作物学报 ›› 2014, Vol. 40 ›› Issue (11): 1946-1955.doi: 10.3724/SP.J.1006.2014.01946
赵晨晨1,黄福灯2,龚盼1,杨茜1,程方民1,潘刚1,*
ZHAO Chen-Chen1,HUANG Fu-Deng2,GONG Pan1,YANG Xi1,CHENG Fang-Min1,PAN Gang1,*
摘要:
叶片早衰直接影响作物的产量与品质, 因此, 研究叶片早衰的分子与生理机制对于作物遗传改良具有重要的意义。本研究利用60Co辐射诱变水稻品种93-11获得突变体osled, 其从分蘖期叶片就开始早衰, 最先表现为叶尖和叶边缘变褐, 并伴有红褐色斑点。在苗期经模拟干旱胁迫处理后, 突变体不仅早衰, 而且植株变矮以及根系变短。生理分析表明, 野生型剑叶、倒二叶和倒三叶的丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及过氧化物酶(POD)活性基本不变, 但突变体则显著升高且倒二叶和倒三叶极显著高于野生型; 突变体和野生型三片叶的可溶性蛋白含量、过氧化氢酶(CAT)活性及叶绿素总含量均依次下降, 但突变体倒二叶和倒三叶的含量或活性均显著低于野生型。叶片经台盼蓝、二氨基联苯胺(DAB)及四唑硝基蓝(NBT)等细胞组织化学染色及透射电镜分析表明, osled叶片细胞膜系统已破坏, H2O2和O2?积累, 叶绿体已开始解体。遗传分析表明, osled受一隐性基因控制, 借助图位克隆技术将该基因定位于第3染色体长臂的RM15528与RM15553两个标记之间, 遗传距离均为0.7 cM, 该结果为进一步克隆OsLED基因并研究其功能奠定了基础。
| [1]Lim P O, Kim H J, Nam H G. Leaf senescence. Annu Rev Plant Biol, 2007, 58: 115–136[2]杨建昌, 朱庆森, 王志琴, 郎有忠. 亚种间杂交稻光合特性及物质积累与运转的研究. 作物学报, 1997, 23: 82–88Yang J C, Zhu Q S, Wang Z Q, Lang Y Z. Photosynthetic characteristics, dry-matter accumulation and its translocation in intersubspecific hybrid rice. Acta Agron Sin, 1997, 23: 82–88 (in Chinese with English abstract)[3]梁建生, 曹显祖. 杂交水稻叶片的若干生理指标与根系伤流强度关系. 江苏农学院学报, 1993, 14: 25–30 Liang J S, Cao X Z. Studies on the relationship between several physiological characteristics of leaf and bleeding rate of roots in hybrid rice. Jiangsu Agric Coll, 1993, 14: 25–30 (in Chinese with English abstract)[4]Liu L, Zhou Y, Zhou G, Ye R, Zhao L, Li X, Lin Y. Identification of early senescence-associated genes in rice flag leaves. Plant Mol Biol, 2008, 67: 37–55[5]Kong Z, Li M, Yang W, Xu W, Xue Y. A novel nuclear-localized CCCH-type zinc finger protein, OsDOS, is involved in delaying leaf senescence in rice. Plant Physiol, 2006, 141: 1376–1388[6]Jan A, Maruyama K, Todaka D, Kidokoro S, Abo M, Yoshimura E, Shinozaki K, Nakashima K, Yamaguchi-Shinozaki K. OsTZF1, a CCCH-tandem zinc finger protein, confers delayed senescence and stress tolerance in rice by regulating stress-related genes. Plant Physiol, 2013, 161: 1202–1216[7]Hudson D, Guevara D R, Hand A J, Xu Z, Hao L, Chen X, Zhu T, Bi Y M, Rothstein S J. Rice cytokinin GATA transcription Factor1 regulates chloroplast development and plant architecture. Plant Physiol, 2013, 162: 132–144[8]Zhou Y, Huang W, Liu L, Chen T, Zhou F, Lin Y. Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence. BMC Plant Biol, 2013, 13: 132[9]Fukao T, Yeung E, Bailey-Serres J. The submergence tolerance gene SUB1A delays leaf senescence under prolonged darkness through hormonal regulation in rice. Plant Physiol, 2012, 160: 1795–1807[10]Luan W, Shen A, Jin Z, Song S, Li Z, Sha A. Knockdown of OsHox33, a member of the class III homeodomain-leucine zipper gene family, accelerates leaf senescence in rice. Sci China Life Sci, 2013, 56: 1113–1123[11]Singh S, Giri M K, Singh P K, Siddiqui A, Nandi A K. Down-regulation of OsSAG12-1 results in enhanced senescence and pathogen-induced cell death in transgenic rice plants. J Biosci, 2013, 38: 583–592[12]Gao Q, Yang Z, Zhou Y, Yin Z, Qiu J, Liang G, Xu C. Characterization of an Abc1 kinase family gene OsABC1-2 conferring enhanced tolerance to dark-induced stress in rice. Gene, 2012, 498: 155-163[13]Chen L J, Wuriyanghan H, Zhang Y Q, Duan K X, Chen H W, Li Q T, Lu X, He S J, Ma B, Zhang W K, Lin Q, Chen S Y, Zhang J S, An S-domain receptor-like kinase, OsSIK2, confers abiotic stress tolerance and delays dark-induced leaf senescence in rice. Plant Physiol, 2013, 163: 1752–1765[14]Lee R H, Lin M C, Chen S C. A novel alkaline alpha-galactosidase gene is involved in rice leaf senescence. Plant Mol Biol, 2004, 55: 281-295[15]Jiang H, Li M, Liang N, Yan H, Wei Y, Xu X, Liu J, Xu Z, Chen F, Wu G. Molecular cloning and function analysis of the stay green gene in rice. Plant J, 2007, 52: 197–209 [16]Park S Y, Yu J W, Park J S, Li J, Yoo S C, Lee N Y, Lee S K, Jeong S W, Seo H S, Koh H J, Jeon J S, Park Y I, Paek N C. The senescence-induced stay green protein regulates chlorophyll degradation. Plant Cell, 2007, 19: 1649–1664[17]Rong H, Tang Y, Zhang H, Wu P, Chen Y, Li M, Wu G, Jiang H. The Stay-Green Rice like (SGRL) gene regulates chlorophyll degradation in rice. Plant Physiol, 2013, 170: 1367–1373[18]Lee R H, Hsu J H, Huang H J, Lo S F, Chen S C. Alkaline alpha-galactosidase degrades thylakoid membranes in the chloroplast during leaf senescence in rice. New Phytol, 2009, 184: 596–606[19]Jiao B B, Wang J J, Zhu X D, Zeng L J, Li Q, He Z H. A novel protein RLS1 with NB-ARM domains is involved in chloroplast degradation during leaf senescence in rice. Mol Plant, 2012, 5: 205–217[20]Sato Y, Morita R, Katsuma S, Nishimura M, Tanaka A, Kusaba M. Two short-chain dehydrogenase/reductases, NON-YELLOW COLORING 1 and NYC1-LIKE, are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice. Plant J, 2009, 57: 120–131[21]Kusaba M, Ito H, Morita R, Lida S, Sato Y, Fujimoto M, Kawasaki S, Tanaka R, Hirochika H, Nishimura M, Tanaka A. Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence. Plant Cell, 2007, 19: 1362–1375[22]Morita R, SatoY, Masuda Y, Nishimura M, Kusaba M. Defect in NON YELLOW COLORING 3, an α/β hydrolase-fold family protein, causes a stay green phenotype during leaf senescence in rice. Plant J, 2009, 59: 940–952[23]Yamatani H, Sato Y, Masuda Y, Kato Y, Morita R, Fukunaga K, Nagamura Y, Nishimura M, Sakamoto W, Tanaka A, Kusaba M. NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll- protein complexes during leaf senescence. Plant J, 2013, 74: 652–662[24]Tang Y, Li M, Chen Y, Wu P, Wu G, Jiang H. Knockdown of OsPAO and OsRCCR1 cause different plant death phenotypes in rice. Plant Physiol, 2011, 168: 1952–1959[25]孙波, 周勇, 林拥军. 一个水稻衰老上调表达基因的初步生物学功能分析. 作物学报, 2012, 38: 1988–1996Sun B, Zhou Y, Lin Y J. Preliminary functional analysis of a rice leaf senescence up-regulated gene. Acta Agron Sin, 2012, 38: 1988–1996 (in Chinese with English abstract)[26]Mohammad I A, Ruey-Hua L, Shu-Chen G C. A novel senescence-associated gene encoding γ-aminobutyric acid (GABA): pyruvate transaminase is upregulated during rice leaf senescence. Physiol Plant, 2004, 123: 1–8[27]Qiao Y, Jiang W, Lee J, Park B, Choi M S, Piao R, Woo M O, Roh J H, Han L, Paek N C, Seo H S, Koh H J. SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit micro 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). New Phytol, 2010, 185: 258–274[28]Zhou Q, Yu Q, Wang Z, Pan Y, Lv W, Zhu L, Chen R, He G. Knockdown of GDCH gene reveals reactive oxygen species-induced leaf senescence in rice. Plant Cell Environ, 2013, 36: 1476–1489[29]Pitakrattananukool S, Kawakatsu T, Anuntalabhochai S, Takaiwa F. Overexpression of OsRab7B3, a small GTP-binding protein gene, enhances leaf senescence in transgenic rice. Biosci Biotechnol Biochem, 2012, 76: 1296–1302[30]Undan J R, Tamiru M, Abe A, Yoshida K, Kosugi S, Takagi H, Yoshida K, Kanzaki H, Saitoh H, Fekih R, Sharma S, Undan J, Yano M, Terauchi R. Mutation in OsLMS, a gene encoding a protein with two double-stranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (Oryza sativa L.). Genes Genet Syst, 2012, 87: 169–179[31]李燕群, 高家旭, 肖云华, 李秀兰, 蒲翔, 孙昌辉, 王平荣, 邓晓健. 水稻ygl80黄绿叶突变体的遗传分析与目标基因精细定位. 作物学报, 2014, 40: 644–649Li Y Q, Gao J X, Xiao Y H, Li X L, Pu X, Sun C H, Wang P R, Deng X J. Genetic analysis and gene fine mapping of yellow-green leaf mutant ygl80 in rice. Acta Agron Sin, 2014, 40: 644–649 (in Chinese with English abstract)[32]Yoshida S, Forno D A, Cock J H, Gomez K A. Laboratory Manual for Physiological Studies of Rice. Philippines: IRRI, 1976[33]陈建勋, 王晓峰. 植物生理学实验指导. 广东: 华南理工大学出版社, 2002. pp 35-128 Chen J X, Wang X F. Experiments Instructions of Plant Physiology. Guangdong: Huanan Technology university Publishers, 2002. pp 35–128 (in Chinese)[34]Yin Z, Chen J, Zeng L, Goh M, Leung H, Khush G S, Wang G L. Characterizing rice lesion mimic mutants and identifying a mutant with broad-spectrum resistance to rice blast and bacterial blight. Mol Plant-Microbe Interact, 2000, 13: 869–876[35]Kariola T, Brader G, Li J, Palva E T. Chlorophyllase 1, a damage control enzyme, affects the balance between defense pathways in plants. Plant Cell, 2005, 17: 282–294[36]Mahalingam R, Jambunathan N, Gunjan S K, Faustin E, Weng H, Ayoubi P. Analysis of oxidative signalling induced by ozone in Arabidopsis thaliana. Plant Cell Environ, 2006, 29: 1357–1371[37]Shen Y , Jiang H, Jin J, Zhang Z, Xi B, He Y, Wang G, Wang C, Qian L, Li X, Yu Q, Liu H, Chen D, Gao J, Huang H, Shi T, Yang Z. Development of genome-wide DNA polymorphism database for map-based cloning of rice genes. Plant Physiol, 2004, 135: 1198–1205[38]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSR) in rice (Oryza sativa L.). Mol Gen Genet, 1996, 252: 597–607[39]Shimoda Y, Ito H, Tanaka A. Conversion of chlorophyll b to chlorophyll a precedes magnesium dechelation for protection against necrosis in Arabidopsis. Plant J, 2012, 72: 501–511[40]Hideg E, Kalai T, Kos P B, Asada K, Hideg K. Singlet oxygen in plants—its significance and possible detection with double (fluorescent and spin) indicator reagents. Photochem Photobiol, 2006, 82: 1211–1218[41]Manjunatha G, Lokesh V, Neelwarne B. Nitric oxide in fruit ripening: Trends and opportunities. Biotechnol Adv, 2010, 28: 489–499[42]Wang Y, Lin A, Loake G J, Chu C. H2O2-induced leaf cell death and the crosstalk of reactive nitric/oxygen species. J Integr Plant Biol, 2013, 55: 202–208[43]汪媛. 水稻叶片衰老过程生理变化及蛋白质降解与蛋白酶活性变化研究. 扬州大学硕士学位论文, 江苏扬州, 2010Wang Y. The Research of Physiological Changes, Protein Degradation and Protease Activity in the Process of Leaf Senescence in Rice. MS Dissertation of Yangzhou University, Yanzhou, China, 2010 (in Chinese with English abstract)[44]何秀英, 徐世平, 廖耀平, 毛兴学, 翁克难, 陈钊明, 陈粤汉, 肖万生. 水稻高压变异材料的SSR标记和叶片可溶性蛋白质含量分析. 中国水稻科学, 2003, 17: 373–375He X Y, Xu S P, Liao Y P, Mao X X, Weng K N, Chen Z M, Chen Y H, Xiao W S. SSR analysis and soluble protein content in leaf of rice mutants induced by high pressure. Chin J Rice Sci, 2003, 17: 373–375 (in Chinese with English abstract)[45]Wu X Y, Kuai B K, Jia J Z, Jing H C. Regulation of leaf senescence and crop genetic improvement. J Integr Plant Biol, 2012, 54: 936–952[46]Li Y, Chen L, Mu J, Zuo J. LESION SIMULATING DISEASE1 interacts with catalases to regulate hypersensitive cell death in Arabidopsis. Plant Physiol, 2013, 163: 1059–1070[47]Smykowski A, Zimmermann P, Zentgraf U. G-Box binding factor1 reduces CATALASE2 expression and regulates the onset of leaf senescence in Arabidopsis. Plant Physiol, 2010, 153: 1321–1331 |
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