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作物学报 ›› 2012, Vol. 38 ›› Issue (05): 904-908.doi: 10.3724/SP.J.1006.2012.00904

• 研究简报 • 上一篇    下一篇

OsBTF3基因在水稻光合作用和生长发育中的功能

李广旭1,2，陈华民1，吴茂森1，何晨阳1,*   

  1. 1 中国农业科学院植物保护研究所 / 植物病虫害生物学国家重点实验室，北京100193；2 辽宁省农业科学院果树研究所，辽宁熊岳115009
  • 收稿日期:2011-09-13 修回日期:2012-01-19 出版日期:2012-05-12 网络出版日期:2012-03-05
  • 通讯作者: 何晨阳, E-mail: cyhe@caas.net.cn, Tel: 010-62894147
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2009ZX08009-044B, 2008ZX08001-002)资助。

Functions of OsBTF3 Gene in Regulation of Photosynthesis, Growth and Development in Rice

LI Guang-Xu1,2,CHEN Hua-Min1,WU Mao-Sen1,HE Chen-Yang1,*   

  1. 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2 Institute of Pomology, Liaoning Academy of Agricultural Sciences, Xiongyue 115009, China
  • Received:2011-09-13 Revised:2012-01-19 Published:2012-05-12 Published online:2012-03-05
  • Contact: 何晨阳, E-mail: cyhe@caas.net.cn, Tel: 010-62894147

PDF

1577

被引次数

9

摘要： 为了揭示转录因子OsBTF3在水稻生长发育中的功能，比较分析了OsBTF3过量表达和RNAi转基因水稻T1代株系叶片生长和株型发育的表型。结果表明，与野生型对照株相比，过量表达株系在叶绿素含量、叶绿体数量、光合速率、叶片大小、株高、节间长方面显著增加或增强; 而RNAi株系的明显降低或减弱。OsBTF3基因的增量或减量表达显著影响水稻光合作用、叶片生长和株型发育。说明OsBTF3在水稻生长发育中具有重要调控功能，可能在水稻转基因分子育种方面具有潜在的应用价值。

关键词: 水稻, OsBTF3, 转基因, 光合作用, 生长发育

Abstract: To reveal the regulatory roles of transcription factor OsBTF3 in growth and development of rice, we comparably analyzed the growth and development of the transgenic rice with over-expressed or RNAi-silenced OsBTF3 gene. Results showed that significant increases in chlorophyll content, chloroplast number, photosynthetic rate, leaf size, plant heights and stem internode length were found in T1 generation of transgenic rice lines with the over-expressed gene compared to wild-type control plants, while remarkable decreases in above phenotypes were observed in plants with the RNAi-silenced gene. Over-expression or silencing of transgene OsBTF3 in rice resulted in significant changes in photosynthesis, growth and development of rice. Therefore, OsBTF3 functions as a key regulator in growth and development, and might be potentially used in molecular breeding of rice for improving agronomic traits.

Key words: Rice, OsBTF3, Transgenic, Photosynthesis, Growth and development

[1]Zheng X M, Moncollin V, Egly J M, Chambon P. A general transcription factor forms a stable complex with RNA polymerase B (II). Cell, 1987, 50: 361–368

[2]Wiedmann B, Sakai H, Davis T A, Wiedmann M. A protein complex required for signal-sequence-specific sorting and translocation. Nature, 1994, 370: 434–440

[3]Lauring B, Kreibich G, Wiedmann M. The intrinsic ability of ribosomes to bind to endoplasmic reticulum membranes is regulated by signal recognition particle and nascent polypeptide-associated complex. Proc Natl Acad Sci USA, 1995, 92: 9435–9439

[4]Yang K S, Kim H S, Jin U H, Lee S S, Park J A, Lim Y P, Pai H S. Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants. Planta, 2007, 225: 1459–1469

[5]Möller I, Beatrix B, Kreibich G, Sakaic H, Lauringa B, Wiedmann M. Unregulated exposure of the ribosomal M-site caused by NAC depletion results in delivery of non-secretory polypeptides to the Sec61 complex. FEBS Lett, 1998, 441: 1–5

[6]Deng J M, Behringer R R. An insertional mutation in the BTF3 transcription factor gene leads to an early postimplantation lethality in mice. Transgenic Res, 1995, 4: 264–269

[7]Bloss T A, Witze E S, Rothman J H. Suppression of CED-3-independent apoptosis by mitochondrial beta-NAC in Caenorhabditis elegans. Nature, 2003, 424: 1066–1071

[8]Cooper B, Hutchison D, Park S, Guimil S, Luginbühl P, Ellero C, Goff S A, Glazbrook J. Identification of rice (Oryza sativa) proteins linked to the cyclin-mediated regulation of the cell cycle. Plant Mol Biol, 2003, 53: 273–279

[9]Freire M A. Translation initiation factor (iso) 4E interacts with BTF3, the β subunit of the nascent polypeptide-associated complex. Gene, 2005, 345: 271–277

[10]Wu M-S(吴茂森), Tan F(田峰), Qi F-J(齐放军), He C-Y(何晨阳). cDNA-AFLP analysis of gene expression response to Xanthomonas oryzae pv. oryzae and identification of genes expressed differentially during this compatible interaction in rice suspension cultured cells. Sci Agric Sin (中国农业科学), 2007, 40(2): 277–282 (in Chinese with English abstract)

[11]Li G-X(李广旭), Wu M-S(吴茂森), Wu J(吴静), He C-Y(何晨阳). Molecular characterization and expression of OsBTF3, a rice gene up-regulated by Xanthomonas oryzae pv. oryzae. Sci Agric Sin (中国农业科学), 2009, 42(7): 2608–2614 (in Chinese with English abstract)

[12]Li G-X(李广旭), Wu M-S(吴茂森), He C-Y(何晨阳). Gene expression response of transcription factor OsBTF3 in rice to bacterial and fungal infection and signal molecule treatment revealed by quantitative real-time PCR analysis. Acta Phytopathol Sin (植物病理学报), 2009, 39(3): 272–277 (in Chinese with English abstract)

[13]Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1989

[14]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-??Ct method. Methods, 2001, 25: 402–408

[15]Arnon D I. Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta vulgaris. Plant Physiol, 1949, 24: 1–15

[16]Sato Y, Sentoku N, Miura Y. Loss-of-function mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants. EMBO J, 1999, 18: 992–1002

[17]Ogi Y. The effects on culm and other agronomic characters caused by semi-dwarfing genes at the sd-locus in rice. Jpn J Breed, 1993, 43: 267–275
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