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作物学报 ›› 2014, Vol. 40 ›› Issue (09): 1585-1594.doi: 10.3724/SP.J.1006.2014.01585

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

谷子F-box家族基因的鉴定、分类及干旱响应

霍冬英1,2,郑炜君1,李盼松1,2,徐兆师2,周永斌1,2,陈明2,马有志2,闵东红1,2,*,张小红1,2,*   

  1. 1旱区作物逆境生物学国家重点实验室 / 西北农林科技大学, 陕西杨凌 712100; 2中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 / 农业部麦类生物学与遗传育种重点实验室, 北京 100081
  • 收稿日期:2013-12-01 修回日期:2014-06-16 出版日期:2014-09-12 网络出版日期:2014-07-09
  • 通讯作者: 张小红, E-mail: zhxh2493@126.com; 闵东红, E-mail: mdh2493@126.com
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2014ZX08002-03B, 2011ZX08002002)资助。

Identification, Classification, and Drought Response of F-box Gene Family in Foxtail Millet

HUO Dong-Ying1,2,ZHENG Wei-Jun1,LI Pan-Song1,2,XU Zhao-Shi2,ZHOU Yong-Bin1,2,CHEN Ming2,MA You-Zhi2,MIN Dong-Hong1,2,*,ZHANG Xiao-Hong1,2,*   

  1. 1 State Key Laboratory of Crop Stress Biology for Arid Areas / Northwest Agricultural and Forestry University, Yangling 712100, China; 2 Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Beijing 100081, China?
  • Received:2013-12-01 Revised:2014-06-16 Published:2014-09-12 Published online:2014-07-09
  • Contact: 张小红, E-mail: zhxh2493@126.com; 闵东红, E-mail: mdh2493@126.com

摘要:

蛋白参与细胞周期调控、细胞凋亡及信号转导等多种生命活动对维持植物正常生长发育和介导非生阿物胁迫响应等过程发挥重要作用。谷子具有显著的耐旱耐瘠薄等特性本研究根据谷子转录组分析结果家族成员中鉴定出个在干旱胁迫下表达量上调的基因根据序列相似性将其分为同一类基因具有相似的内含子外显子结构染色体定位分析发现这些基因分别分布在谷子的条染色体上其中条染色体上含基因最多个。结构域分析结果表明蛋白均含保守的结构域端含等结构域。启动子元件分析表明谷子基因均含逆境应答元件其中元件的数量最多说明这些基因对干旱应答反应可能主要受转录因子调控。转录组分析结果表明基因对干旱胁迫的响应远远高于其他成员对干旱、高盐、都有响应亚细胞定位结果显示蛋白定位在细胞核中。本研究为进一步深入了解基因的功能提供了依据。F-box蛋白参与细胞周期调控、细胞凋亡及信号转导等多种生命活动, 对维持植物正常生长发育a和介导非生阿物胁迫响应等过程发挥重要作用。谷子具有显著的耐旱耐瘠薄等特性, 本研究根据谷子转录组分析结果, 525F-box家族成员中鉴定出19个在干旱胁迫下表达量上调的F-box基因; 根据序列相似性将其分为6, 同一类基因具有相似的内含子-外显子结构; 染色体定位分析发现, 这些基因分别分布在谷子的8条染色体上, 其中, 2条染色体上含F-box基因最多, 6。结构域分析结果表明, 19F-box蛋白均含保守的F-box结构域, C端含FBDWD40FBAZnFKelchLRR等结构域。启动子元件分析表明, 谷子19F-box基因均含逆境应答元件, 其中, MYBMYC元件的数量最多(9~78), 说明这些基因对干旱应答反应可能主要受MYBMYC转录因子调控。转录组分析结果表明, SiF-box18基因对干旱胁迫的响应远远高于其他F-box成员, 对干旱、高盐、ABASAJA都有响应; 亚细胞定位结果显示, SiF-box18蛋白定位在细胞核中。本研究为进一步深入了解SiF-box18基因的功能提供了依据。

关键词: 谷子, F-box蛋白, 亚细胞定位, 干旱响应, 诱导机制

Abstract:

F-box proteins play critical roles in plant growth and development and respond to abiotic stresses, involving in cell-cycle regulation, cell apoptosis and signal transduction. Foxtail millet has a character with drought-tolerance. We identified 19 up-regulated F-box genes from 525 F-box families under stress in foxtail millet based on the transcriptome sequencing and divided them into six subgroups, and the genes in the same subgroup had the same intron-exon structure. These genes were unevenly distributed on eight foxtail millet chromosomes, and there were six genes on chromosome 2, that gene number was more than that contained on other chromosomes. The analysis of conserved domain of 19 genes showed that all the protein sequences had a conserved F-box motif, and several other domain also existed in C-terminal including FBD, WD40, FBA, ZnF, and Kelch LRR. Putative cis-acting elements showed that the number of MYB and MYC was the most, which ranged from 9 to 78. Transcriptome sequencing showed that SiF-box18 was more hypersensitive to the drought stress than other F-box members and response to drought, salt, ABA, GA, SA and JA based on the real time PCR. The transient expression in Arabidopsis protoplasts displayed that SiF-box18 protein was localized in nuclear section. This study provided experimental data for further studying the functions of the SiF-box18 gene.

Key words: Foxtail millet, F-box protein, Subcellular localization, Drought response, Induction mechanism

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