关键词: 中间偃麦草, 白粉病, SGT1, 基因克隆, 小麦, 抗病性鉴定

Abstract:

To understand the role of SGT1 gene in wheat (Triticum aestivum L.) diseases resistance, we used RT-PCR and RACE methods to isolate SGT1 genes from Thinopyrum intermedium. The SGT1 was named TiSGT1. The deduced TiSGT1 protein possesses the typical domains of known SGT1 proteins and shows highly homology with barley SGT1 protein. A highly efficiency expression vector of TiSGT1 was constructed by genetic recombination, and transformed into the wheat cultivar ‘Yangmai 12’ by biolistic particle method. Transformation plants were analyzed by PCR, Southern hybridization, and RT-PCR methods. The results showed that the TiSGT1 gene could be inherited from T0 to T2 generations, and could be expressed. The results of disease resistance test indicated that the over-expression of SGT1 in the transgenic wheat enhanced resistance to barley yellow dwarf virus and powdery mildew, and TiSGT1 could be used as a potential gene resource for improving broad spectrum resistance of wheat.

Key words:

Thinopyrum intermedium, Powdery mildew, SGT1, Gene cloning, Wheat, Disease resistance test