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作物学报 ›› 2014, Vol. 40 ›› Issue (06): 1020-1026.doi: 10.3724/SP.J.1006.2014.01020

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

一个化学诱变的小麦斑点叶突变体的生理和遗传分析

杜丽芬1,**,李明飞1,**,刘录祥2,王超杰1,刘洋1,许喜堂1,邹淑芳1,谢彦周1,*,王成社1,*   

  1. 1西北农林科技大学旱区作物逆境生物学国家重点实验室 / 农学院, 陕西杨凌 712100;  2中国农业科学院作物科学研究所, 北京100081
  • 收稿日期:2013-10-10 修回日期:2014-03-04 出版日期:2014-06-12 网络出版日期:2014-04-08
  • 通讯作者: 谢彦周, E-mail: xieyanzhou@gmail.com;王成社, E-mail: wangcs2008@126.com
  • 基金资助:

    本研究由国家自然科学基金项目(31101139),国家高技术研究发展计划(863计划)项目(2012AA101202), 陕西省统筹项目(2011KTZB02-01-01)和西北农林科技大学博士科研启动经费(2010BSJJ035)资助。

Physiological Characteristics and Genetic Analysis on a Spotted-Leaf Wheat Derived from Chemical Mutation

DU Li-Fen1,**,LI Ming-Fei1,**,LIU Lu-Xiang2,WANG Chao-Jie1,LIU Yang1,XU Xi-Tang1,ZOU Shu-Fang1,XIE Yan-Zhou1,*,WANG Cheng-She1,*   

  1. 1 State Key Laboratory of Crop Stress Biology in Arid Areas / College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-10-10 Revised:2014-03-04 Published:2014-06-12 Published online:2014-04-08
  • Contact: 谢彦周, E-mail: xieyanzhou@gmail.com;王成社, E-mail: wangcs2008@126.com

摘要:

通过EMS诱变普通小麦品系H261获得一个稳定遗传的斑点叶突变体LF2010。在自然条件下, 该突变体在三叶期叶片基部开始出现黄色斑点, 随后逐步扩散到全片叶、叶鞘、颖壳和麦芒。斑点部位不存在细胞死亡, 斑点性状的表达受光照和温度诱导, 突变体的色素含量、光合速率随着斑点的出现而显著下降。突变体的株高、有效穗数、单株产量、穗长、结实率和旗叶长等农艺性状显著下降, 但是千粒重和旗叶宽却与野生型无差异。将突变体与正常绿色品系杂交, 对其F1、F2和BC1代的遗传分析表明, LF2010的突变性状由1对隐性核基因控制。

关键词: 斑点叶突变, 光合色素含量, 光合特性, 农艺性状, 遗传分析

Abstract:

A light and temperature affected spotted-leaf mutant LF2010 (Triticum aestivum L.) was obtained from a mutagenic treatment with EMS on a wheat line H261. The bright yellow spot became evident once the third leaf will expanded, then covered later leaves and leaf sheaths of the whole plant including tissues of spike. The mutant plants differed from normal plants in terms of total chlorophyll content and net photosynthetic rate once symptom appeared on the leaves. The plant height, spike length, spike number per plant, grain weight per plant, grain number per spike, seed setting rate, and flag leaf length were lower in the mutant than in the wild type. However, the 1000-grain weight and flag leaf width of the mutant had no significant difference with those of the wild type. Genetic analysis based on F1, F2, and BC1 populations revealed that the yellow spot trait was controlled by a single recessive gene.

Key words: Spotted-leaf mutant, Photosynthetic pigments, Photosynthetic characteristics, Agronomic traits, Inheritance

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