欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2012, Vol. 38 ›› Issue (06): 1135-1139.doi: 10.3724/SP.J.1006.2012.01135

• 研究简报 • 上一篇    

利用SSR分子标记分析彩色小麦的亲缘关系与遗传多样性

黄玮，杨敏，秦保平，王振林，武玉国，孙兰珍，尹燕枰   

  1. 作物生物学国家重点实验室 / 山东省作物生物学重点实验室 / 山东农业大学农学院, 山东泰安  271018
  • 收稿日期:2011-11-28 修回日期:2012-02-22 出版日期:2012-06-12 网络出版日期:2012-04-06
  • 通讯作者: 尹燕枰, E-mail: ypyinsdau@sina.com, Tel: 0538-8242458
  • 基金资助:

    本研究由公益性行业(农业)科研专项(201203029)，国家转基因生物新品种培育重大专项(2009ZX08002-004B)和国家重点基础研究计划(973计划)项目(2009CB118602)资助。

Relationships and Genetic Diversity of Colored-Grain Wheat Detected by SSR Markers

HUANG Wei,YANG Min,QIN Bao-Ping,WANG Zhen-Lin,WU Yu-Guo,SUN Lan-Zhen,YIN Yan-Ping*   

  1. State Key Laboratory of Crop Biology / Shandong Provincial Key Laboratory of Crop Biology / Agronomy College of Shandong Agricultural University, Tai’an 271018, China
  • Received:2011-11-28 Revised:2012-02-22 Published:2012-06-12 Published online:2012-04-06
  • Contact: 尹燕枰, E-mail: ypyinsdau@sina.com, Tel: 0538-8242458

PDF

1248

被引次数

25

摘要： 为探讨彩色小麦的亲缘关系和遗传多样性, 利用305对多态性SSR引物检测了18个彩色小麦品种(系)。共检测到1 084个等位变异, 平均每对引物检测到3.6个。红粒、紫红粒小麦的等位变异数在7个部分同源群的排序为HG3>HG2>HG1>HG4>HG5>HG7>HG6, 黑粒和蓝粒小麦的排序为HG3>HG2>HG1和HG4>HG5>HG7>HG6。不同粒色小麦的SSR标记多态信息含量相近, 其中红粒和紫红粒小麦为0.525~0.543, 黑粒和蓝粒小麦为0.517~0.545。18个品种(系)的遗传差异极显著(P=0.0001), 遗传相似系数在0.41~0.76之间。UPGMA聚类分析将18份材料分为6群,类群分布与亲缘关系及其地域相关。

关键词: 彩色小麦, SSR标记, 遗传多样性

Abstract: To reveal the relationship and genetic diversity of colored-grain wheat, we analyzed 18 varieties (lines) with red, purple, blue, and black seed coats using 305 polymorphic SSR markers. A total of 1084 alleles were detected with 3.6 alleles per locus. The allele numbers in seven homoeologous groups (HG) were aligned as HG3>HG2>HG1>HG4>HG5>HG7>HG6 in red and purple wheat and HG3>HG2>HG1 and HG 4>HG5>HG7>HG6 in black and blue wheat. The polymorphic information content (PIC) values in the seven homoeologous groups were similar, which were 0.525–0.543 in red and purple wheat and 0.517–0.545 in black and blue wheat. Among the 18 colored-grain varieties (lines), genetic difference was significant (P = 0.0001), and the genetic similarity coefficients ranged from 0.41 to 0.76. According to UPGMA analysis, the 18 varieties (lines) were classified into six groups, which were in relation to variety kindred and geographic distance.

Key words: Colored-grain wheat, SSR markers, Genetic diversity

[1]Zhou G-J(周桂娟), Wang K(王坤), Liu J-M(刘佳明), Sun H-J(孙慧君), Li J-L(李集临), Zhang J(张杰). The pigment color wheat spectroscopic and antioxidant properties. Chin Agric Sci Bull(中国农学通报), 2011, 27(5): 109-114 (in Chinese with English abstract)

[2]Ma D(马东), Yin Y-P(尹燕枰), Sun L-Z(孙兰珍), Wu Y-H(邬云海), Tang X-Z(唐晓珍), Li J-M(李建敏), Wang Z-L(王振林). Comparison of protein and mineral nutrition contents of different milling streams between black and white wheat cultivars. Science Paper Online (中国科技论文在线).[2011-9-11] http://www.paper.edu.cn (in Chinese with English abstract)

[3]Tang X-Z(唐晓珍), Liu B(刘宾), Jiang Y(姜媛), Sun L-Z(孙兰珍), Yin Y-P(尹燕枰). Technological conditions for extraction of pigment from purple wheat bran by acidified alcohol. J Chin Cereals Oils Assoc (中国粮油学报), 2008, 23(5): 24-27 (in Chinese with English abstract)

[4]Wang Y-Y(王耀勇), Wang C-Y(王长有), Ji W-Q(吉万全), Wang Q-H(王秋会). Pigment’s stability in grains of wheat with blue grain or violet grain. J Triticeae Crops (麦类作物学报), 2008, 28(2) :316-320 (in Chinese with English abstract)

[5]Tang X-Z(唐晓珍), Wang Z(王征), Zhang X-S(张宪省), Wang X-D(王晓东), Sun L-Z(孙兰珍), Yin Y-P(尹燕枰). Red and black Tai’an wheat lines: nutritional quality evaluation. J Chin Cereals Oils Assoc (中国粮油学报), 2009, 24(4): 28-31 (in Chinese with English abstract)

[6]Wang H-W(王海伟), Wang Z-L, Wang P (王平), Wang S-G(王树刚), Huang W(黄玮), Wu Y-G(武玉国), Sun L-Z(孙兰珍), Yin Y-P(尹燕枰). Effect of shading post anthesis on anthocyanin accumulation and activities of related enzymes in colored-grain wheat. Acta Agron Sin (作物学报), 2011, 37(6): 1093−1100 (in Chinese with English abstract)

[7]Sun Q(孙群), Sun B-Q(孙宝启), Wang J-H(王建华). Genetic of seed pigment of black kernel wheat. Seed (种子), 2003, (4): 33-34 (in Chinese with English abstract)

[8]Lan S-Q(兰素缺), Li X-P(李杏普), Liu Y-P(刘玉平). Genetic of seed pigment of blue kernel wheat. Acta Agric Boreali-Sin (华北农学报), 2008, 23(suppl-1): 12-14 (in Chinese with English abstract)

[9]Liu C(刘成), Yang Z-J(杨足君), Feng J(冯娟), Zhou J-P(周建平), Chi S-H(迟世华), Ren Z-L(任正隆). Development of Dasypyrum genome specific marker by using wheat microsatellites. Hereditas (遗传), 2006, 28(12): 1573-1579 (in Chinese with English abstract)

[10]Li X-J(李学军), Pan Y-M(潘玉朋), Wang X-L(王小利), Li L-Q(李立群), Wang P(王培), Feng Y(冯毅), Wang H(王辉). Evolution of genetic diversity of the wheat varieties grown at Shananxi. J Northwest A&F Univ (西北农林科技大学学报), 2011, 39(4): 48-54 (in Chinese with English abstract)

[11]Pan Y-P(潘玉朋), Li L-Q(李立群), Zheng J-J(郑锦娟), Wang P(王培), Feng Y(冯毅), Li X-J(李学军). Analysis of genetic diversity of the large-scale promoted wheat varieties grown at Huang-Huai area in recent years. Acta Agric Boreali-Occid Sin (西北农业学报), 2011, 20(4): 47-52 (in Chinese with English abstract)

[12]Li J-Z(李建钊), Li Z-H(李志辉). Analysis of developed varieties of black wheat. China Seed Ind (中国种业), 2007, (10): 17-19 (in Chinese)

[13]Han L-M(韩利明), Zhang Y(张勇), Peng H-R(彭惠茹), Qiao W-C(乔文臣), He M-Q(何明琦), Wang H-G(王洪刚), Qu Y-Y(曲延英), Liu C-L(刘春来), He Z-H(何中虎). Analysis of heat resistance for cultivars from north China winter wheat region by yield and quality traits. Acta Agron Sin (作物学报), 2010, 36(9): 1538-1546 (in Chinese with English abstract)

[14]Zhao Y(赵琰), Qin L-Y(秦丽燕), Yang W(杨伟), Liang R-Q(梁荣奇), You M-S(尤明山), Liu G-T(刘广田). Analysis on distribution of grain iron content in 83 common wheat varieties. Chin Agric Sci Bull(中国农学通报), 2010, 26(23): 126-129 (in Chinese with English abstract)

[15]Wang Q-Y(王秋叶), Zhang J-C(张建诚), Guo Z-Y(郭志远), Yao J-Z(姚景珍). Breeding and characteristics of Wumai 526. Crop Germplasm Resour (作物品种资源), 1998, (3): 22-24 (in Chinese)

[16]Zhang G-W(张国武), Zhou Y-Z(周彦中), Zhang C-Y(张春映), Li J-Z(李建钊). Breeding and characteristics of black wheat Luozhen 1. Henan Agric Sci (河南农业科学), 1997, (8): 10 (in Chinese)

[17]Song W(宋伟), Sun L-Z(孙兰珍), Zhao A-H(赵爱红), Liu Q(刘青). Nutrition qualitative analysis and verification of colorful wheat variant offspring produced by exogenous DNA introduction. Acta Bot Borea1i-Occident Sin (西北植物学报), 2004, 24(6): 966-970 (in Chinese with English abstract)

[18]Zhou H-P(周汉平), Li B(李壁), Li Z-S(李振声). The study of breeding blue-grain translocation of wheat. Acta Bot Borea1i-Occident Sin (西北植物学报), 1995, 15(2): 125-128 (in Chinese with English abstract) 

[19]Ying J(英加), Li B(李滨), Mu S-M(穆素梅), Zhou H-P(周汉平), Liu J-Z(刘建中), Li Z-S(李振声). Identification of blue-grained wheat translocation lines using fluorescence in situ hybridization. Acta Bot Sin (植物学报), 2001, 43(2): 164-168 (in Chinese with English abstract)
[1] 肖颖妮, 于永涛, 谢利华, 祁喜涛, 李春艳, 文天祥, 李高科, 胡建广. 基于SNP标记揭示中国鲜食玉米品种的遗传多样性[J]. 作物学报, 2022, 48(6): 1301-1311.
[2] 王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.
[3] 韩贝, 王旭文, 李保奇, 余渝, 田琴, 杨细燕. 陆地棉种质资源抗旱性状的关联分析[J]. 作物学报, 2021, 47(3): 438-450.
[4] 刘少荣, 杨扬, 田红丽, 易红梅, 王璐, 康定明, 范亚明, 任洁, 江彬, 葛建镕, 成广雷, 王凤格. 基于农艺及品质性状与SSR标记的青贮玉米品种遗传多样性分析[J]. 作物学报, 2021, 47(12): 2362-2370.
[5] 孙倩, 邹枚伶, 张辰笈, 江思容, Eder Jorge de Oliveira, 张圣奎, 夏志强, 王文泉, 李有志. 基于SNP和InDel标记的巴西木薯遗传多样性与群体遗传结构分析[J]. 作物学报, 2021, 47(1): 42-49.
[6] 赵孟良,王丽慧,任延靖,孙雪梅,侯志强,杨世鹏,李莉,钟启文. 257份菊芋种质资源表型性状的遗传多样性[J]. 作物学报, 2020, 46(5): 712-724.
[7] 张红岩,杨涛,刘荣,晋芳,张力科,于海天,胡锦国,杨峰,王栋,何玉华,宗绪晓. 利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性[J]. 作物学报, 2020, 46(3): 330-340.
[8] 刘易科,朱展望,陈泠,邹娟,佟汉文,朱光,何伟杰,张宇庆,高春保. 基于SNP标记揭示我国小麦品种(系)的遗传多样性[J]. 作物学报, 2020, 46(02): 307-314.
[9] 叶卫军,陈圣男,杨勇,张丽亚,田东丰,张磊,周斌. 绿豆SSR标记的开发及遗传多样性分析[J]. 作物学报, 2019, 45(8): 1176-1188.
[10] 吴迷,汪念,沈超,黄聪,温天旺,林忠旭. 基于重测序的陆地棉InDel标记开发与评价[J]. 作物学报, 2019, 45(2): 196-203.
[11] 卢媛,艾为大,韩晴,王义发,李宏杨,瞿玉玑,施标,沈雪芳. 糯玉米自交系SSR标记遗传多样性及群体遗传结构分析[J]. 作物学报, 2019, 45(2): 214-224.
[12] 陈芳,乔麟轶,李锐,刘成,李欣,郭慧娟,张树伟,常利芳,李东方,阎晓涛,任永康,张晓军,畅志坚. 小麦新种质CH1357抗白粉病遗传分析及染色体定位[J]. 作物学报, 2019, 45(10): 1503-1510.
[13] 薛延桃,陆平,史梦莎,孙昊月,刘敏轩,王瑞云. 新疆、甘肃黍稷资源的遗传多样性与群体遗传结构研究[J]. 作物学报, 2019, 45(10): 1511-1521.
[14] 刘洪,徐振江,饶得花,鲁清,李少雄,刘海燕,陈小平,梁炫强,洪彦彬. 基于形态学性状和SSR标记的花生品种遗传多样性分析和特异性鉴定[J]. 作物学报, 2019, 45(1): 26-36.
[15] 黄聪,李晓方,李定国,林忠旭. 利用陆地棉MAGIC群体定位产量、生育期和株高性状的QTL[J]. 作物学报, 2018, 44(9): 1320-1333.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2