,WANG Guo-Liang1,4,*,YUAN Long-Ping5,*   

  1. 1Hunan Agricultural University, Changsha 410128, China; 2Hunan Jinjian Seed Industry Co., Ltd, Changde 415000, China; 3Hunan Rice Research Institute, Changsha 410125, China; 4Ohio State University, Columbus 43210, USA; 5China National Hybrid Rice Research and Development Center, Changsha 410125, China
  • Received:2011-06-21 Revised:2011-12-15 Published:2012-03-12 Published online:2012-01-04
  • Contact: 袁隆平, E-mail: lpyuan@hhrrc.ac.cn, Tel: 0731-82872998; 王国梁, E-mail: wang.620@osu.edu, Tel: 0731-84638423

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摘要： 龙S是一个广谱抗稻瘟病的水稻两用核不育系，利用分子标记技术精细定位其主效抗性基因，对于培育抗稻瘟病水稻新品种具有重要意义。采用来自国内外的41个稻瘟病菌系通过接种鉴定方式对龙S进行了稻瘟病抗谱分析，结果显示龙S的抗性频率为100%，对其中39个菌系表现高水平抗性，与Pi9的携带品种75-1-127抗性频率和抗病级别基本相当。群体遗传分析表明龙S的抗性基因表现为显性遗传方式，对于不同菌系龙S表现出不同的抗病遗传模式，其中龙S对稻瘟菌系318-2的抗性由单基因控制。通过抗病亲本龙S与感病亲本日本晴构建F2分离群体，采用BSA (bulk segregant analysis)及RCA (recessive class analysis)分析方法，将龙S的主效抗病基因精细定位于第9染色体上的SSR标记M1-M2所在的1.31 cM区间，与已克隆的广谱抗稻瘟病基因Pi5位于相邻的染色体区域。抗谱分析表明，龙S与Pi5、Pii单基因系的抗性频率差异明显，抗谱较后二者更广。龙S主效抗性基因的精细定位，为进一步揭示其与Pi5、Pii的等位关系以及通过分子标记辅助选择培育抗病水稻新品种奠定了基础。

关键词: 水稻, 龙S, 稻瘟病, 抗性基因, 基因定位

Abstract: LongS is a dual purpose genic male sterile rice with broad-spectrumresistance to rice blast. The objective of the present study was to identifythe resistance spectrumto rice blast, to analyze thegenetic behavior ofresistance gene, and tomap the major resistancegenes in LongS. LongS had a resistance frequency of 100% inoculatedwith 41strainsofMagnaporthe oryza. Population geneticanalysis showed that theresistance genes in LongSexhibited dominant inheritance, the genetic model of R gene varied depend on the strainsof Magnaporthe oryzae. The main-effect resistant gene to rice blast was fine mapped, by using the bulk segregant analysis (BSA) and recessive class analysis (RCA) methods, with the F2 population derived from the resistant parent of LongS and the susceptible parent of Nipponbare. Asingle resistant gene to the race of 318-2 locatedon the interval flanked by the SSRmarkersof M1 and M2with a genetic distance of 1.3 cM onchromosome 9 were adjacent to the broad-spectrum blast resistance gene, Pi5. Both of the resistance spectrum and resistant frequency of LongS, however, were significantly different to those of resistant gene of Pi5 and Pii. In conclusion, the major-effect resistant gene identified in this study may be a new broad-spectrum blast resistance gene. The DNA markers linked to the new R gene identified in this study should be useful for marker-aided breeding of blast-resistant rice cultivars.

Key words: Rice, LongS, Rice blast, Resistant gene, Gene mapping

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