欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2011, Vol. 37 ›› Issue (09): 1525-1532.doi: 10.3724/SP.J.1006.2011.01525

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

不同施氮水平下水稻株高与抽穗期的QTL比较分析

冯跃，翟荣荣**，曹立勇，林泽川，魏兴华，程式华*   

  1. 中国水稻研究所 / 国家水稻改良中心 / 国家水稻生物学重点实验室, 浙江杭州 310006
  • 收稿日期:2010-12-31 修回日期:2011-04-27 出版日期:2011-09-12 网络出版日期:2011-06-28
  • 通讯作者: 程式华, E-mail: shcheng@mail.hz.zj.cn, Tel: 0571-63370188
  • 基金资助:

    本研究由农业部作物种质资源保护项目(NB2011-2130135-27)，中央级公益性科研院所专项资金资助项目(2009RG001-3)和浙江省重点科技创新团队项目(2010R50024-16)资助。

QTL Analysis for Plant Height and Heading Date in Rice under Two Nitrogen Levels

FENG Yue,ZHAI Rong-Rong**,CAO Li-Yong,LIN Ze-Chuan,WEI Xing-Hua,CHENG Shi-Hua*   

  1. China National Rice Research Institute / National Center for Rice Improvement / State Key Laboratory of Rice Biology, Hangzhou 310006, China
  • Received:2010-12-31 Revised:2011-04-27 Published:2011-09-12 Published online:2011-06-28
  • Contact: 程式华, E-mail: shcheng@mail.hz.zj.cn, Tel: 0571-63370188

PDF

1238

被引次数

26

摘要： 利用超级杂交稻协优9308 (协青早B×中恢9308)衍生的重组自交系(recombinant inbred line, RIL)群体及其分子连锁图谱, 应用Windows QTL Cartographer 2.5对施氮和不施氮条件下水稻株高(PH)和抽穗期(HD)进行了QTL分析。在2种氮水平下检测到9个株高QTL和8个抽穗期QTL, 检测到4个影响2种环境下株高和抽穗期差值的QTL, 单个QTL可解释的表型变异介于5.68%~18.40%之间；在第7染色体上RM5436附近和第8染色上RM5556~RM310区间检测到同时控制2种氮水平下株高和抽穗期的QTL, 各位点的遗传效应贡献率较大, 增效等位基因均来源于R9308, 适用于分子标记辅助育种和聚合育种。在第2染色体上RM5916~RM166区间和第8染色体上RM2366~RM5767区间分别检测到1个影响2种氮水平下抽穗期差值和1个株高差值的QTL可能对水稻的氮素高效利用有直接贡献。

关键词: 水稻, 氮水平, 株高, 抽穗期, QTL分析

Abstract: A recombinant inbred line (RIL), derived from a super hybrid rice cross of XQZB/R9308 and its genetic linkage map were used to identify QTLs through Windows QTL Cartographer 2.5 for plant height (PH) and heading date (HD) under low and normal nitrogen conditions in rice. Nine QTLs for PH and eight QTLs for HD were detected under two nitrogen levels, four QTLs for trait differences of PH and HD were detected between the two environments, explaining 5.68–18.40% of phenotypic variation. The pleiotropic QTLs influencing PH and HD were detected near RM5436 on chromosome 7 and in the interval RM5556–RM310 on chromosome 8, which showed high contribution of genetic effects and the position alleles were all originated from R9308. These QTLs are favorable for marker-assisted selection. One QTL for HD difference and one QTL for PH difference were detected in the interval RM5916–RM166 on chromosome 2 and in the interval RM2366–RM5767 on chromosome 8, which were considered to directly contribute to nitrogen use efficiency in rice.

Key words: Rice, Nitrogen level, Plant height, Heading date, QTL analysis

[1]Cassman K G, Peng S B, Olk D C, Ladha J K, Reichardt, Dobermann A, Singh U. Opportunities for increased nitrogen use efficiency from improved management in irrigated rice systems. Field Crops Res, 1998, 56: 7-39
[2]Vlek P L, Byrnes B H. The efficacy and loss of fertilizer N in lowland rice. Fert Res, 1986, 9: 131-147
[3]Fang P, Yu X M, Zhu R Q, Wu P. QTLs for rice leaf chlorophyll content under low N stress. Pedosphere, 2004, 14: 145-150
[4]Fang P(方萍), Tao Q-N(陶勤南), Wu P(吴平). QTLs underlying rice root to uptake NH4-N and NO3-N and rice N use efficiency at seedling stage. Plant Nutr Fert Sci (植物营养与肥料学报), 2001, 7(2): 159-165 (in Chinese with English abstract)
[5]Fang P, Wu P. QTL × N-level interaction for plant height in rice (Oriza sativa L.). Plant Soil, 2001, 236: 237-242
[6]Xing Y-Z(邢永忠), Xu C-G(徐才国), Hua J-P(华金平), Tan Y-F(谈移芳), Sun X-L(孙新立). Mapping and isolation of quantitative trait loci controlling plant height and heading date in rice. Acta Bot Sin (植物学报), 2001, 43(7): 721-726 (in Chinese with English abstract)
[7]Li Z K, Pinson S R M, Stansel J W, Park W D. Identification of quantitative trait loci (QTLs) for heading date and plant height in cultivated rice (Oryza sativa L.). Theor Appl Genet, 1995, 91: 374-381
[8]Mei H W, Luo L J, Ying C S, Wang Y P, Yu X Q, Guo L B, Paterson A H, Li Z K. Gene actions of QTLs affecting several agronomic traits resolved in a recombinant inbred rice population and two testcross populations. Theor Appl Genet, 2003, 107: 89-101
[9]Li Z K, Yu S B, Lafitte H R, Huang N, Courtois B, Hittalmani S, Vijayakumar C H M, Liu G F, Wang G C, Shashidhar H E, Zhuang J Y, Zheng K L, Singh V P, Sidhu J S, Srivantaneeyakul S, Khush G S. QTL × environment interactions in rice. I. Heading date and plant height. Theor Appl Genet, 2003, 108: 141-153
[10]Ma L-Y(马良勇), Bao J-S(包劲松), Li X-M(李西明), Zhu X-D(朱旭东), Ji Z-J(季芝娟), Xia Y-W(夏英武), Yang C-D(杨长登). Progress on cloning and functional analysis of dwarfism related genes in rice. Chin J Rice Sci (中国水稻科学), 2009, 23(1): 1-11 (in Chinese with English abstract)
[11]Yang D-W(杨德卫), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Ling J(林静), Chen T(陈涛), Zhu W-Y(朱文银), Wang C-L(王才林). Mapping and genetic analysis of quantitative trait loci for heading date with chromosome segment substitution lines in Oryza sativa. Chin Bull Bot (植物学报), 2010, 45(2): 189-197 (in Chinese with English abstract)
[12]Feng Y, Cao L Y, Wu W M, Shen X H, Zhan X D, Zhai R R, Chen D B, Cheng S H. Mapping QTLs for nitrogen-deficiency tolerance at seedling stage in rice (Oryza sativa L.). Plant Breed, 2010, 129: 652-656
[13]Shen X-H(沈希宏), Chen S-G(陈深广), Cao L-Y(曹立勇), Zhan X-D(占小登), Chen D-B(陈代波), Wu W-M(吴伟明), Cheng S-H(程式华). Construction of genetic linkage map based on a RIL population derived from super hybrid rice. Mol Plant Breed (分子植物育种), 2008, 6(5): 861-866 (in Chinese with English abstract)
[14]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL Nomenclature. Rice Genet Newsl, 1997, 14: 11-13
[15]Senaratne R, Ratnasinghe D S. Nitrogen fixation and beneficial effects of some grain legurnes and green-manure crops on rice. Boil Fert Soils, 1995, 19: 49-54
[16]Cao G-L(曹桂兰), Zhang Y-Y(张媛媛), Piao Z-Z(朴钟泽), Han L-Z(韩龙植). Evaluation of tolerance to low N-fertilizered level for rice type. J Plant Genet Resour (植物遗传资源学报), 2006, 7(3): 316-320 (in Chinese with English abstract)
[17]Piao Z-Z(朴钟泽), Han L-Z(韩龙植), Gao X-Z(高熙宗). Variations of nitrogen use efficiency by rice genotype. Chin J Rice Sci (中国水稻科学), 2003, 17(3): 233-238 (in Chinese with English abstract)
[18]Jiang L-G(江立庚), Dai T-B(戴廷波), Wei S-Q(韦善清), Gan X-Q(甘秀芹), Xu J-Y(徐建云), Cao W-X(曹卫星). Genotypic differences and valuation in nitrogen uptake and utilization efficiency in rice. Acta Phytoecol Sin (植物生态学报), 2003, 27(4): 466-471 (in Chinese with English abstract)
[19]Feng Y(冯跃), Cao L-Y(曹立勇), Wu W-M(吴伟明), Shen X-H(沈希宏), Zhan X-D(占小登), Zhai R-R(翟荣荣), Chen D-B(陈代波), Cheng S-H(程式华). Conparative analyses of QTLs for N-deficiency tolerance at different seedling stages in rice (Oryza sativa L.). Plant Nutr Fert Sci (植物营养与肥料学报), 2010, 16(4): 880-886 (in Chinese with English abstract)
[20]Zhuang J Y, Lin H X, Lu J, Qian H R, Hittalmani S, Huang N, Zheng K L. Analysis of QTL × environment interaction for yield components and plant height in rice. Theor Appl Genet, 1997, 95: 799-808
[21]Cao G Q, Zhu J, He C X, Gao Y M, Wu P. QTL analysis for epistatic effects and QTL × environment interaction effects on final height of rice (Oryza sativa L.). Acta Genet Sin, 2001, 28(2): 135-143
[22]Li Z-F(李泽福), Zhou T(周彤), Zheng T-Q(郑天清), Luo L-G(罗林广), Xia J-F(夏加发), Zhai H-Q(翟虎渠), Wan J-M(万建民). Analysis of QTL × environment interactions for heading date of rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2002, 28(6): 771-776 (in Chinese with English abstract)
[23]Wang Y(王韵), Cheng L-R(程立锐), Sun Y(孙勇), Zhou Z(周政), Zhu L-H(朱苓华), Xu Z-J(徐正进), Xu J-L(徐建龙). Genetic background effect on QTL expression of heading date and plant height and their interaction with environment in reciprocal introgression lines of rice. Acta Agron Sin (作物学报), 2009, 35(8): 1386-1394 (in Chinese with English abstract)
[24]Lian X M, Xing Y Z, Yan H, Xu C G, Li X H, Zhang Q F. QTLs for nitrogen tolerance at seedling stage identified using a recombinant inbred line population derived from an elite rice hybrid. Theor Appl Genet, 2005, 112: 85-96
[25]Tong H H, Mei H W, Yu X Q, Xu X Y, Li M S, Zhang S Q, Luo L J. Identification of related QTLs at late developmental stage in rice (Oryza sativa L.) under two nitrogen levels. Acta Genet Sin, 2006, 33(5): 458-467
[26]Fang P(方萍), Jing T-W(季天委), Tao Q-N(陶勤南), Wu P(吴平). Detecting QTLs for rice panicle length under two nitrogen levels. Chin J Rice Sci (中国水稻科学), 2002, 16(2): 176-178 (in Chinese with English abstract)
[27]Liu W-J(刘文俊), Wang L-Q(王令强), He Y-Q(何予卿). Comparison of quantitative traits locis controlling plant height and heading date in rice across two related populations. J Huazhong Agric Univ (华中农业大学学报), 2007, 26(2): 161-166 (in Chinese with English abstract)
[28]Xue W Y, Xing Y Z, Weng X Y, Zhao Y, Tang W J, Wang L, Zhou H J, Yu S B, Xu C G, Li X H, Zhang Q F. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40: 761-767
[29]Zhang Y S, Luo L J, Xu C G, Zhang Q F, Xiong Y Z. Quantitative trait loci for panicle size, heading date and plant height co-segregating in trait-performance derived near-isogenic lines of rice(Oryza sativa). Theor Appl Genet, 2006, 113: 361-368
[30]Mu P(穆平), Huang C(黄超), Li J-X(李君霞), Liu L-F(刘立峰), Liu Y-J(刘弋菊), Li Z-C(李自超). Yield trait variation and QTL mapping in a DH population of rice under phosphorus deficiency. Acta Agron Sin (作物学报), 2008, 34(7): 1137-1142 (in Chinese with English abstract)
[31]Chen Q-Q(陈庆全), Yu S-B(余四斌), Li C-H(李春海), Mou T-M(牟同敏). Identification of QTLs for heat tolerance at flowering stage in rice. Sci Agric Sin (中国农业科学), 2008, 41(2): 315-321 (in Chinese with English abstract)
[32]Shen S-Q(沈圣泉), Zhuang J-Y(庄杰云), Shu X-L(舒小丽), Bao J-S(包劲松), Xia Y-W(夏英武). Analysis of QTLs mapping of tolerance to high Al3+ stress at seedling stage in rice. Acta Agron Sin (作物学报), 2006, 32(4): 479-483 (in Chinese with English abstract)
[33]Zhao X-Q(赵秀琴), Xu J-L(徐建龙), Zhu L-H(朱苓华), Li Z-K(黎志康). QTL mapping of yield and root traits under irrigation and drought conditions using advanced backcross introgression lines in rice. Sci Agric Sin (中国农业科学), 2008, 41(7): 1887-1893 (in Chinese with English abstract)
[34]Obara M, Sato T, Sasaki S, Kashiba K, Nagano A, Nakamura I, Ebitani T, Yano M, Yamaya T. Identification and characterization of a QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice. Theor Appl Genet, 2004, 110: 1-11
[35]Shan Y H, Wang Y L, Pan X B. Mapping of QTLs for nitrogen use efficiency and related traits in rice (Oryza sativa L.). Agric Sci China, 2005, 4(10): 721-727
[36]Cho Y, Jiang W Z, Chin J H, Piao Z Z, Cho Y G, Mccouch S R, Koh H J. Identification of QTLs associated with physiological nitrogen use efficiency in rice. Mol Cell, 2007, 23: 72-79
[1] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[2] 郑崇珂, 周冠华, 牛淑琳, 和亚男, 孙伟, 谢先芝. 水稻早衰突变体esl-H5的表型鉴定与基因定位[J]. 作物学报, 2022, 48(6): 1389-1400.
[3] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[4] 郑小龙, 周菁清, 白杨, 邵雅芳, 章林平, 胡培松, 魏祥进. 粳稻不同穗部籽粒的淀粉与垩白品质差异及分子机制[J]. 作物学报, 2022, 48(6): 1425-1436.
[5] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[6] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[7] 杨建昌, 李超卿, 江贻. 稻米氨基酸含量和组分及其调控[J]. 作物学报, 2022, 48(5): 1037-1050.
[8] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[9] 杨德卫, 王勋, 郑星星, 项信权, 崔海涛, 李生平, 唐定中. OsSAMS1在水稻稻瘟病抗性中的功能研究[J]. 作物学报, 2022, 48(5): 1119-1128.
[10] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
[11] 王小雷, 李炜星, 欧阳林娟, 徐杰, 陈小荣, 边建民, 胡丽芳, 彭小松, 贺晓鹏, 傅军如, 周大虎, 贺浩华, 孙晓棠, 朱昌兰. 基于染色体片段置换系群体检测水稻株型性状QTL[J]. 作物学报, 2022, 48(5): 1141-1151.
[12] 王泽, 周钦阳, 刘聪, 穆悦, 郭威, 丁艳锋, 二宫正士. 基于无人机和地面图像的田间水稻冠层参数估测与评价[J]. 作物学报, 2022, 48(5): 1248-1261.
[13] 陈悦, 孙明哲, 贾博为, 冷月, 孙晓丽. 水稻AP2/ERF转录因子参与逆境胁迫应答的分子机制研究进展[J]. 作物学报, 2022, 48(4): 781-790.
[14] 王吕, 崔月贞, 吴玉红, 郝兴顺, 张春辉, 王俊义, 刘怡欣, 李小刚, 秦宇航. 绿肥稻秆协同还田下氮肥减量的增产和培肥短期效应[J]. 作物学报, 2022, 48(4): 952-961.
[15] 巫燕飞, 胡琴, 周棋, 杜雪竹, 盛锋. 水稻延伸因子复合体家族基因鉴定及非生物胁迫诱导表达模式分析[J]. 作物学报, 2022, 48(3): 644-655.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2