欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2011, Vol. 37 ›› Issue (12): 2130-2135.doi: 10.3724/SP.J.1006.2011.02130

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用分子标记定位普通菜豆抗炭疽病基因

陈明丽1,王兰芬1,王晓鸣1,张晓艳2,王述民1,*   

  1. 1江苏沿江地区农业科学研究所, 江苏如皋 226541; 2扬州大学作物遗传生理省级重点实验室, 江苏扬州 225009
  • 收稿日期:2011-04-19 修回日期:2011-06-25 出版日期:2011-12-12 网络出版日期:2011-09-29
  • 通讯作者: 王述民, E-mail: smwang@mail.caas.net.cn
  • 基金资助:

    本研究由国家科技支撑计划项目(2006BAD13B05)和农业部作物种质资源保护与利用专项资助。

Mapping of Gene Conferring Resistance to Anthracnose in Common Bean (Phaseolus vulgaris L.) by Molecular Makers

CHEN Ming-Li1, WANG Lan-Fen1, WANG Xiao-Ming1, ZHANG Xiao-Yan2,WANG Shu-Min1,*   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Qingdao Academy of Agricultural Sciences, Qingdao 266100, China
  • Received:2011-04-19 Revised:2011-06-25 Published:2011-12-12 Published online:2011-09-29
  • Contact: 王述民, E-mail: smwang@mail.caas.net.cn

PDF

1376

被引次数

11

摘要： 为了定位中国普通菜豆的抗炭疽病基因, 选取抗炭疽病地方品种红芸豆(国家库编号F2322)与高感菜豆品种京豆(国家库编号F0777)配制杂交组合, 构建F2抗感分离群体和F2:3家系, 用菜豆炭疽菌81号生理小种鉴定抗病性并分析遗传性。结果表明, 红芸豆对菜豆炭疽菌81号小种的抗性是由一显性单基因控制的, 暂将该基因命名为Co-F2322。用分离群体分组分析法(BSA)和SSR、CAPs分子标记技术, 将该基因定位在B1连锁群上, 利用软件Mapmaker 3.0和Mapchart 3.0计算标记与目的基因间的遗传距离, 检测到3个SSR标记BMc32、C871、Pvm98和2个CAPs标记g1224、g683与抗炭疽病基因连锁, 遗传距离分别为26.06、3.58、13.56、3.81和12.75 cM。

关键词: 玉米自交系, 粗缩病, 抗性鉴定, 遗传多样性

Abstract: Tolocate gene conferring resistance to anthracnose caused by pathogenic fugus (Colletotrichum lindemuthianum) from Chinese common bean, we established F2 plants and F3 families derived from a cross between red common bean cultivar F2322 (resistant to race 81) and Jing common bean cultivar F0777 (susceptible to race 81), identified resistance, and did genetic analysis. The results showed that red bean carried a single dominant gene for resistance to anthracnose, designated as Co-F2322 tentatively. Molecular genetic linkage map was constructed withMapmaker 3.0 and Mapchart 3.0. Three SSR markers (BMc32, C871, Pvm98) and two CAPs makers (g1224 and g683) that linked to the resistance gene were mapped on B1 linkage group of common bean with the distance of 26.06, 3.58, 13.56, 3.81 and 12.75 cM, respectively.

Key words: Common bean, Anthracnose, Bulked segregant analysis (BSA), Anthracnose resistant gene, Molecular marker

[1]Broughton W J, Hernandez G, Blair M, Beebe S, Gepts P, Vanderleyden J. Beans (Phaseolus spp.)—model food legumes. Plant Soil, 2003, 252: 55–128
[2]Wang X-M(王晓鸣), Li Y-L(李怡琳), Li S-Y(李淑英). Study on germplasm of Phaseouus vulgaris L. resistance to Anthracnose. Crop Genet Resour (作物品种资源), 1989, (2): 18–19 (in Chinese)
[3]Rodríguez-Guerra R, Ramírez-Rueda M T, Martínez de la Vega O, Simpson J. Variation in genotype, pathotype and anastomosis groups of Colletotrichum lindemuthianum isolates from Mexico. Plant Pathol, 2003, 52: 228–235
[4]Pastor-Corrales M A. Estandarización de variedades diferenciales y designación de razas de Colletotrichum lindemuthianum. Phytopathology, 1991, 81: 694
[5]Wang K(王坤), Wang X-M(王晓鸣), Zhu Z-D(朱振东), Zhang X-Y(张晓艳), Wang S-M(王述民). Identification of Colletotrichum lindemuthianum races and bean germplasm evaluation for anthracnose resistance. Plant Genet Resour (植物遗传资源学报), 2008, 9(2): 168–172 (in Chinese with English abstract)
[6]Goncalves-Vidigal M C, Vidigal Filho P S, Medeiros A F. Common bean landrace Jalo Listras Pretas is the source of a new Andean anthracnose resistance gene. Crop Sci, 2009, 49: 133–138
[7]Kelly J D, Vallejo V A. A comprehensive review of the major genes conditioning resistance to anthracnose in common bean. Hort Sci, 2004, 39: 1196–1207
[8]Goncalves-Vidigal M C, Lacanallo G F, Vidigal Filho P S. A new gene conferring resistance to anthracnose in common bean (Phaseolus vulgaris L.) cultivar ‘Jalo Vermelho’. Plant Breed, 2008, 127: 592–596
[9]Wu Q-A(吴全安). Identification of crops germplasm resources resistant to diseases and insect pests. Beijing: Agriculture Press, 1991. pp 60–61 (in Chinese)
[10]Afanador L, Hadley S, Kelly J D. Adoption of a mini-prep DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris L.). Annu Rep Bean Improv Coop, 1993, 36: 10–11
[11]Michelmore R M, Paran I, Kesseli R V. Identification of marker linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832
[12]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic maps of experimental and natural population. Genomics, 1987, 1: 174–181
[13]Kosambi D D. The estimation of map distances from recombina-tion values. Ann Eugen, 1944, 12: 172–175
[14]Liu R-H(刘仁虎), Meng J-L(孟金陵). MapDraw: A Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas (遗传), 2003, 25(3): 317–21 (in Chinese with an English abstract)
[15]Wang K(王坤), Wang X-M(王晓鸣), Zhu Z-D(朱振东), Wang S-M(王述民). Mapping of a novel anthracnose resistance gene using SSR markers in common bean (Phaseolus vulgaris L.). Acta Agron Sin (作物学报), 2009, 35(3): 432–437 (in Chinese with English abstract)
[16]Zhao X-Y(赵晓彦), Wang X-M(王晓鸣), Wang S-M(王述民). Identification of anthracnose resistant genes based on SCAR markers in common bean (Phaseolus vulgaris L.). Acta Agron Sin (作物学报), 2007, 33(11): 1815–1821 (in Chinese with English abstract)
[17]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832
[18]Mackay I J, Caligari P D S. Efficiencies of F2 and backcross generations for bulked segregant analysis using dominant markers. Crop Sci, 2000, 40: 626–630
[19]Liao Y(廖毅), Sun B-J(孙保娟), Sun G-W(孙光闻), Liu H-C(刘厚诚), Chen R-Y(陈日远). The application and key problems of bulked segregant analysis on the research of molecular marker in crop. Mol Plant Breed (植物分子育种), 2009, 7(1): 162–168 (in Chinese with English abstract)
[20]Gonçalves-Vidigal M C, Cruz A S, Garcia A. Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND 277. Theor Appl Genet, 2011, 122: 893–903
[1] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[2] 徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 王若若, 王磊, 张立异. 397份高粱种质资源在贵州表型多样性分析及综合评价[J]. 作物学报, 2026, 52(4): 1073-1087.
[3] 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[4] 侯洁, 付朵朵, 武海峰, 郝宇琼, 郑兴卫, 武棒棒, 周凯, 李晓华, 郑军, 赵佳佳. 山西省小麦地方品种的染色体多样性及遗传效应分析[J]. 作物学报, 2026, 52(3): 746-763.
[5] 梅飘, 刘丁丁, 叶圆圆, 张晨禹, 丁诗琦, 李亚奇, 王培鑫, 梅菊芬, 马春雷. 基于茶树液相功能芯片的白化茶树资源遗传多样性分析[J]. 作物学报, 2025, 51(9): 2358-2370.
[6] 王天译, 杨绣娟, 赵佳佳, 郝宇琼, 郑兴卫, 武棒棒, 李晓华, 郝水源, 郑军. 山西小麦醇溶蛋白多样性及其对面粉品质效应研究[J]. 作物学报, 2025, 51(7): 1784-1800.
[7] 王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885.
[8] 肖森林, 阙凡, 周治寰, 张海霞, 邢锦丰, 朱祥彰, 张彦冰, 张楠, 孙轩, 王荣焕, 宋伟, 王维香, 赵久然. 一种玉米穗腐病新病原棘孢木霉的分离与鉴定[J]. 作物学报, 2025, 51(10): 2605-2618.
[9] 马骏, 陈锋, 殷贵鸿, 胡海燕, 魏学宁, 解超杰, 孔令让. 小麦抗茎基腐病遗传育种研究的现状与展望[J]. 作物学报, 2025, 51(10): 2559-2569.
[10] 张红岩, 敏玉霞, 滕长才, 彭小星, 陈志凯, 周仙莉, 娄树宝, 刘玉皎. 利用130K液相芯片分析中国蚕豆种质资源遗传多样性[J]. 作物学报, 2024, 50(8): 1989-2000.
[11] 李长喜, 董占鹏, 关永虎, 刘金伟, 李航, 梅拥军. 南疆陆地棉农艺性状与皮棉产量性状的遗传贡献及决策系数分析[J]. 作物学报, 2024, 50(6): 1486-1502.
[12] 柯会锋, 苏红梅, 孙正文, 谷淇深, 杨君, 王国宁, 徐东永, 王洪这, 吴立强, 张艳, 张桂寅, 马峙英, 王省芬. 棉花现代品种资源产量与纤维品质性状鉴定及分子标记评价[J]. 作物学报, 2024, 50(2): 280-293.
[13] 苏一钧, 赵路宽, 唐芬, 戴习彬, 孙亚伟, 周志林, 刘亚菊, 曹清河. 378份甘薯引进种遗传多样性及群体结构分析[J]. 作物学报, 2023, 49(9): 2582-2593.
[14] 王倩, 张立媛, 许月, 李海, 刘少雄, 薛亚鹏, 陆平, 王瑞云, 刘敏轩. 黍稷高基元EST-SSR标记开发及200份核心种质资源遗传多样性分析[J]. 作物学报, 2023, 49(8): 2308-2318.
[15] 卢茂昂, 彭小爱, 张玲, 汪建来, 何贤芳, 朱玉磊. 基于55K SNP芯片揭示小麦育种亲本遗传多样性[J]. 作物学报, 2023, 49(6): 1708-1714.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2