作物学报 ›› 2014, Vol. 40 ›› Issue (09): 1702-1709.doi: 10.3724/SP.J.1006.2014.01702
• 研究简报 • 上一篇
曹红利,岳川,周艳华,王璐,郝心愿,杨亚军*,王新超*
CAO Hong-Li,YUE Chuan,ZHOU Yan-Hua,WANG Lu,HAO Xin-Yuan,YANG Ya-Jun*,WANG Xin-Chao*
摘要:
碱性亮氨酸拉链蛋白(bZIP)作为真核生物中分布最广、最保守的一类转录因子,参与多种生物学过程,尤其在植物抵御各种逆境胁迫中有重要作用。采用RACE和RT-PCR技术克隆到茶树bZIP转录因子基因全长cDNA序列,命名为CsbZIP1(GenBank登录号为JX050148.1)。该基因cDNA全长1515 bp,包含813 bp的完整开放阅读框(ORF),编码270个氨基酸,预测分子量29.484 kD;含有bZIP家族典型的BRLZ结构域碱性结构域和亮氨酸拉链,属于B-zip1家族;系统发育树分析显示CsbZIP1属于bZIP转录因子F亚家族;亚细胞定位结果表明CsbZIP1主要定位于细胞核;qRT-PCR分析表明,4℃低温和NaCl盐胁迫处理均能诱导CsbZIP1的表达,表达量变化趋势都是随着胁迫时间先逐渐升高,到24 h时降低,ABA胁迫处理24 h抑制CsbZIP1的表达。推测CsbZIP1与茶树低温、盐等逆境胁迫密切相关。
| [1]Xu Z S, Chen M, Li L C, Ma Y Z. Functions and application of the AP2/ERF transcription factor family in crop improvement. J Integr Plant Biol, 2011, 53: 570–585[2]Landschulz W, Johnson P, McKnight S. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science, 1988, 240: 1759–1764[3]Kim S, Kang J Y, Cho D I, Park J H, Kim S Y. ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance. Plant J, 2004, 40: 75–87[4]Jakoby M, Weisshaar B, Droge-Laser W, Vicente-Carbajosa J, Tiedemann J, Kroj T, Parcy F. bZIP transcription factors in Arabidopsis. Trends Plant Sci, 2002, 7: 106–111[5]Nijhawan A, Jain M, Tyagi A K, Khurana J P. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice. Plant Physiol, 2008, 146: 333–350[6]Gao S Q, Chen M, Xu Z S, Zhao C P, Li L, Xu H J, Tang Y M, Zhao X, Ma Y Z. The soybean GmbZIP1 transcription factor enhances multiple abiotic stress tolerances in transgenic plants. Plant Mol Biol, 2011, 75: 537–553[7]Rodriguez-Uribe L, O'Connell M A. A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris). J Exp Bot, 2006, 57: 1391–1398[8]Liao Y, Zou H F, Wei W, Hao Y J, Tian A G, Huang J, Liu Y F, Zhang J S, Chen S Y. Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta, 2008, 228: 225–240[9]Liu C, Wu Y, Wang X. bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice. Planta, 2012, 235: 1157–1169[10]Kang J Y. Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell Online, 2002, 14: 343–357[11]Ying S, Zhang D F, Fu J, Shi Y S, Song Y C, Wang T Y, Li Y. Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis. Planta, 2012, 235: 253–266[12]Hsieh T H, Li C W, Su R C, Cheng C P, Sanjaya, Tsai Y C, Chan M T. A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response. Planta, 2010, 231: 1459–1473[13]Wang J, Zhou J, Zhang B, Vanitha J, Ramachandran S, Jiang S Y. Genome-wide expansion and expression divergence of the basic leucine zipper transcription factors in higher plants with an emphasis on sorghum. J Integr Plant Biol, 2011, 53: 212–231[14]Lee S C, Choi H W, Hwang I S, Choi du S, Hwang B K. Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIP1, in enhanced resistance to pathogen infection and environmental stresses. Planta, 2006, 224: 1209–1225[15]周精华, 揭雨成, 邢虎成, 钟英丽, 余伟林. 苎麻BnbZIP1转录因子基因的克隆与表达特征分析. 中国农业科学, 2013, 46: 1314–1322Zhou J H, Jie Y C, Xing H C, Zhong L Y, Yu W L. Cloning and characterization of the BnbZIP1 transcription factor gene from ramie (Boehmeria nivea L.). Sci Agric Sin, 2013, 46: 1314–1322 (in Chinese with English abstract)[16]Wang Y, Gao C, Liang Y, Wang C, Yang C, Liu G. A novel bZIP gene from Tamarix hispida mediates physiological responses to salt stress in tobacco plants. J Plant Physiol, 2010, 167: 222–230[17]Chen H, Chen W, Zhou J, He H, Chen L, Chen H, Deng X W. Basic leucine zipper transcription factor OsbZIP16 positively regulates drought resistance in rice. Plant Sci, 2012, 193–194: 8–17[18]Wang X C, Zhao Q Y, Ma C L, Zhang Z H, Cao H L, Kong Y M, Yue C, Hao X Y, Chen L, Ma J Q, Jin J Q, Li X, Yang Y J. Global transcriptome profiles of Camellia sinensis during cold acclimation. BMC Genomics, 2013, 14: 415[19]Paul A, Lal L, Ahuja P S, Kumar S. Alpha-tubulin (CsTUA) up-regulated during winter dormancy is a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze]. Mol Biol Rep, 2012, 39: 3485–3490[20]Wang Y, Jiang C J, Li Y Y, Wei C L, Deng W W. 2012. CsICE1 and CsCBF1: two transcription factors involved in cold responses in Camellia sinensis. Plant Cell Rep, 2012, 31: 27–34[21]郝姗. 茶树不同逆境条件下QRT-PCR适宜内参基因的筛选. 南京农业大学硕士学位论文, 江苏南京, 2012. pp 38–65Hao S. Selection of Appropriate Reference Genes for Expression Studies in Camellia sinensis by Real-time Polymerase Chain Reaction. MS Thesis of Nanjing Agricultural University, Nanjing, China, 2012. pp 38–65 (in Chinese with English abstract)[22]曹红利, 岳川, 郝心愿, 王新超, 杨亚军. 茶树胆碱单加氧酶CsCMO的克隆及甜菜碱合成关键基因的表达分析. 中国农业科学, 2013, 46: 3087–3096Cao H L, Yue C, Hao X Y, Wang X C, Yang Y J. Cloning of choline monooxygenase (CMO) gene and expression analysis of the key glycine betaine biosynthesis-related genes in tea plant (Camellia sinensis). Sci Agric Sin, 2013, 46: 3087–3096 (in Chinese with English abstract)[23]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 2001, 25: 402–408[24]Kumimoto R W, Siriwardana C L, Gayler K K, Risinger J R, Siefers N, Holt B F. Nuclear factor Y transcription factors have both opposing and additive roles in ABA-mediated seed germination. PLoS One, 2013, 8: e59481[25]Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci USA, 2000, 97: 11632–11637[26]Yamaguchi-Shinozaki K, Shinozaki K. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol, 2006, 57: 781–803[27]Wang B, Zheng J, Liu Y, Wang J, Wang G. Cloning and characterization of the stress-induced bZIP gene ZmbZIP60 from maize. Mol Biol Rep, 2012, 39: 6319–6327[28]Zou M, Guan Y, Ren H, Zhang F, Chen F. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance. Plant Mol Biol, 2008, 66: 675–683[29]Orellana S, Yanez M, Espinoza A, Verdugo I, Gonzalez E, Ruiz-Lara S, Casaretto J A. The transcription factor SlAREB1 confers drought, salt stress tolerance and regulates biotic and abiotic stress-related genes in tomato. Plant Cell Environ, 2010, 33: 2191–2208[30]Schlogl P S, Nogueira F T, Drummond R, Felix J M, De Rosa V E, Jr.Vicentini R, Leite A, Ulian E C, Menossi M. Identification of new ABA- and MEJA-activated sugarcane bZIP genes by data mining in the SUCEST database. Plant Cell Rep, 2008, 27: 335–345[31]Cheng C, Yun K Y, Ressom H W, Mohanty B, Bajic V B, Jia Y, Yun S J, de los Reyes B G. An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice. BMC Genomics, 2007, 8: 175 |
| [1] | 徐苗苗, 邸太妹, 王洁, 吴叶蝶, 刘恩贝, 王玉春, 王新超, 王璐. 外源槲皮素增强茶树抗寒性的分子机制[J]. 作物学报, 2026, 52(5): 1418-1429. |
| [2] | 杨影, 郝豫皖, 张学宁, 方佳璐, 马月华, 杨伟龙, 孙文清, 王新超, 王玉春, 黄建燕. 乙烯响应因子CsERF9调控茶树炭疽病抗性的分子机制研究[J]. 作物学报, 2026, 52(4): 1103-1115. |
| [3] | 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812. |
| [4] | 张晴, 杨昱, 郭茜, 岳霈尧, 殷丛丛, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 大豆GmARA6a的克隆及响应盐胁迫的功能分析[J]. 作物学报, 2026, 52(2): 480-493. |
| [5] | 余开航, 周洪斌, 罗亮扎, 王玫郦, 姜瑞梅, 董陈文华, 李仕金, 毛孝强, 陈升位. 大麦亮氨酸富集重复型类受体激酶基因HvLRR-RLK-510的克隆和表达分析[J]. 作物学报, 2026, 52(2): 421-432. |
| [6] | 张力岚, 杨军, 王让剑. 基于WGCNA发掘茶树糖苷类香气前体含量性状相关的候选基因[J]. 作物学报, 2026, 52(2): 494-513. |
| [7] | 梅飘, 刘丁丁, 叶圆圆, 张晨禹, 丁诗琦, 李亚奇, 王培鑫, 梅菊芬, 马春雷. 基于茶树液相功能芯片的白化茶树资源遗传多样性分析[J]. 作物学报, 2025, 51(9): 2358-2370. |
| [8] | 潘炬忠, 韦萍, 朱德平, 邵胜雪, 陈珊珊, 韦雅倩, 高维维. 水稻转录因子OsERF104的克隆和功能研究[J]. 作物学报, 2025, 51(4): 900-913. |
| [9] | 王玉娇, 王永乐, 添长久, 郁春旺, 吕佳斌, 朱加保. 薏苡VQ4基因的克隆及耐盐性初步分析[J]. 作物学报, 2025, 51(12): 3198-3210. |
| [10] | 高维东, 胡城祯, 张龙, 张艳艳, 张沛沛, 杨德龙, 陈涛. 小麦泛素结合酶TaUBC16基因的克隆与功能分析[J]. 作物学报, 2024, 50(8): 1971-1988. |
| [11] | 张力岚, 杨军, 王让剑. 茶树橙花叔醇和芳樟醇樱草糖苷含量全基因组关联分析及候选基因预测[J]. 作物学报, 2024, 50(4): 871-886. |
| [12] | 张宝华, 刘佳静, 田晓, 田旭钊, 董阔, 武郁洁, 肖凯, 李小娟. 小麦TaSPX1基因的克隆、表达及耐低氮逆境的功能研究[J]. 作物学报, 2024, 50(3): 576-589. |
| [13] | 代洪苇, 刘洁强, 张丽, 童华荣, 袁连玉. 茶树CsMCC1和CsMCC2基因的克隆及表达特征性分析[J]. 作物学报, 2024, 50(3): 656-668. |
| [14] | 黄钰杰, 张啸天, 陈会丽, 王宏伟, 丁双成. 玉米ZmC2s基因家族鉴定及ZmC2-15耐热功能分析[J]. 作物学报, 2023, 49(9): 2331-2343. |
| [15] | 王让剑, 杨军, 张力岚, 高香凤. 茶树新梢中香叶醇樱草糖苷含量的全基因组关联分析[J]. 作物学报, 2023, 49(7): 1843-1859. |
|
||