作物学报 ›› 2013, Vol. 39 ›› Issue (01): 21-28.doi: 10.3724/SP.J.1006.2013.00021
付央,苑冬冬,胡文静,蔡彩平,郭旺珍*
FU Yang,YUAN Dong-Dong,HU Wen-Jing,CAI Cai-Ping,GUO Wang-Zhen*
摘要:
Sub18是以陆地棉遗传标准系TM-1为背景, 含海岛棉3-79第18染色体的置换系材料。本研究以TM-1为受体亲本, 置换系Sub18为供体亲本, 借助分子标记辅助选择技术培育了一套以TM-1为背景, 含海岛棉3-79第18染色体不同长度片段的置换系。这套置换系由45个株系构成, 共78个置换片段。其中27个株系导入单片段, 占总株系的60%; 9个株系导入2个片段, 占20%; 9个株系导入3个及以上片段, 占20%。导入片段总长度为467.6 cM, 约为该染色体遗传长度的4倍, 每个株系内被替换的染色体片段长度不完全相同, 平均遗传长度为5.99 cM, 最短的为0.9 cM, 最长的20.35 cM。其中13个株系表现开放花蕾性状, 涉及的最短导入片段长5.05 cM。对TM-1、Sub18以及培育的45个导入系进行农艺性状调查和QTL联合定位分析, 鉴定出纤维强度(qFS-C18-1)、整齐度(qFU-C18-1)、马克隆值(qFMi-C18-1)、成熟度(qFMa-C18-1)、皮棉重(qLW-C18-1)、籽指 (qSI-C18-1)和衣分 (qLP-C18-1) 7个加性QTL和5个上位性效应QTL。研究结果为进一步精细定位目标QTL、克隆QTL以及重要性状分子设计育种奠定了基础。
[1]Paterson A H, Deverna J W, Lanini B, Tanksley S D. Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes in an interspecies cross of tomato. Genetics, 1990, 124: 735–742[2]Eshed Y, Zamir D. An introgression line population of lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics, 1995, 141: 1147–1162[3]Yamamoto T, Kuboki Y, Lin S Y, Sasaki T, Yano M. Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors. Theor Appl Genet, 1998, 97: 37–44[4]Liu G-M(刘冠明), Li W-T(李文涛), Zeng R-Z(曾瑞珍), Zhang G-Q(张桂权). Development of single segment substitution lines (SSSLs) of subspecies in rice. Chin Rice Sci (中国水稻科学), 2003, 17(3): 201–204 (in Chinese with English abstract)[5]Pan J-J(潘家驹). Cotton Breeding (棉花育种学). Beijing: China Agriculture Press, 1998. pp 60–210 (in Chinese)[6]Kohel R J, Endrizzi J E, White T G. An evaluation of Gossypium barbadense L. chromosome 6 and 17 in the G. hirsutum L. genome. Crop Sci, 1977, 17: 404–406[7]Ma J-Z(马家璋), Kohe R J. An evaluation of six substituton lines of Gossypium barbadense chromosome in G. hirsutum. Acta Agron Sin (作物学报), 1983, 9(3): 145–150 (in Chinese with English abstract)[8]Ren L H, Guo W Z, Zhang T Z. Identification of QTLs affecting yield and fiber properties in chromosome 16 in cotton using substitution line. Acta Bot Sin, 2002, 44(7): 815–820[9]Wang P, Ding Y Z, Lu Q X, Guo W Z, Zhang T Z. Development of Gossypium barbadense chromosome segment substitution lines in the genetic standard line TM-1 of Gossypium hirsutum. Chin Sci Bull, 2008, 53(10): 1512–1517[10]Wang P, Zhu Y J, Song X L, Cao Z B, Ding Y Z, Liu B L, Zhu X F, Wang S, Guo W Z, Zhang T Z. Inheritance of long staple fiber quality traits of Gossypium barbadense in G. hirsutum background using CSILs. Theor Appl Genet, 2012, 124: 1415–1428[11]Guo W Z, Cai C P, Wang C B, Zhao L, Wang L, Zhang T Z. A preliminary analysis of genome structure and composition in Gossypium hirsutum. BMC Genomics, 2008, 9: 314[12]Young N D, Tanksley S D. Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet, 1989, 77: 95–101[13]Wang J K, Wan X Y, Crossa J, Crouch J, Weng J F, Zhan H Q, Wan J M. QTL mapping of grain length in rice (Oryza sativa L.) using chromosome segment substitution lines. Genet Res, 2006, 88:93-104[14]Li H H, Ribaut J M, Li Z L, Wang J K. Inclusive composite interval mapping (ICIM) for digenic epistasis of quantitative traits in biparental populations. Theor Appl Genet, 2008, 116: 243–260[15]McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11–13[16]Zhu Y-J(朱亚娟), Wang P(王鹏), Guo W-Z(郭旺珍), Zhang T-Z (张天真). Mapping QTLs for lint percentage and seed index using Gossypium barbadense chromosome segment introgression lines. Acta Agron Sin (作物学报), 2010, 36(8): 1318–1323 (in Chinese with English abstract)[17]Endrizzi J E. Linkage analysis of open bud and yellow petal (Y1) in cotton. Genome, 1991, 34: 461–63[18]Qian N, Zhang X W, Guo W Z, Zhang T Z. Fine mapping of open-bud duplicate genes in homoelogous chromosomes of tetraploid cotton. Euphytica, 2009, 165: 325–331[19]Liao C Y, Wu P, Hu B, Yi K K. Effects of genetic background and environment on QTLs and epistasis for rice (Oryza sativa L. ) panicle number. Theor Appl Genet, 2001, 103: 104–111[20]Eshed Y, Zamir D. Less-than-additive epistatic interactions of quantitative trait loci in tomato. Geneties, 1996, 143: 1807–1817[21]Lin Z-X(林忠旭), Feng C-H(冯常辉), Guo X-P(郭小平), Zhang X-L(张献龙). Genetic analysis of major QTLs and epistasis interaction for yield and fiber quality in upland cotton. Sci Agri Sin (中国农业科学), 2009, 42(9): 3036–3047 (in Chinese with English abstract)[22]Wang J K, Wan X Y, Li H H, Pfeiffer W H, Crouch J , Wan J M. Application of identified QTL-marker associations in rice quality improvement through a design-breeding approach. Theor Appl Genet, 2007, 115: 87–100[23]Wang Z-Q(王智权), Liu X(刘喜), Jiang L(江玲), Yang C(杨超), Liu S-J(刘世家), Chen L-M(陈亮明), Zhan H-Q(翟虎渠), Wan J-M(万建民). QTL detection for flag leaf morphological traits of rice in a population of chromosome segment substitution lines. J Nanjing Agri Univ (南京农业大学学报), 2010, 33(6): 1–6 (in Chinese with English abstract)[24]Ou-Yang L(欧阳恋). Identification, mapping and pyramiding of genes for grain quality based on SSSLs. MS thesis of South China Agricultural University, 2006 (in Chinese with English abstract)[25]Huang Y-F(黄益峰). Identification, pyramiding and epistasis analysis of the rice grain shape and grain weight QTL. MS thesis of South China Agricultural University, 2006 (in Chinese with English abstract) |
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