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作物学报 ›› 2008, Vol. 34 ›› Issue (04): 539-544.doi: 10.3724/SP.J.1006.2008.00539

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

利用“永久F2”群体定位抗赤霉病QTL

田大刚;林峰;张彩琴;张政值;薛树林;曹勇;李春军;马正强*   

  1. 应用植物基因组学实验室和南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京210095
  • 收稿日期:2007-11-14 修回日期:1900-01-01 出版日期:2008-04-12 网络出版日期:2008-04-12
  • 通讯作者: 马正强

Mapping QTLs Associated with Resistance to Fusarium Head Blight Using an “Immortalized F2” Population

TIAN Da-Gang,LIN Feng,ZHANG Cai-Qin,ZHANG Zheng-Zhi, XUE Shu-Lin,CAO Yong,LI Chun-Jun,MA Zheng-Qiang *   

  1. Laboratory of Applied Plant Genomics and the National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
  • Received:2007-11-14 Revised:1900-01-01 Published:2008-04-12 Published online:2008-04-12
  • Contact: MA Zheng-Qiang

摘要:

为了研究抗赤霉病侵染性的遗传, 利用感赤霉病品种南大2419和抗赤霉病品种望水白杂交单粒传获得的重组自交系群体132个株系间的随机配对组合, 构建了一个包含198个株系的“永久F2”群体。通过两年抗侵染田间试验和QTL作图, 定位了6个抗侵染QTL, 其中抗性等位位点源于望水白的Qfhi.nau-4BQfhi.nau-5A以及源于南大2419的Qfhi.nau-2B的效应较为稳定。Qfhi.nau-4BQfhi.nau-5A的效应较大且以加性效应为主, 前者存在部分显性基因效应。此外, 还检测到4对显著的互作位点。这些结果进一步说明赤霉病抗性遗传的复杂性, 同时也表明在利用望水白进行抗赤霉病育种时早代选择抗侵染性是可行的。

关键词: 赤霉病, 望水白, “永久F2”群体, 类型I抗性

Abstract: Fusarium head blight (FHB) or scab disease, caused by Gibberrella zeae (Schw.) Petch, is a serious disease in many wheat (Triticum aestivum L.) growing regions worldwide. To study the inheritance of scab resistance against fungal penetration (type I resistance), an 198 lines “immortalized F2”population was constructed by crossing between recombinant inbred lines chosen by random permutation of 132 RILs from the RIL population derived from the cross between scab-susceptible cultivar Nanda 2419 and scab-resistant cultivar Wangshuibai. The population were then evaluated for percentage of infected spikes (PIS) across two years and six chromosome regions were detected for their association with type I resistance through interval mapping, among which Qfhi.nau-4B and Qfhi.nau-5A with the resistance alleles originated from Wangshuibai and Qfhi.nau-2B with the resistance allele from Nanda 2419 were consistently detected. Qfhi.nau-4B and Qfhi.nau-5A had the largest effects among the detected QTLs and both showed mainly the additive allelic effects, the former also had partial dominance. In addition, four pairs of significant interaction loci were identified. These results further demonstrate that wheat scab resistance are under complex genetic control and also imply that early generation selection for type I resistance in scab resistance breeding is feasible with Wangshuibai as the parent.

Key words: Fusarium head blight, Wangshuibai, “Immortalized F2” population, Type I resistance

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