作物学报 ›› 2014, Vol. 40 ›› Issue (09): 1585-1594.doi: 10.3724/SP.J.1006.2014.01585
霍冬英1,2,郑炜君1,李盼松1,2,徐兆师2,周永斌1,2,陈明2,马有志2,闵东红1,2,*,张小红1,2,*
HUO Dong-Ying1,2,ZHENG Wei-Jun1,LI Pan-Song1,2,XU Zhao-Shi2,ZHOU Yong-Bin1,2,CHEN Ming2,MA You-Zhi2,MIN Dong-Hong1,2,*,ZHANG Xiao-Hong1,2,*
摘要:
蛋白参与细胞周期调控、细胞凋亡及信号转导等多种生命活动对维持植物正常生长发育和介导非生阿物胁迫响应等过程发挥重要作用。谷子具有显著的耐旱耐瘠薄等特性本研究根据谷子转录组分析结果从个家族成员中鉴定出个在干旱胁迫下表达量上调的基因根据序列相似性将其分为类同一类基因具有相似的内含子外显子结构染色体定位分析发现这些基因分别分布在谷子的条染色体上其中第条染色体上含基因最多有个。结构域分析结果表明个蛋白均含保守的结构域而端含、、、、和等结构域。启动子元件分析表明谷子个基因均含逆境应答元件其中和元件的数量最多个说明这些基因对干旱应答反应可能主要受、转录因子调控。转录组分析结果表明基因对干旱胁迫的响应远远高于其他成员对干旱、高盐、、和都有响应亚细胞定位结果显示蛋白定位在细胞核中。本研究为进一步深入了解基因的功能提供了依据。F-box蛋白参与细胞周期调控、细胞凋亡及信号转导等多种生命活动, 对维持植物正常生长发育a和介导非生阿物胁迫响应等过程发挥重要作用。谷子具有显著的耐旱耐瘠薄等特性, 本研究根据谷子转录组分析结果, 从525个F-box家族成员中鉴定出19个在干旱胁迫下表达量上调的F-box基因; 根据序列相似性将其分为6类, 同一类基因具有相似的内含子-外显子结构; 染色体定位分析发现, 这些基因分别分布在谷子的8条染色体上, 其中, 第2条染色体上含F-box基因最多, 有6个。结构域分析结果表明, 19个F-box蛋白均含保守的F-box结构域, 而C端含FBD、WD40、FBA、ZnF、Kelch和LRR等结构域。启动子元件分析表明, 谷子19个F-box基因均含逆境应答元件, 其中, MYB和MYC元件的数量最多(9~78个), 说明这些基因对干旱应答反应可能主要受MYB、MYC转录因子调控。转录组分析结果表明, SiF-box18基因对干旱胁迫的响应远远高于其他F-box成员, 对干旱、高盐、ABA、SA和JA都有响应; 亚细胞定位结果显示, SiF-box18蛋白定位在细胞核中。本研究为进一步深入了解SiF-box18基因的功能提供了依据。
| [1]Verslues P E, Agarwal M, Katiyar A S, Zhu J, Zhu J K. Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J, 2006, 45: 523–539[2]Sadanandom A, Bailey M, Ewan R, Lee J, Nelis S. The ubiquitin-proteasome system: central modifier of plant signalling. New Phytol, 2012, 196: 13–28[3]Jackson P K, Eldridge A G, Freed E, Furstenthal L, Hsu J Y, Kaiser B K, Reimann J D. The lore of the RINGs: substrate recognition and catalysis by ubiquitin ligases. Trends Cell Biol, 2000, 10: 429–439[4]Cardozo T, Pagano M. The SCF ubiquitin ligase: insights into a molecular machine. Nat Rev Mol Cell Biol, 2004, 5: 739–751[5]Ho M S, Tsai P I, Chien C T. F-box proteins: the key to protein degradation. J Biomed Sci, 2006, 13: 181–191[6]Bai C, Richman R, Elledge S J. Human cyclin F. EMBO J, 1994, 13: 6087–6098[7]Levin J Z, Meyerowitz E M. UFO: an Arabidopsis gene involved in both floral meristem and floral organ development. Plant Cell, 1995, 7: 529–548[8]Hepworth S R, Klenz J E, Haughn G W. UFO in the Arabidopsis inflorescence apex is required for floral-meristem identity and bract suppression. Planta, 2006, 223:769–778[9]Sasaki K, Yamaguchi H, Aida R, Shikata M, Abe T, Ohtsubo N. Mutation in Torenia fournieri Lind: UFO homolog confers loss of TfLFY interaction and results in a petal to sepal transformation. Plant J, 2012, 71: 1002–1014[10]Wang Z, Chen J, Weng L, Li X, Cao X, Hu X, Luo D, Yang J. Multiple components are integrated to determine leaf complexity in Lotus japonicus. J Integr Plant Biol, 2013, 55: 419–433[11]Nelson D C, Lasswell J, Rogg L E, Cohen M A, Bartel B. FKF1, a clock-controlled gene that regulates the transition to flowering in Arabidopsis. Cell, 2000, 101: 331–340[12]Imaizumi T, Schultz T F, Harmon F G, Ho L A, Kay S A. FKF1F-box protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis. Science, 2005, 309: 293–297[13]Tomoyuki T, Yuuki N, Haruna T, Yasunobu O, Yuji M, Yumiko Y, Tomohiro K. LOV KELCH PROTEIN2 and ZEITLUPE repress Arabidopsis photoperiodic flowering under non-inductive conditions, dependent on FLAVIN-BINDING KELCH REPEAT F-BOX1. Plant J, 2011, 67: 608–621[14]Suetsugu N, Wada M. Evolution of three LOV blue light receptor families in green plants and photosynthetic stramenopiles: phototropin, ZTL/FKF1/LKP2 and aureochrome. Plant Cell Physiol, 2013, 54: 8–23[15]Lai Z, Ma W, Han B, Liang L, Zhang Y, Hong G, Xue Y. An F-box gene linked to the self-incompatibility locus of Antirrhinum is expressed specifically in pollen and tapetum. Plant Mol Biol, 2002, 50: 29–42[16]Ushijima K, Yamane H, Watari A, Kakehi E, Ikeda K, Hauck N R, Iezzoni A F, Tao R. The S haplotype-specific F-box protein gene, SFB, is defective in self-compatible haplotypes of Prunus avium and P. mume. Plant J, 2004, 39: 573–586[17]Hidenori S, Hiroyuki K, Mai M. Pollen-expressed F-box gene family and mechanism of S-RNase-based gametophytic self-incompatibility (GSI) in Rosaceae. Sex Plant Reprod, 2010, 23: 39–43[18]Wu J, Li M, Li T. Genetic Features of the Spontaneous Self-Compatible Mutant, ‘Jin Zhui’ (Pyrus bretschneideri Rehd.). PLoS One, 2013, 8, doi: 10.1371[19]Gray W M, Kepinski S, Rouse D, Leyser O, Estelle M. Auxin regulates SCF(TIR1)-dependent degradation of AUX/IAA proteins. Nature, 2001, 414: 271–276[20]Kepinski S, Leyser O. The Arabidopsis F-box protein TIR1 is an auxin receptor. Nature, 2005, 435: 446–451[21]Yu H, Moss B L, Jang S S, Prigge M, Klavins E, Nemhauser J L, Estelle M. Mutations in the TIR1 auxin receptor that increase affinity for auxin/indole-3-acetic acid proteins result in auxin hypersensitivity. Plant Physiol, 2013, 162: 295–303[22]Tromas A, Paque S, Stierlé V, Quettier A L, Muller P, Lechner E, Genschik P, Perrot-Rechenmann C. Auxin-Binding Protein 1 is a negative regulator of the SCF TIR1/AFB pathway. Nat Commun, 2013, 4: 2496[23]Guo H, Ecker J R. Plant responses to ethylene gas are mediated by SCF (EBF1/EBF2)-dependent proteolysis of EIN3 transcription factor. 2003, Cell, 115: 667–677[24]Iqbal N, Trivellini A, Masood A, Ferrante A, Khan N A. Current understanding on ethylene signaling in plants: The influence of nutrient availability. Plant Physiol Biochem, 2013, 73, 128–138[25]Itoh H, Matsuoka M, Steber C M. A role for the ubiquitin-26Sproteasome pathway in gibberellin signaling. Trends Plant Sci, 2003, 8: 492–497[26]Ariizumi T, Hauvermale A L, Nelson S K, Hanada A, Yamaguchi S, Steber C M. Lifting DELLA repression of Arabidopsis seed germination by nonproteolytic gibberellin signaling. Plant Physiol, 2013, 162: 2125–2139[27]Xie D X, Feys B F, James S, Nieto-Rostro M, Turner J G, COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility. Science, 1998, 280: 1091–1094[28]Devoto A, Ellis C, Magusin A, Chang H S, Chilcott C, Zhu T, Turner J G. Expression profiling reveals COI1 to be a key regulator of genes involved in wound-and methyl jasmonate-induced secondary metabolism, defence, and hormone interactions. Plant Mol Biol, 2005, 58: 497–513[29]Sasaki-Sekimoto Y, Jikumaru Y, Obayashi T, Saito H, Masuda S, Kamiya Y, Ohta H, Shirasu K. Basic helix-loop-helix transcription factors JASMONATE-ASSOCIATED MYC2-LIKE1 (JAM1), JAM2, and JAM3 are negative regulators of jasmonate responses in Arabidopsis. Plant Physiol, 2013, 163, 291–304[30]Zhang Y, Xu W, Li Z, Deng X W, Wu W, Xue Y. F-box protein DOR functions as a novel inhibitory factor for abscisic acid-induced stomatal closure under drought stress in Arabidopsis. Plant Physiol, 2008, 148:2121–2133[31]Bu Q, Lü T, Shen H, Luong P, Wang J, Wang Z, Huang Z, Xiao L, Engineer C, Kim T H, Schroeder J I, Huq E. Regulation of drought tolerance by the F-box protein MAX2 in Arabidopsis. Plant Physiol, 2013, doi: 10.1104[32]Ren J, Wen L, Gao X, Jin C, Xue Y, Yao X. DOG 1.0: illustrator of protein domain structures cell research. Cell Res, 2009, 19: 271–273[33]Yang S, Zhang X, Yue J X, Tian D, Chen J Q: Recent duplications dominate NBS-encoding gene expansion in two woody species. Mol Genet Genom, 2008, 280: 187–198[34]Liu P, Xu Z S, Pan-Pan L, Hu D, Chen M, Li L C, Ma Y Z. A wheat plasma membrane-localized PI4K gene possessing threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis. J Exp Bot, 2013, 64: 2915–2927[35]Jin J, Cardozo T, Lovering R C, Elledge S J, Pagano M, Harper J W. Systematic analysis and nomenclature of mammalian F-box proteins. Genes Dev, 2004, 18: 2573–2580[36]Gagne J M, Downes B P, Shiu S H, Durski A M, Vierstra R D. The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis. Proc Natl Acad Sci USA, 2002, 99: 11519–11524[37]Kuroda H, Takahashi N, Shimada H, Seki M, Shinozaki K, Matsui M. Classification and expression analysis of Arabidopsis F-box-containing protein genes. Plant Cell Physiol, 2002, 43: 1073–1085[38]Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi A K, Khurana J P. F-box proteins in rice. Genomewide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress. Plant Physiol, 2007, 143: 1467–1483[39]Jia F, Wu B, Li H, Huang J, Zheng C. Genome-wide identification and characterization of F-box family in maize. Mol Genet Genomics, 2013, 288: 559–577[40]Andrade M A, Perez-Iratxeta C, Ponting C P. Protein repeats: structures, functions, and evolution. J Struct Biol, 2001, 134: 117–131[41]Smith T F, Gaitatzes C, Saxena K, Neer E J. The WD repeat: a common architecture for diverse functions. Trends Biochem Sci, 1999, 24: 181–185[42]Craig K L, Tyers M. The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. Prog Biophys Mol Biol, 1999, 72: 299–328[43]Koops P, Pelser S, Ignatz M, Klose C, Marrocco-Selden K, Kretsch T. EDL3 is an F-box protein involved in the regulation of abscisic acid signalling in Arabidopsis thaliana. J Exp Bot, 2011, 62: 5547–5560 |
| [1] | 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812. |
| [2] | 张晴, 杨昱, 郭茜, 岳霈尧, 殷丛丛, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 大豆GmARA6a的克隆及响应盐胁迫的功能分析[J]. 作物学报, 2026, 52(2): 480-493. |
| [3] | 余开航, 周洪斌, 罗亮扎, 王玫郦, 姜瑞梅, 董陈文华, 李仕金, 毛孝强, 陈升位. 大麦亮氨酸富集重复型类受体激酶基因HvLRR-RLK-510的克隆和表达分析[J]. 作物学报, 2026, 52(2): 421-432. |
| [4] | 王若楠, 张颖星, 于筱菡, 刘少雄, 王跃, 薛亚鹏, 辛旭霞, 张莉, 刘敏轩. 基于近红外快速检测技术的谷子淀粉多样性分析及模型构建[J]. 作物学报, 2025, 51(7): 1757-1768. |
| [5] | 沈傲, 刘敏, 倪迪安, 刘炜. 谷子m6A甲基转移酶基因SiMTA1的启动子序列特征和基因表达模式分析[J]. 作物学报, 2025, 51(7): 1969-1978. |
| [6] | 梁红凯, 赵苏蒙, 陆琼, 周鹏, 智慧, 刁现民, 贺强. 谷子微核心种质的构建[J]. 作物学报, 2025, 51(6): 1435-1444. |
| [7] | 潘炬忠, 韦萍, 朱德平, 邵胜雪, 陈珊珊, 韦雅倩, 高维维. 水稻转录因子OsERF104的克隆和功能研究[J]. 作物学报, 2025, 51(4): 900-913. |
| [8] | 郭冰, 秦家范, 李娜, 宋梦瑶, 王黎明, 李君霞, 马小倩. 谷子SHMT基因家族全基因组鉴定与表达分析[J]. 作物学报, 2025, 51(3): 586-5897. |
| [9] | 朱灿灿, 李君霞, 景雅, 付森杰, 秦娜, 王春义, 代书桃, 魏昕, 张程炀. 不同耐荫性谷子对遮阴的生理响应及转录组分析[J]. 作物学报, 2025, 51(12): 3211-3223. |
| [10] | 王玉娇, 王永乐, 添长久, 郁春旺, 吕佳斌, 朱加保. 薏苡VQ4基因的克隆及耐盐性初步分析[J]. 作物学报, 2025, 51(12): 3198-3210. |
| [11] | 王媛, 许佳茵, 董二伟, 王劲松, 刘秋霞, 黄晓磊, 焦晓燕. 有机肥替代化肥氮对谷子氮素累积、产量及品质的影响[J]. 作物学报, 2025, 51(1): 149-160. |
| [12] | 孟凡花, 刘敏, 沈傲, 刘炜. 脂质转移蛋白SiLTP1基因参与谷子耐盐响应初探[J]. 作物学报, 2025, 51(1): 58-67. |
| [13] | 闫锋, 董扬, 李清泉, 赵富阳, 侯晓敏, 刘洋, 李青超, 赵蕾, 范国权, 刘凯. 谷子育成品种萌芽期耐冷性综合评价[J]. 作物学报, 2024, 50(9): 2207-2218. |
| [14] | 高维东, 胡城祯, 张龙, 张艳艳, 张沛沛, 杨德龙, 陈涛. 小麦泛素结合酶TaUBC16基因的克隆与功能分析[J]. 作物学报, 2024, 50(8): 1971-1988. |
| [15] | 秦娜, 叶珍言, 朱灿灿, 付森杰, 代书桃, 宋迎辉, 景雅, 王春义, 李君霞. 谷子籽粒类黄酮含量和粒色的QTL定位[J]. 作物学报, 2024, 50(7): 1719-1727. |
|
||