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作物学报 ›› 2010, Vol. 36 ›› Issue (06): 1050-1054.doi: 10.3724/SP.J.1006.2010.01050

• 研究简报 • 上一篇    下一篇

一个新的水稻黄绿叶突变体的遗传分析与基因定位

李秀兰1,2,孙小秋2,王平荣2,周慧2,邓晓建2,3,*   

  1. 1曲阜师范大学生命科学学院，山东曲阜273165；2四川农业大学水稻研究所，四川成都611130；3四川农业大学西南作物基因资源与遗传改良教育部重点实验室，四川成都611130
  • 收稿日期:2010-01-06 修回日期:2010-03-01 出版日期:2010-06-12 网络出版日期:2010-04-14
  • 通讯作者: 邓晓建, E-mail: xjdeng2006@yahoo.com.cn
  • 作者简介:李秀兰, E-mail: lxljewel@163.com
  • 基金资助:

    本研究由国家自然科学基金项目（30770145，30771314）和曲阜师范大学科研基金项目（XJ0706）资助。

Genetic Analysis and Gene Mapping of a Novel Yellow-Green Leaf Mutant in Rice

LI Xiu-Lan1,2, SUN Xiao-Qiu2, WANG Ping-Rong2, ZHOU Hui2,DENG Xiao-Jian2,3,*
  

  1. 1College of Life Science,Qufu Normal University,Qufu 273165,china;2Rice Research Institute,Sichuan Agricultural University,Chengdu 611130,China;3Key Laboratory of Southwest Crop Genetic Resources and Improvement,Ministry of Education,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2010-01-06 Revised:2010-03-01 Published:2010-06-12 Published online:2010-04-14
  • Contact: DENG Xiao-Jian, E-mail: xjdeng2006@yahoo.com.cn
  • About author:LI Xiu-Lan, E-mail: lxljewel@163.com

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2259

被引次数

80

摘要：

通过化学诱变获得一份稳定遗传的水稻黄绿叶突变体D83。该突变体苗期植株呈黄绿色，分蘖期开始逐渐转为淡绿色。与野生型相比，突变体苗期叶绿素a、叶绿素b和类胡萝卜素含量分别下降45.03%、53.93%和39.56%，成熟期每穗着粒数减少9.45%，千粒重下降10.76%。对D83与正常绿色品种杂交F1、F2代的遗传分析表明，D83的突变性状由一对隐性核基因控制。以D83/浙福802 F2代作定位群体，应用分子标记将D83所携带的突变基因定位于水稻第2染色体短臂的SSR标记RM110附近，InDel标记Ch2-27和Ch2-32之间，该基因与这2个InDel标记的遗传距离分别为1.2 cM和2.3 cM。认为D83所携带的突变基因是一个新的水稻黄绿叶突变基因，暂命名为chl13(t)。

关键词: 水稻, 黄绿叶突变体, 遗传分析, 基因定位

Abstract:

An inherited stably yellow-green leaf mutant D83 was isolated by chemistry mutagenesis. Its whole plant exhibited yellow-green character at the seedling stage, and gradually changed into pea green from the tillering stage. Compared with its wild-type parent 10079, at the seedling stage, the contents of chlorophyll a, chlorophyll b and carotenoid decreased by 45.03%, 53.93% and 39.56%, at the maturation period, the number of spikelets per panicle reduced by 9.45%, the 1000-grain weight by decreased 10.76%, respectively. Genetic analysis of F1 and F2 generations of D83 crossed with green rice varieties showed that the yellow-green leaf character was controlled by one pair of recessive nuclear genes. Genetic mapping of the mutant gene was conducted by using molecular marker and F2 mapping population of D83/Zhefu802. The results suggested that the mutant gene of D83 was mapped on the short arm of rice chromosome 2, nearby SSR marker RM110, between InDel markers Ch2-27 and Ch2-32. The genetic distances from the target gene to two InDel markers were 1.2 cM and 2.3 cM, respectively. The mutant gene of D83 was considered to be a new yellow-green leaf gene in rice and designated tentatively as chl13(t).

Key words: Oryza sativa L., Yellow-green leaf mutant, Genetic analysis, Gene mapping

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