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作物学报 ›› 2014, Vol. 40 ›› Issue (11): 1956-1963.doi: 10.3724/SP.J.1006.2014.01956

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

普通小麦-卵穗山羊草种质的菲利普孢囊线虫抗性

邢小萍,杨静,袁虹霞,张佳佳,李洪连*,刘文轩*   

  1. 河南农业大学/河南省粮食作物协同创新中心 / 小麦玉米作物学国家重点实验室,河南郑州450002
  • 收稿日期:2013-12-23 修回日期:2014-09-16 出版日期:2014-11-12 网络出版日期:2014-09-26
  • 通讯作者: 李洪连, E-mail: honglianli@sina.com; 刘文轩, E-mail: wxliu2003@hotmail.com
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项基金(200903040), 国家高技术研究发展计划(863计划)项目(2012AA101105)和国家“十二五”粮食丰产科技工程项目(2012BAD04B07)资助。

Resistance to Heterodera filipjevi in Triticum aestivum?Aegilops geniculata Germplasm

XING Xiao-Ping,YANG Jing,YUAN Hong-Xia,ZHANG Jia-Jia,LI Hong-Lian*,LIU Wen-Xuan*   

  1. Henan Agricultural University / Collaborative Innovation Center of Henan Grain Crops / National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002, China
  • Received:2013-12-23 Revised:2014-09-16 Published:2014-11-12 Published online:2014-09-26

摘要:

菲利普孢囊线虫(Heterodera filipjevi)是我国黄淮麦区新发现的一种病原线虫,但在小麦属中缺乏有效抗源。为从小麦亲缘种属中发掘抗病资源,采用室内接种平均单株雌虫鉴定法,从34份卵穗山羊草(Aegilops geniculataRoth)材料中筛选出6份抗H. filipjevi的种质材料,其中PI542187表现高抗,PI564186、PI573396、PI374365、PI361880和PI374365表现抗病。对中国春–卵穗山羊草染色体附加系抗性鉴定发现,卵穗山羊草7Ug和5Mg附加系的单株白雌虫数明显低于中国春。连续两年又对17份小麦?卵穗山羊草5Mg-5D易位系材料的抗性鉴定发现,5Mg464、5Mg466和5Mg457抗性较好。

关键词: 卵穗山羊草, 菲利普孢囊线虫, 附加系, 易位系, 抗性鉴定

Abstract:

Heterodera filipjevi is a pathogenic nematode of wheat newly discovered in Huang-Huai Plain of China. However, germplasm resistant to H. filipjevi has been rarely found in genus Triticum. To mine resistance genes from wheat relative species, we identified the resistance to H. filipjevi in 34 accessions of Aegilops geniculata by inoculating H. filipjevi Xuchang population with mean of female number per plant method and relative resistance index method in greenhouse. Six candidates of germplasm were identified including PI542187 with high resistance and other five accessions (PI564186, PI573396, PI374365, PI361880, and PI374365) with resistance. A set of Chinese Spring (CS)?Ae. geniculata addition lines were used to chromosomally locate the resistance gene(s). Lines 7Ug and 5Mg showed obviously fewer females per plant than CS. In two consecutive years, 17 CS?Ae.geniculata 5Mg-5D translocation lines were further tested for resistance against H. filipjevi. Lines 5Mg464, 5Mg466, and 5Mg457 exhibited resistance to H. filipjevi.

Key words: Aegilops geniculata, Heterodera filipjevi, Additional line, Translocation line, Resistance identification

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