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作物学报 ›› 2016, Vol. 42 ›› Issue (11): 1577-1591.doi: 10.3724/SP.J.1006.2016.01577

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

棉花酰基辅酶A结合蛋白(ACBP)家族基因的发掘及在非生物胁迫抗性中的功能鉴定

秦朋飞,尚小光,宋健,郭旺珍*   

  1. 南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京 210095
  • 收稿日期:2016-02-29 修回日期:2016-07-11 出版日期:2016-11-12 网络出版日期:2016-08-11
  • 通讯作者: 郭旺珍, E-mail: moelab@njau.edu.cn, Tel: 025-84396523
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2015ZX08005-004)和江苏现代作物生产协同创新中心(No. 10)项目资助。

Genome-wide Identification of Acyl-CoA-Binding Protein (ACBP) Gene Family and Their Functional Analysis in Abiotic Stress Tolerance in Cotton

QIN Peng-Fei,SHANG Xiao-Guang,SONG Jian,GUO Wang-Zhen*   

  1. State Key Laboratory of Crop Genetics &Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2016-02-29 Revised:2016-07-11 Published:2016-11-12 Published online:2016-08-11
  • Contact: Guo Wangzhen, E-mail: moelab@njau.edu.cn, Tel: 025-84396523
  • Supported by:

    This program was financially supported in part by the National Transgenic Program (2011ZX08005-004) and Jiangsu Collaborative Innovation Center for Modern Crop Production project (No.10).

摘要:

酰基辅酶A结合蛋白(ACBP)家族基因在植物正常生长发育、响应逆境胁迫及生物膜修复等方面具有重要作用。本研究基于陆地棉(Gossypium hirsutum)遗传标准系TM-1基因组序列信息,鉴定并获得21个棉花ACBP家族基因成员的全序列和染色体定位等信息。聚类分析表明这21个基因分属于I~IV类。依据与拟南芥的同源性,将棉花ACBP家族基因命名为GhACBP1~GhACBP6等6大亚类。转录组分析表明,该家族基因在不同组织、不同发育时期表达差异较大。不同逆境诱导分析表明,GhACBP1、GhACBP3和GhACBP6显著受盐、旱、低温、高温逆境胁迫诱导,而GhACBP4和GhACBP5对逆境胁迫响应不强烈。进一步分析表明,GhACBP3和GhACBP6的表达受过氧化氢(H2O2)、水杨酸(SA)、茉莉酸(JA)、脱落酸(ABA)和乙烯(ET)的诱导。病毒诱导的基因沉默(VIGS)试验表明,沉默GhACBP3和GhACBP6亚类基因会降低棉花植株对干旱和盐的耐性。目标基因沉默后,植株干物质积累下降,株高变矮,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性降低,丙二醛(MDA)含量升高,表明GhACBP3和GhACBP6在棉花抗旱、耐盐中发挥作用。研究为利用ACBP家族基因进行棉花抗逆性研究及应用提供参考。

关键词: 棉花, ACBP基因家族, 结构, 表达, 干旱胁迫, 盐胁迫

Abstract:

Acyl-CoA-binding protein (ACBP) plays important roles in plant development, including normal growth, response to biotic/abiotic stress and membrane system repairing. However, the functions of ACBPs in cotton still remain largely unknown. Based on the G. hirsutum TM-1 genome database, we identified 21 ACBP genes and obtained their sequence information, chromosomal location. Phylogenetic analysis revealed that the 21 ACBP genes were classified into four groups. According to the nomenclature of A. thaliana homologous gene, the cotton ACBP genes were named as GhACBP1–GhACBP6 subclasses. Transcriptome data showed that the expression patterns of ACBP genes varied significantly in different cotton tissues. Further abiotic stresses treatment analysis showed that GhACBP1, GhACBP3, and GhACBP6 sub-class genes could be significantly induced by drought, salt, low temperature and high temperature treatments, while GhACBP4 and GhACBP5 sub-class genes could not. GhACBP3 and GhACBP6 could also be significantly induced by hydrogen peroxide (H2O2), salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), and ethylene (ET). Virus induced gene silencing (VIGS) results exhibited that down-regulation of GhACBP3 and GhACBP6 sub-class genes would significantly reduce the plant tolerance to drought and salt stresses. Compared with the control, the GhACBP3 and GhACBP6 silencing plants demonstrated significantly reduced plant dry matter, decreased plant height, lower superoxide dismutase (SOD) and peroxidase (POD) activities, and

Key words: Cotton, ACBP gene family, Structure, Expression, Drought stress, Salt stress

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