作物学报 ›› 2012, Vol. 38 ›› Issue (05): 780-790.doi: 10.3724/SP.J.1006.2012.00780
冯万军1,2,李振兴1,2,郭宝健1,2,彭慧茹1,2,姚颖垠1,2,倪中福1,2,*,孙其信1,2,*
FENG Wan-Jun1,2,LI Zhen-Xing1,2,GUO Bao-Jian1,2,PENG Hui-Ru1,2,YAO Ying-Yin1,2,NI Zhong-Fu1,2,*,SUN Qi-Xin1,2,*
摘要: 以耐低磷的小麦基因型洛夫林10号为材料, 采用蛋白质双向电泳技术, 结合质谱鉴定, 分析了正常磷供应和无磷处理7天后根系中的蛋白质组表达谱差异, 以期为深入探讨小麦响应磷胁迫的分子机理提供蛋白水平上的数据和资料。研究发现, 在可重复检测到的1 144个蛋白点中, 有87个在磷胁迫处理前后发生了明显的表达改变,占总数的7.6%,包括磷胁迫前特异表达、磷胁迫后特异表达、磷胁迫后上调和磷胁迫后下调表达等4种差异表达模式。在87个差异蛋白点中,有39个通过质谱技术被成功鉴定,涉及到代谢、细胞生长和分裂、转录和翻译、抗病、信号转导、转座元件及未知功能蛋白等功能类别,说明小麦可能通过细胞的代谢状态和基因表达改变来适应磷胁迫,进而维持体内磷含量的平衡状态。最后,我们还对差异表达点与磷胁迫的关系进行了分析和讨论。
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