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作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1806-1811.doi: 10.3724/SP.J.1006.2009.01806

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦地方品种小白冬麦抗白粉病基因分子标记

薛飞1,2，翟雯雯1,**，段霞瑜1,*，周益林1，吉万全2   

  1. 1中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室，北京100193；2西北农林科技大学农学院，陕西杨凌712100
  • 收稿日期:2009-03-19 修回日期:2009-04-23 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 段霞瑜, Email: xyduan@ippcaas.cn
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2006CB100202），国际科技支撑计划项目（2006BAD08A05），公益性行业科研专项（3-15）资助。

Microsatellite Mapping of Powdery Mildew Resistance Gene in Wheat Landrace Xiaobaidong

XUE Fei1,2,ZHAI Wen-Wen1,**,DUAN Xia-Yu1,*,ZHOU Yi-Lin1,JI Wan-Quan2   

  1. 1State Key Laboratory of Biology for Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;2College of Agrionomy,Northwest A&F University,Yangling 712100,China
  • Received:2009-03-19 Revised:2009-04-23 Published:2009-10-12 Published online:2009-08-07
  • Contact: DUAN Xia-Yu, Email: xyduan@ippcaas.cn

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1848

被引次数

45

摘要：

小麦农家品种小白冬麦对小麦白粉病具有良好抗性，对病原菌拥有较广的抗谱，并与其他已知抗白粉病基因的抗谱不同，遗传分析证实小白冬麦的苗期抗性由一个隐性抗白粉病基因控制。为了寻找与小白冬麦所携带抗白粉病基因连锁的分子标记，采用小白冬麦和感病品种Chancellor(CC)正反交组合，在2个F2群体125和107个单株上进行验证。结果显示，抗白粉病基因mlxbd与引物Xgwm577、Xgwm1267等紧密连锁，通过中国春及其第7部分同源群缺体—四体系，双端体系和缺失系将其定位在7B染色体长臂末端区域(7BL-10，Bin 0.78~1.00), 利用与mlxbd最近的引物Xgwm577扩增23个含有已知抗白粉病基因的小麦品种，检测发现这个引物不能单独用于分子标记辅助选择育种。

关键词: 小麦, 小白冬麦, 白粉病, 抗病基因, SSR标记

Abstract:

Xiaobaidong, a Chinese landrace, is proved to be resistant to powdery mildew (Blumeria graminis DC f. sp. tritici) with wide spectrum of resistance to pathogen races, especially effective to current prevalent races. This variety shows a response pattern that is different from the variety possessing documented Pm genes after inoculation with 10 isolates of different virulence. Genetic analysis indicated that a single recessive gene was responsible for the powdery mildew resistance at seedling stage. To detect the adjacent molecular markers of the gene, we constructed two mapping populations derived from the reciprocal crosses between Xiaobaidong and a susceptible cultivar Chancellor with 125 and 107 individuals of the F2 generation. Seedlings of F1 and F2 plants were inoculated with isolate E09 of B. graminis DC f. sp. tritici for powdery mildew resistance identification. Using resistant and susceptible bulks, we identified the resistance gene mlxbd between simple sequence repeat (SSR) markers Xgwm577 and Xgwm1267 on chromosome 7. The gene mlxbd was located on the distal bin of chromosome 7BL-10 (Bin 0.78-1.00) by using the Chinese Spring nullisomic-tetrasomics, ditelosomics, and deletion lines. To verify the efficiency of the SSR markers that might be used in molecular marker-assistant selection (MAS), genome DNA of 23 wheat varieties with definite resistance genes were amplifiedby Xgwm577, the proximal marker of mlxbd. The result showed that Xgwm577 was able to differentiate resistance genes originated from Chinese varieties with Pm5a and Pm5b except PmH, however, resistance genes near Pm5 locus were failed to be identified with Xgwm577. This indicates that Xgwm577 should be combined with other markers in MAS.

Key words: Wheat, Xiabaidong, Powdery mildew, Resistance gene, SSR marker

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