作物学报 ›› 2015, Vol. 41 ›› Issue (11): 1682-1691.doi: 10.3724/SP.J.1006.2015.01682
冯露1,2,钟理2,3,陈丹丹4,马有志2,徐兆师2,李连城2,周永斌2,陈明2,*,张小红1,
FENG Lu1,2,ZHONG Li2,3,CHEN Dan-Dan4,MA You-Zhi2,XU Zhao-Shi2,LI Lian-Cheng2,ZHOU Yong-Bin2,CHEN Ming2,*,ZHANG Xiao-Hong1,*
摘要:
在植物生长、发育和胁迫响应等生物学过程中发挥重要作用。本研究通过序列比对,从谷子中克隆到亚基基因。进化树分析显示,基因在进化上属于亚族,与玉米亚基基因亲缘关系较近。表达谱分析结果显示,在高盐、茉莉酸甲酯、水杨酸和脱落酸处理下表达上调,在低温和低氮处理下表达下调。亚细胞定位分析表明定位于液泡膜上。遗传转化拟南芥的耐盐性鉴定表明,在盐处理条件下,转基因株系的种子萌发率、幼苗主根长、植株鲜重及存活率显著高于野生型的。与野生型拟南芥植株相比,过表达植株体内含量减少,体内相对含水量提高。此外,萌发试验结果显示,在种子萌发后期,过表达植株对更加敏感。研究表明,谷子可以显著提高拟南芥耐盐性,这可能与其正向调控信号途径以及减少植株体内积累和水分散失有关。
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