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作物学报 ›› 2012, Vol. 38 ›› Issue (08): 1354-1360.doi: 10.3724/SP.J.1006.2012.01354

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦TaPHYA基因亚家族的克隆及表达分析

王霞1,2,**，马燕斌2,3,4,**，宋梅芳2，孟凡华2，李秀全2，杨丽2，吴霞4，杨克诚3,*，杨建平2,*   

  1. 1山西运城学院生命科学系，山西运城 044000；2中国农业科学院作物科学研究所，北京100081；3四川农业大学玉米研究所，四川雅安625014；
    4山西省农业科学院棉花研究所，山西运城 044000
  • 收稿日期:2012-03-08 修回日期:2012-04-20 出版日期:2012-08-12 网络出版日期:2012-06-05
  • 通讯作者: 杨建平, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859; 杨克诚, Tel: 0835-2882465
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2011ZX08010-002), 国家重点基础研究发展计划(973计划)项目(2009CB118300)和国家高技术研究发展计划(863计划)项目(2008AA10Z121)资助。

Isolation and Expression Patterns of TaPHYA Gene Subfamily in Common Wheat

WANG Xia1,2,**,MA Yan-Bin2,3,4,**,MENG Fan-Hua2,LI Xiu-Quan2,YANG Li2,Wu-Xia4,YANG Ke-Cheng3,*,YANG Jian-Ping2 ,*   

  1. 1 Department of Life Science, Yuncheng University, Yuncheng 044000, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Maize Research Institute, Sichuan Agricultural University, Ya’an 450002, China; 4 Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China
  • Received:2012-03-08 Revised:2012-04-20 Published:2012-08-12 Published online:2012-06-05
  • Contact: 杨建平, E-mail: yangjianping@caas.net.cn, Tel: 010-82105859; 杨克诚, Tel: 0835-2882465

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被引次数

5

摘要： 光敏色素是一类与作物农艺性状密切相关的基因家族。本研究克隆了中国春小麦光敏色素TaPHYA1、TaPHYA2和TaPHYA3基因完整的编码序列，并对它们进行了生物信息学以及转录分析。在NCBI数据库中对其推测的氨基酸序列进行BLAST分析，发现TaPHYA1和TaPHYA3氨基酸序列中包含光敏色素基因完整的功能结构域。系统进化树分析表明，小麦TaPHYA1、TaPHYA2和TaPHYA3之间进化关系相近，且与单子叶植物玉米、水稻的PHYA聚为一个亚类。另外，TaPHYA的表达丰度具有组织特异性，在茎、叶、穗中表达量分别是根中的1.35、0.34和0.87倍；而在同光质条件下，其表达丰度也具有光质特异性，TaPHYA的总表达量在黑暗和远红光条件下最高，在蓝光条件下次之，而红光和白光条件下最低。该结果为深入研究小麦光敏色素A基因亚家族的功能奠定了基础。

关键词: 小麦, 光敏色素A, 组织特异性, 表达分析

Abstract: Phytochromes (PHYs) are important genes relating to crop agronomic traits. In this paper, TaPHYA1, TaPHYA2, and TaPHYA3 were cloned fromcv. Chinese Spring(Triticum aestivum L.). Theputative domains ofTaPHYA1, TaPHYA2, and TaPHYA3 proteins were predicted via the NCBI Protein BLAST. Either TaPHYA1 or TaPHYA3 was composed of a GAF domain, a PHYtochrome domain, two PAS domains, a His Kinase A domain, and a Histidine kinase-like ATPase domain.Phylogenetic tree analysis indicated that TaPHYA1, TaPHYA2, and TaPHYA3 were closer to PHYA members of monocot plants (ZmPHYA, SbPHYA, and OsPHYA) rather than to those of dicot plants (AtPHYA and GmPHYA). Expression levels of TaPHYAs were analyzed using semi quantitative RT-PCR and real time PCR assays.Tissue-specific expression of total TaPHYA was also detected in stem, leaf, and spike,whose levelswere 1.35, 0.34, and 0.87 times of that in root. Additionally, TaPHYA showed high expression level in darkness, far-red, and blue light conditions, but low expression level in red and white light conditions. The transcription level of TaPHYA in the seedlings grown in the darkness or under far-red light was four or three times of that in seedlings grown in red light, respectively.

Key words: Triticum aestivum, Phytochrome A, Tissue-specific expression, Transcription expression analysis

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