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作物学报

作物学报 ›› 2006, Vol. 32 ›› Issue (12): 1909-1912.

• 研究简报 • 上一篇    下一篇

高粱A3细胞质雄性不育发生的细胞学观察及分析

仪治本1;梁小红1;赵威军2;崔贵梅3;孙毅3;崔丽霞1   

  1. 1中北大学环境工程系, 山西太原030051；2山西省农业科学院高粱研究所, 山西晋中030601；3山西省农业生物技术研究中心, 山西太原030031
  • 收稿日期:2006-02-23 修回日期:1900-01-01 出版日期:2006-12-12 网络出版日期:2006-12-12
  • 通讯作者: 仪治本

Cytolgical Observation and Analyses of A3 Cytoplasmic Male Sterility in Sorghum

YI Zhi-Ben1,LIANG Xiao-Hong1,ZHAO Wei-Jun2,CUI Gui-Mei3,SUN Yi3,CUI Li-Xia1   

  1. 1 Department of Environment Engineering, North University of China, Taiyuan 030051, Shanxi; 2 Sorghum Institute, Shanxi Academy of Agricultural Sciences, Jinzhong 030601, Shanxi; 3 Shanxi Agri-Biotechnology Research Center, Taiyuan 030031, Shanxi, China
  • Received:2006-02-23 Revised:1900-01-01 Published:2006-12-12 Published online:2006-12-12
  • Contact: YI Zhi-Ben

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1014

被引次数

23

摘要： 以不育系A3晋粱5号和相应保持系B3晋粱5号为材料，对A3不育系花药发育及雄配子形成过程进行了细胞学观察和分析。结果表明，与A1、A2型CMS不同，A3 CMS孢母细胞减数分裂正常，从四分体形成到幼龄花粉粒发育阶段都未观察到不同于B3晋粱5号的异常现象。A3晋粱5号花药经石蜡包埋切片观察，在造孢细胞期、减数分裂前间期及减数分裂期，均未观察到异常现象，不育系花粉母细胞能完成正常的减数分裂过程，四分体正常游离，绒毡层发育正常。但在成熟的花药中，观察到所有的花粉粒皱缩、凹陷、变形。在开花前约1周内的花粉粒成熟期，不育系花粉粒蔗糖—淀粉代谢途径受阻，花粉粒壁上无淀粉粒沉积。A3 CMS小孢子败育发生在小孢子形成晚期的花粉粒成熟期。

关键词: 高粱, A3细胞质雄性不育, 减数分裂

Abstract:

A3 male sterile cytoplasm in sorghum (Sorghum bicolor L. Moench) is from IS1112C. Among the seven kinds of cytoplasmic male sterility (CMS) in sorghum, the sterility in A3 CMS is most stable, and very difficult to restore. In this study A3 male sterile line Jingliang 5 and corresponding maintainer B3 Jinliang 5 were used to study the process of A3 CMS anther development and analyze the chromosomal behaviors of meiosis during microspore formation, in order to understand the basis of A3 CMS genesis. The results showed that differing from that in A1 and A2 CMS, the meiosis of pollen mother cell (PMC) in A3 Jinliang 5 was completed normally. No abnormal meiotic behaviors were observed compared to its corresponding maintainer B3 Jinliang 5. In sporogenous cell stage, premeiotic interphase and meiosis stage, no abnormal phenomena were found. Tetrad separated normally in paraffin-embedded A3 male sterile line anther section observation. But in pollen mature stage deformed pollens were observed and it was in this stage, i.e. about 7 days before anthesis, the normal development of microspores was affected, sucrose-starch metabolic pathway was blocked and no starch particles were deposited on intine of A3 CMS pollens, leading to unviable pollen formation.

Key words: Sorghum, A3 CMS, Meiosis

中图分类号: 

  • S514
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