作物学报 ›› 2017, Vol. 43 ›› Issue (02): 218-225.doi: 10.3724/SP.J.1006.2017.00218

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇



  1. 广东省植物发育生物工程重点实验室,华南师范大学生命科学学院,广东广州 510631
  • 收稿日期:2016-04-02 修回日期:2016-09-18 出版日期:2017-02-12 网络出版日期:2016-09-28
  • 通讯作者: 李晓云, E-mail: xiaoyun5893@qq.com, Tel: 020-85211378; 李玲, E-mail: liling502@126.com, Tel: 020-85211378
  • 基金资助:

    本研究由国家自然科学基金项目(31471422, 31671600)资助。

Screening of AhHDA1 Interacting-Protein AhGLK and Characterization in Peanut (Arachis hypogaea L.)

LI Mei-Juan,SU Liang-Chen,LIU Shuai,LI Xiao-Yun*,LI Ling*   

  1. Guangdong Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou, 510631, China
  • Received:2016-04-02 Revised:2016-09-18 Published:2017-02-12 Published online:2016-09-28
  • Contact: 李晓云, E-mail: xiaoyun5893@qq.com, Tel: 020-85211378; 李玲, E-mail: liling502@126.com, Tel: 020-85211378
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31471422, 31671600).


通过酵母双杂交系统,以花生组蛋白去乙酰化酶AhHDA1为诱饵, 对花生cDNA文库筛选并分析互作蛋白的生物学特性。结果获得一个AhHDA1互作蛋白Arachis hypogaea L. Golden 2-like (AhGLK);体外荧光双分子试验证实,AhHDA1与AhGLK蛋白存在相互作用;利用生物信息学软件分析表明,AhGLK含有MYB保守域,其氨基酸序列与大豆(Glyine soja) GsGLK、拟南芥(Arabidopsis thaliana) AtGLK分别具有较高同源性;AhGLK定位在细胞核中并具有转录因子活性,主要在花生的叶片中表达;在30% PEG条件下,AhGLK表达下调。

关键词: 花生, AhHDA1, 蛋白互作, AhGLK, 转录活性


With AhHDA1 as bait, the peanut (Arachis hypogaea L.) cDNA library was screened and characteristics of the interacting-protein was analysed, getting a protein of Golden 2-like (AhGLK) interacted with AhHDA1 which was proved by yeast two-hybrid experiments, and further confirmed by bimolecular fluorescence complementation (BiFC) assay. Bioinformatics software analysis indicated that AhGLK contained a MYB conserved domain having high homology with GsGLK (Glyine soja) and AtGLK (Arabidopsis thaliana). AhGLK was found to be located in nucleus and testified as a transcriptional activation factor. Tissue specific analysis showed that AhGLK accumulated in leaves mainly, and the expression was down-regulated by 30% PEG treatment. The results lay a foundation for further study on AhGLK, which might be involved in drought stress resistance of peanut.

Key words: Peanut, AhHDA1, Interacting protein, AhGLK, Transcriptional activity

[1] Zhu J K. Salt and drought stress signal transduction in plants. Ann Rev Plant Biol, 2002, 53: 247–273
[2] Loidl P. A plant dialect of the histone language. Trends Plant Sci, 2004, 9: 80–90
[3] Fong P M, Tian L, Chen Z J. Arabidopsis thaliana histone deacetylase 1 (AtHD1) is localized in euchromatic regions and demonstrates histone deacetylase activity in vitro. Cell Res, 2006, 16: 479–488
[4] Zhong X, Zhang H, Zhao Y, Sun Q, Hu Y F, Peng H, Zhou D X. The rice NAD(+)-dependent histone deacetylase OsSRT1 targets preferentially to stressand metabolism- related genes and transposable elements. PLoS One, 2013, 8(6): p.e66807
[5] Ma X J, Lv S B, Zhang C, Yang C P. Histone deacetylases and their functions in plants. Plant Cell Rep, 2013, 32: 465–478
[6] Devoto A, Nieto-Rostro M, Xie D X, Ellis C, Harmston R, Patrick E, Davis J, Sherratt L, Coleman M, Turner J. COI1 links jasmonate signalling and fertility to the SCF ubiquitinligase complex in Arabidopsis. Plant J, 2002, 32: 457–466
[7] Tanaka M, Kikuchi A, Kamada H, Kamada H. The Arabidopsis histonedeacetylases HDA6 and HDA19 contribute to the repression of embryonic properties after germination. Plant Physiol, 2008, 146: 149–161
[8] Zhou C H, Zhang L, Duan J, Miki B, Wu K Q. HISTONE DEACETYLASE19 is involved in jasmonic acid and ethylene signaling of pathogen response in Arabidopsis. Plant Cell, 2005, 17: 1196–1204
[9] Cigliano R A, Cremona G, Paparo R, Termolino P, Perrella G, Gutzat R, Consiglio M F, Conicella C. Histone deacetylase AtHDA7 is required for female gametophyte and embryo development in Arabidopsis. Plant Physiol, 2013, 163, 431–440
[10] Liu C, Li L C, Chen W Q, Chen X, Xu Z H, Bai S N. HDA18 affects cell fate in Arabidopsis root epidermis via histone acetylation at four kinase genes. Plant Cell, 2013, 25: 257–269
[11] Martijn V Z, Christian Z, Zhi W, Christina P, Annaick C, Li Y, Noortje G K, Liu Y X, Soppe W J. HISTONE DEACETYLASE 9 represses seedling traits in Arabidopsis thaliana dry seeds. Plant J, 2014, 80: 475–488
[12] Zhao L, Wang P, Hou H, Zhang H, Wang Y, Yan S, Huang Y, Li H, Tan J, Hu A, Gao F, Zhang Q, Li Y, Zhou H, Zhang W, Li L. Transcriptional regulation of cell cycle genes in response to abiotic stresses correlates with dynamic changes in histone modifications in maize. PLoS One, 2014, 9: e106070
[13] Li X Y, Lu J B, Liu S, Liu X, Lin Y Y, Li L. Identification of rapidly induced genes in the response of peanut (Arachis hypogaea) to water deficit and abscisic acid. BMC Biotechnol, 2014, 14: 116–119
[14] Su L C, Deng B, Liu S, Li L M, Hu B, Zhong Y T, Li L. Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea. Front Plant Sci, 2015, 6: 512-522
[15] Santos-Rosa H, Schneider R, Bannister A J, Sherriff J, Bernstein B E, Emre N C, Schreiber S L, Mellor J, Kouzarides T. Active genes are tri-methylated at K4 of histone H3. Nature, 2002, 419: 407–411
[16] Riechmann J L, Heard J, Martin G, Reuber L, Jiang C Z, Keddie J, Adam L, Pineda O, Ratcliffe O J, Samaha R R, Creelman R, Broun P, Zhang J Z, Ghandehair D, Sherman B K, Yu G L. Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science, 2000, 290: 2105–2110
[17] Kang J, Yu H, Tian C, Zhou W, Li C, Liao Y, Liu D. Suppression of photosynthetic gene expression in roots is required for sustained root growth under phosphate deficiency. Plant Physiol, 2014, 165: 1156–1170
[18] Wang P, Fouracre J, Kelly S, Karki S, Gowik U, Aubry S, Shaw M K, Westhoff P, Slamet-Loedin I H, Quick W P, Hibberd J M, Langdale J A. Evolution of GOLDEN2-LIKE gene function in C3 and C4plants. Planta, 2012, 237: 481–495
[19] Nguyen C V, Vrebalov J T, Gapper N E, Yi Z, silin Z, Zhang J F, James J, Giovannoni. Tomato GOLDEN2-LIKE transcription factors reveal molecular gradients that function during fruit development and ripening. Plant Cell, 2014, 26: 585–601
[20] Fitter D W, Martin M J, Copley D J, Scotland R W, Langdale J A. GLK gene pairs regulate chloroplast development in diverse plant species. Plant J, 2002, 31: 713–727
[21] Murmu J, Wilton M, Allard G, Pandeya R, Desveaux D, Singh J, Subramaniam R. Arabidopsis GOLDEN2-LIKE (GLK) transcription factors activate jasmonic acid (JA)-dependent disease susceptibility to the biotrophic pathogen Hyaloperonospora arabidopsidis, as well as JA-independent plant immunity against the necrotrophic pathogen Botrytis cinerea. Mol Plant Pathol, 2014, 15: 174–184
[22] Rauf M, Arif M, Dortay H, Matallana-Ramirez L P, Waters M T, Nam H G, Lim P O, Mueller-Roeber B, Balazadeh S. ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription. Embo Reports, 2013, 14: 382–388
[23] Cress W D, Seto E. Histone deacetylases, transcriptional control, and cancer. J Cell Physiol, 2000, 184: 1–16
[24] Sarah F, Thomashow M F. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell, 2002, 14: 1675–1690
[25] Bunch N L, Spasojevic M, Shprits Y Y, Gu X, Foust F. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J, 2002, 31: 279–292

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