作物学报 ›› 2014, Vol. 40 ›› Issue (09): 1595-1603.doi: 10.3724/SP.J.1006.2014.01595
邹筱,韩粉霞,陈明阳,孙君明,南金平,闫淑荣,杨华
ZOU Xiao,HAN Fen-Xia,CHEN Ming-Yang,SUN Jun-Ming,NAN Jin-Ping,YAN Shu-Rong,YANG Hua
摘要:
以中黄13×中黄20的100个BC2F2家系为作图群体,构建了一张包含131个SSR分子标记的遗传连锁图谱,图谱总长为2157.3 cM,平均遗传距离为16.5 cM,涵盖了大豆的20个连锁群。利用气相色谱技术测定BC2F2、BC2F3和BC2F4回交群体的脂肪酸主要组分含量,采用IciMapping 3.3完备区间作图法定位QTL,共检测到5种脂肪酸组分相关的QTL 26个,与棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸相关的QTL分别为5、5、7、5和4个;3个区间在不同年份被检测到与同一脂肪酸组分相关,sat_294~satt228连续3年被检测到与棕榈酸含量相关,sat_253~satt323和sat_292~satt397连续2年被检测到与油酸含量相关;4个区间被检测到与2种脂肪酸组分相关,其中sat_294~satt228与棕榈酸、油酸相关,satt308~sat_422与硬脂酸、亚油酸相关,sat_292~satt397与油酸、亚油酸相关,satt374~satt269与油酸、亚麻酸相关。
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