含Pm21基因的次级易位创制及鉴定

doi:10.3724/SP.J.1006.2023.21082

作物学报 ›› 2023, Vol. 49 ›› Issue (10): 2603-2612.doi: 10.3724/SP.J.1006.2023.21082

• 作物遗传育种·种质资源·分子遗传学 • 下一篇

含Pm21基因的次级易位创制及鉴定

张蓝月¹(), 罗江陶²(), 范超兰¹, 李亚洲¹, 姜博¹, 陈雪¹, 陈雪姣¹, 袁中伟¹, 甯顺腙¹, 张连全³, 刘登才³(), 郝明¹()

¹四川农业大学小麦研究所, 四川温江 611130
²四川省农业科学院作物研究所, 四川锦江 610066
³西南作物基因资源发掘与利用国家重点实验室, 四川温江 611130

收稿日期:2022-12-12 接受日期:2023-02-21 出版日期:2023-10-12 网络出版日期:2023-03-06
通讯作者: 郝明, E-mail: haomingluo@foxmail.com; 刘登才, E-mail: dcliu7@sicau.edu.cn
作者简介:张蓝月, E-mail: 569395258@qq.com;罗江陶, E-mail: jtluohao@163.com**同等贡献
基金资助:
四川省科学技术项目(2022ZDZX0014);四川省科学技术项目(2022NSFSC1696);国家自然科学基金项目(31971884);国家自然科学基金项目(32172020)

Creation and analysis of secondary translocation harbouring gene Pm21

ZHANG Lan-Yue¹(), LUO Jiang-Tao²(), FAN Chao-Lan¹, LI Ya-Zhou¹, JIANG Bo¹, CHEN Xue¹, CHEN Xue-Jiao¹, YUAN Zhong-Wei¹, NING Shun-Zong¹, ZHANG Lian-Quan³, LIU Deng-Cai³(), HAO Ming¹()

¹Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China
²Crop Research Institute, Sichuan Academy of Agricultural Science, Jinjiang 610066, Sichuan, China
³State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China

Received:2022-12-12 Accepted:2023-02-21 Published:2023-10-12 Published online:2023-03-06
Contact: E-mail: haomingluo@foxmail.com; E-mail: dcliu7@sicau.edu.cn
About author:**Contributed equally to this study
Supported by:
Sichuan Science and Technology Program(2022ZDZX0014);Sichuan Science and Technology Program(2022NSFSC1696);National Natural Science Foundation of China(31971884);National Natural Science Foundation of China(32172020)

摘要/Abstract

摘要：

小麦-簇毛麦6VS.6AL易位携带抗白粉病基因Pm21, 对我国小麦抗白粉病育种做出了重要贡献。本文对162份四川小麦品种(系)的55K SNP芯片数据分析表明, 25份含6VS.6AL易位染色体, 占15.4%。重组位置和单倍型分析表明, 这些材料的6VS.6AL均为着丝点易位、具有单一来源。根据系谱分析, 92R178为最原始的供体材料。利用由ph1b诱导6VS/6AS部分同源重组形成的, 含Pm21基因的初级易位6VS-6AS.6AL和6AS-6VS.6AL为亲本, 创制了1个含Pm21基因、6VS片段大幅减小的6AS-6VS-6AS.6AL次级易位。根据中国春参考基因组, 该次级易位的2个重组位点, 分别位于6A染色体53.1~53.8 Mb和90.7~92.2 Mb之间, 易位片段大小在36.9~39.1 Mb之间。分子细胞学鉴定表明, ph1b诱导外源易位的同时, 小麦自身的内源染色体也发生了大量易位, 这影响易位系的遗传稳定和育种利用。为了解决这个问题, 建议将ph1b基因突变系以杂合的形式进行长期保存。同时, 在利用ph1b诱导小麦-外源易位的过程中, 尽量减少ph1b纯合状态下的繁殖世代, 以减少小麦内源染色体易位。育种利用时, 应尽快消除小麦内源易位。

关键词: 小麦, 簇毛麦, 白粉病, 6VS.6AL易位, Pm21基因, 小片段易位系

Abstract:

Wheat-Haynaldia villosa 6VS.6AL translocation harbouring the gene Pm21 has made a great contribution to powdery mildew resistance breeding in China. Based on the data of 55K SNP chip, 25 (15.4%) out of 162 Sichuan wheat varieties contained the translocation. In this study, recombination point and haplotype analysis on the 25 varieties showed that it was centric translocation. Combined with the pedigree information, 92R178 was the original donor of the 6VS.6AL translocation in these varieties. 6AS-6VS-6AS.6AL secondary recombinant containing Pm21 was generated by using primary recombinants 6VS-6AS.6AL and 6AS-6VS.6AL as the cross parents, which both formed by the induction of ph1b. The secondary recombinant had a much smaller 6VS chromatin than the primary recombinants. Based on the Chinese Spring reference genome, the crossover points of the secondary recombinant were located within 53.1-53.8 Mb and 90.7-92.2 Mb of chromosome 6A, with a 6VS fragment size about 36.9-39.1 Mb. Molecular cytological identification also detected the extensive recombinants among wheat endogenous homoeologs induced by ph1b, which was not only disadvantage for genetic stabilization of wheat-alien recombinants but also for wheat breeding. A proposed solution to reduce endogenous recombinants was to preserve the ph1b mutant line in a heterozygous condition and reduce the selfing times during the development of ph1b-mediated wheat-alien recombination. In breeding, it is necessary to eliminate endogenous recombinants as soon as possible.

Key words: wheat, Haynaldia villosa, powdery mildew, 6VS.6AL translocation, Pm21 gene, small fragment translocation line

张蓝月, 罗江陶, 范超兰, 李亚洲, 姜博, 陈雪, 陈雪姣, 袁中伟, 甯顺腙, 张连全, 刘登才, 郝明. 含Pm21基因的次级易位创制及鉴定[J]. 作物学报, 2023, 49(10): 2603-2612.

ZHANG Lan-Yue, LUO Jiang-Tao, FAN Chao-Lan, LI Ya-Zhou, JIANG Bo, CHEN Xue, CHEN Xue-Jiao, YUAN Zhong-Wei, NING Shun-Zong, ZHANG Lian-Quan, LIU Deng-Cai, HAO Ming. Creation and analysis of secondary translocation harbouring gene Pm21[J]. Acta Agronomica Sinica, 2023, 49(10): 2603-2612.

图/表 5

表1

25份携带6VS.6AL易位的四川小麦品种(系)"

品种(系)名 Accession name	选育单位 Origin	杂交组合 Pedigree	审定年份 Released year	细胞学* Cytological*
绵阳27 Mianyang 27	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	81-5/81-24	1997	/
绵阳28 Mianyang 28	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	T79350-1-4/Mianyang 11	1997	/
川麦36 Chuanmai 36	四川省农业科学院 Sichuan Academy of Agricultural Sciences	Milan/SW5193	2002	×
内麦8号 Neimai 8	内江市农业科学院 Neijiang Academy of Agricultural Sciences	Mianyang 26/92R178	2003	√
西科麦1号 Xikemai 1	西南科技大学 SouthWest University of Science and Technology	Mianyang 88-304/Mo-212	2003	×
良麦2号 Liangmai 2	四川农业大学 Sichuan Agricultural University	Mianyang 26/// (10-A/88-1643//Chuanyu 12)	2004	×
绵麦37 Mianmai 37	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	96EW37/Mianyang 90-100	2004	√
内麦9号 Neimai 9	内江市农业科学院 Neijiang Academy of Agricultural Sciences	Mianyang 26/92R178	2004	√
杏麦2号 Xingmai 2	内江市农业科学院 Neijiang Academy of Agricultural Sciences	Mianyang 26/92R178	2004	√
内麦11号 Neimai 11	内江市农业科学院 Neijiang Academy of Agricultural Sciences	Mianyang 26/92R178	2007	√
绵麦185 Mianmai 185	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	Mianyang 96-5/Liaochun 10	2008	√
内麦3416 Neimai 3416	内江市农业科学院 Neijiang Academy of Agricultural Sciences	R57/Ping 5	2010	/
绵麦228 Mianmai 228	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	1275-1/Nei 2938//99-1522	2011	×
绵麦51 Mianmai 51	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	1275-1/99-1522	2012	√
西科麦7号 Xikemai 7	西南科技大学 Southwest University of Science and Technology	Chuanyu 11/Mo444	2012	√
Y11-1741	四川农业大学 Sichuan Agricultural University	Zimai 1/Mianyang 2003-1	2012
绵麦1618 Mianmai 1618	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	1275-1//Nei 2938/99-1522	2013	√
西科麦8号 Xikemai 8	西南科技大学 Southwest University of Science and Technology	97-392/Yun 225747-5	2013	√
西科麦9号 Xikemai 9	西南科技大学 Southwest University of Science and Technology	Nei 4301/Mianyang 31	2014	√
宜麦9号 Yimai 9	宜宾市农业科学院 Yibin Academy of Agricultural Sciences	R59/Yi 97-24	2014	×
川麦92 Chuanmai 92	四川省农业科学院 Sichuan Academy of Agricultural Sciences	Neimai 8/Jian 3//Chuanmai 42	2015	/
绵麦112 Mianmai 112	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	Mian 06-367/99-1522	2015	/
内麦366 Neimai 366	内江市农业科学院 Neijiang Academy of Agricultural Sciences	Balandal88/Nei 4344	2015	/
国豪麦3号 Guohaomai 3	四川国豪种业 Sichuan Guohao Seeds Industry Co., Ltd.	1227-185/99-1522//99-1572	2016	√
绵麦285 Mianmai 285	绵阳市农业科学院 Mianyang Academy of Agricultural Sciences	1275-1/99-1522	2016	√

表1

图1

图2

图3

图4

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含Pm21基因的次级易位创制及鉴定

Creation and analysis of secondary translocation harbouring gene Pm21

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