关键词: 耐盐基因, 小麦, 丝氨酸/苏氨酸蛋白激酶, cDNA末端快速扩增, 基因克隆

Abstract:

The full-length cDNA sequence of TaSTK gene contained 1 958 bp was obtained from the salt-resistant wheat (Triticum aestivum) mutant RH8706-49 by RACE method. The coding sequence was 1 431 bp and by which 476 amino acids were encoded. The genome sequence of coding location contained 4 095 bp and 5 extrons. The cDNA sequence and genome sequence of TaSTK gene were submitted to GenBank (Accession No. QD103756, DQ341377). According to the BLAST results, the peptide encoded by this gene showed high homology to serine/threonine protein kinases (S_TKc, SPS1), tyrosine kinase (TyrKc) and protein kinase (Pkinase). Northern blotting results showed that TaSTK was a salt-induced gene in wheat. Under salt stress, the expression of TaSTK was elevated more strongly in the salt-tolerant line RH8706-49 than in the salt-sensitive line H8706-34. At the same time, the hybridization signals were all very weak, indicating that TaSTK belongs to the low expression gene in the wheat seedling tissue.

Key words: Salt-resistance gene, Wheat, Serine-threonine protein kinase, RACE, Gene cloning