欢迎访问作物学报,今天是

作物学报 ›› 2013, Vol. 39 ›› Issue (01): 21-28.doi: 10.3724/SP.J.1006.2013.00021

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

陆地棉背景下海岛棉第18染色体片段置换系的培育及相关农艺性状QTL定位

付央,苑冬冬,胡文静,蔡彩平,郭旺珍*   

  1. 南京农业大学 / 作物遗传与种质创新国家重点实验室 / 教育部杂交棉创制工程研究中心, 江苏南京210095
  • 收稿日期:2012-05-02 修回日期:2012-09-05 出版日期:2013-01-12 网络出版日期:2012-11-15
  • 通讯作者: 郭旺珍, E-mail: moelab@njau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金重点项目(30730067)和江苏省“333工程”人才项目资助。

Development of Gossypium barbadense Chromosome 18 Segment Substitution Lines in the Genetic Standard Line TM-1 of Gossypium hirsutum and Mapping of QTLs Related to Agronomic Traits

FU Yang,YUAN Dong-Dong,HU Wen-Jing,CAI Cai-Ping,GUO Wang-Zhen*   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-05-02 Revised:2012-09-05 Published:2013-01-12 Published online:2012-11-15
  • Contact: 郭旺珍, E-mail: moelab@njau.edu.cn

摘要:

Sub18是以陆地棉遗传标准系TM-1为背景, 含海岛棉3-79第18染色体的置换系材料。本研究以TM-1为受体亲本, 置换系Sub18为供体亲本, 借助分子标记辅助选择技术培育了一套以TM-1为背景, 含海岛棉3-79第18染色体不同长度片段的置换系。这套置换系由45个株系构成, 共78个置换片段。其中27个株系导入单片段, 占总株系的60%; 9个株系导入2个片段, 占20%; 9个株系导入3个及以上片段, 占20%。导入片段总长度为467.6 cM, 约为该染色体遗传长度的4倍, 每个株系内被替换的染色体片段长度不完全相同, 平均遗传长度为5.99 cM, 最短的为0.9 cM, 最长的20.35 cM。其中13个株系表现开放花蕾性状, 涉及的最短导入片段长5.05 cM。对TM-1、Sub18以及培育的45个导入系进行农艺性状调查和QTL联合定位分析, 鉴定出纤维强度(qFS-C18-1)、整齐度(qFU-C18-1)、马克隆值(qFMi-C18-1)、成熟度(qFMa-C18-1)、皮棉重(qLW-C18-1)、籽指 (qSI-C18-1)和衣分 (qLP-C18-1) 7个加性QTL和5个上位性效应QTL。研究结果为进一步精细定位目标QTL、克隆QTL以及重要性状分子设计育种奠定了基础。

关键词: 染色体片段置换系, 分子标记, QTL, TM-1, Sub18, 开放花蕾

Abstract:

Substitution line 18 (Sub18) has the background of genetic standard line of G. hirsutum acc. TM-1 with the 18th chromosome of G. barbadense accession 3-79. In the study, a set of G. barbadense accession 3-79 chromosome 18 segment substitution lines (CSSL18) were developed via molecular marker-assisted selection (MAS), with TM-1 as a recipient parent and Sub18 as a donor. The developed CSSLs consisted of 45 lines and 78 introgressed segments in total. Of them, 27 were introgressed with single segment, accounting for 60% of the total lines; 9 were introgressed with two segments, accounting for 20%; and 9 were introgressed with three or more segments, accounting for 20%. The total length of introgression segments was 467.6 cM, which is 4 times of the genetic length of the chromosome 18. The substituted segment length varied in each line, ranging from the shortest of 0.9 cM to the longest of 20.35 cM, with an average length of 5.99 cM. Further, the opening bud trait was detected in 13 substitution lines, with the shortest introgressed segment of 5.05 cM. Using TM-1, Sub18 and 45 substituted lines as materials, we investigated agronomic traits and carried out QTL tagging by joint positioning analysis. Seven additive effect QTLs for fiber strength (qFS-C18-1), uniformity (qFU-C18-1), micronaire (qFMi-C18-1), maturity (qFMa-C18-1), lint weight (qLW-C18-1), seed index (qSI-C18-1), and lint percentage (qLP-C18-1) and five epistatic effect QTLs were identified, respectively. The results will lay a foundation for the fine mapping and cloning of target QTLs, and the molecular breeding by design for pyramiding multi-traits in cotton.

Key words: Chromosome segment substitution lines, Molecular marker, QTL, TM-1, Sub18, Opening bud

[1]Paterson A H, Deverna J W, Lanini B, Tanksley S D. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato. Genetics, 1990, 124: 735–742



[2]Eshed Y, Zamir D. An introgression line population of lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics, 1995, 141: 1147–1162



[3]Yamamoto T, Kuboki Y, Lin S Y, Sasaki T, Yano M. Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors. Theor Appl Genet, 1998, 97: 37–44



[4]Liu G-M(刘冠明), Li W-T(李文涛), Zeng R-Z(曾瑞珍), Zhang G-Q(张桂权). Development of single segment substitution lines (SSSLs) of subspecies in rice. Chin Rice Sci (中国水稻科学), 2003, 17(3): 201–204 (in Chinese with English abstract)



[5]Pan J-J(潘家驹). Cotton Breeding (棉花育种学). Beijing: China Agriculture Press, 1998. pp 60–210 (in Chinese)



[6]Kohel R J, Endrizzi J E, White T G. An evaluation of Gossypium barbadense L. chromosome 6 and 17 in the G. hirsutum L. genome. Crop Sci, 1977, 17: 404–406



[7]Ma J-Z(马家璋), Kohe R J. An evaluation of six substituton lines of Gossypium barbadense chromosome in G. hirsutum. Acta Agron Sin (作物学报), 1983, 9(3): 145–150 (in Chinese with English abstract)



[8]Ren L H, Guo W Z, Zhang T Z. Identification of QTLs affecting yield and fiber properties in chromosome 16 in cotton using substitution line. Acta Bot Sin, 2002, 44(7): 815–820



[9]Wang P, Ding Y Z, Lu Q X, Guo W Z, Zhang T Z. Development of Gossypium barbadense chromosome segment substitution lines in the genetic standard line TM-1 of Gossypium hirsutum. Chin Sci Bull, 2008, 53(10): 1512–1517



[10]Wang P, Zhu Y J, Song X L, Cao Z B, Ding Y Z, Liu B L, Zhu X F, Wang S, Guo W Z, Zhang T Z. Inheritance of long staple fiber quality traits of Gossypium barbadense in G. hirsutum background using CSILs. Theor Appl Genet, 2012, 124: 1415–1428



[11]Guo W Z, Cai C P, Wang C B, Zhao L, Wang L, Zhang T Z. A preliminary analysis of genome structure and composition in Gossypium hirsutum. BMC Genomics, 2008, 9: 314



[12]Young N D, Tanksley S D. Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet, 1989, 77: 95–101



[13]Wang J K, Wan X Y, Crossa J, Crouch J, Weng J F, Zhan H Q, Wan J M. QTL mapping of grain length in rice (Oryza sativa L.) using chromosome segment substitution lines. Genet Res, 2006, 88:93-104



[14]Li H H, Ribaut J M, Li Z L, Wang J K. Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations. Theor Appl Genet, 2008, 116: 243–260



[15]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11–13



[16]Zhu Y-J(朱亚娟), Wang P(王鹏), Guo W-Z(郭旺珍), Zhang T-Z (张天真). Mapping QTLs for lint percentage and seed index using Gossypium barbadense chromosome segment introgression lines. Acta Agron Sin (作物学报), 2010, 36(8): 1318–1323 (in Chinese with English abstract)



[17]Endrizzi J E. Linkage analysis of open bud and yellow petal (Y1) in cotton. Genome, 1991, 34: 461–63



[18]Qian N, Zhang X W, Guo W Z, Zhang T Z. Fine mapping of open-bud duplicate genes in homoelogous chromosomes of tetraploid cotton. Euphytica, 2009, 165: 325–331



[19]Liao C Y, Wu P, Hu B, Yi K K. Effects of genetic background and environment on QTLs and epistasis for rice (Oryza sativa L. ) panicle number. Theor Appl Genet, 2001, 103: 104–111



[20]Eshed Y, Zamir D. Less-than-additive epistatic interactions of quantitative trait loci in tomato. Geneties, 1996, 143: 1807–1817



[21]Lin Z-X(林忠旭), Feng C-H(冯常辉), Guo X-P(郭小平), Zhang X-L(张献龙). Genetic analysis of major QTLs and epistasis interaction for yield and fiber quality in upland cotton. Sci Agri Sin (中国农业科学), 2009, 42(9): 3036–3047 (in Chinese with English abstract)



[22]Wang J K, Wan X Y, Li H H, Pfeiffer W H, Crouch J , Wan J M. Application of identified QTL-marker associations in rice quality improvement through a design-breeding approach. Theor Appl Genet, 2007, 115: 87–100



[23]Wang Z-Q(王智权), Liu X(刘喜), Jiang L(江玲), Yang C(杨超), Liu S-J(刘世家), Chen L-M(陈亮明), Zhan H-Q(翟虎渠), Wan J-M(万建民). QTL detection for flag leaf morphological traits of rice in a population of chromosome segment substitution lines. J Nanjing Agri Univ (南京农业大学学报), 2010, 33(6): 1–6 (in Chinese with English abstract)



[24]Ou-Yang L(欧阳恋). Identification, mapping and pyramiding of genes for grain quality based on SSSLs. MS thesis of South China Agricultural University, 2006 (in Chinese with English abstract)



[25]Huang Y-F(黄益峰). Identification, pyramiding and epistasis analysis of the rice grain shape and grain weight QTL. MS thesis of South China Agricultural University, 2006 (in Chinese with English abstract)

[1] 翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603.
[2] 彭佳泺, 李颖, 李丹丹, 杨军宁, 郭学峰, 张文姣, 俞晓雪, 周亚荣, 王振玉, 王彩香, 马雄风, 宿俊吉. 陆地棉I类LBD家族成员鉴定及GhLBD6调控开花期的功能和单倍型分析[J]. 作物学报, 2026, 52(6): 1682-1697.
[3] 郑玉珍, 齐飞艳, 孙子淇, 刘华, 秦利, 石磊, 王娟, 汪蒙蒙, 韩锁义, 徐静, 苗利娟, 黄冰艳, 董文召, 郑峥, 张新友. 花生籽仁总超长链脂肪酸和7种脂肪酸组分的QTL定位[J]. 作物学报, 2026, 52(6): 1646-1657.
[4] 张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727.
[5] 刘长友, 王珅, 时会影, 沈颖超, 孙蕾, 王彦, 张志肖, 苏秋竹, 田静, 范保杰. 基于饭豆基因资源的小豆远缘杂交群体抗豆象QTL定位[J]. 作物学报, 2026, 52(3): 936-944.
[6] 王楚锐, 李开祥, 赵志, 肖麓, 唐国永, 赵志刚, 徐亮, 杜德志, 柳海东. 甘蓝型春油菜早花基因BnCRY2功能位点KASP标记的开发及应用[J]. 作物学报, 2026, 52(3): 708-721.
[7] 王粤生, 葛冬冬, 程兰斐, 陈春环, 王长有, 刘新伦, 李停栋, 邓平川, 吉万全, 赵继新. 小麦-华山新麦草二体异代换系16DH25-7的分子细胞遗传学及抗病性鉴定[J]. 作物学报, 2026, 52(2): 433-445.
[8] 张飞飞, 何万龙, 焦文娟, 白斌, 耿洪伟, 程宇坤. 小麦抗条锈病相关性状元分析及候选基因分析[J]. 作物学报, 2025, 51(8): 2111-2127.
[9] 胡蒙, 沙丹, 张晟瑞, 谷勇哲, 张世碧, 李静, 孙君明, 邱丽娟, 李斌. 大豆分枝数QTL定位及候选基因筛选[J]. 作物学报, 2025, 51(7): 1747-1756.
[10] 邵顺伟, 陈卓, 兰振东, 蔡兴奎, 邹华芬, 李晨曦, 唐景华, 朱熙, 张彧, 董建科, 金辉, 宋波涛. 基于BSA-seq技术的块茎芽眼深度QTL定位分析[J]. 作物学报, 2025, 51(7): 1725-1735.
[11] 杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724.
[12] 郭栋财, 吕涛, 蔡永生, 买吾鲁达·艾合买提, 全家, 曲延英, 郑凯. 棉花纤维品质相关性状QTL元分析及候选基因鉴定[J]. 作物学报, 2025, 51(6): 1445-1466.
[13] 张金泽, 周庆国, 肖莉晶, 金海润, 欧阳青静, 龙旭, 晏中彬, 田恩堂. 芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析[J]. 作物学报, 2025, 51(5): 1166-1177.
[14] 林伟津, 郭泽佳, 刘浩, 李海芬, 王润风, 黄璐, 余倩霞, 陈小平, 洪彦彬, 李少雄, 鲁清. 花生荚果产量相关性状QTL定位与候选基因分析[J]. 作物学报, 2025, 51(4): 969-981.
[15] 展宗冰, 靳奇峰, 刘迪, 吕迎春, 郭莹, 张雪婷, 虎梦霞, 王尚, 杨芳萍. 甘肃省小麦农家种老芒麦分子鉴定及其重要性状评价[J]. 作物学报, 2025, 51(3): 609-620.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!