绿豆遗传连锁图谱的整合

doi:10.3724/SP.J.1006.2010.00932

摘要/Abstract

摘要：

利用绿豆及其近缘种的701对SSR引物对现有绿豆遗传连锁图谱进行补充，结果在高感豆象绿豆栽培种Berken和高抗豆象绿豆野生种ACC41两亲本间筛选到多态性SSR引物103对。群体分析后，结合其他分子数据，使用作图软件Mapmaker/Exp 3.0b，获得一张含有178个遗传标记和12个连锁群，总长1 822.9 cM、平均图距10.30 cM的新遗传连锁图谱，其中96个SSR标记，90个来自绿豆近缘种，RFLP标记76个、RAPD标记4个、STS标记2个；对32个绿豆、小豆共用SSR标记在遗传连锁图谱的分布分析发现，二个基因组间有一定程度的同源性，共用标记在连锁群上的排列顺序基本上一致，只有部分标记显示绿豆和小豆基因组在进化过程中发生了染色体重排；利用新图谱对ACC41的抗绿豆象主效基因重新定位，仍定位于I(9)连锁群，与其相邻分子标记的距离均小于8 cM，其中与左翼SSR标记C220的距离约2.7 cM，该标记来自菜豆的第9连锁群。与原图谱比较，新定位的抗性基因与其相邻标记的连锁更加紧密。

关键词: 绿豆, 遗传连锁图谱, SSR标记, 图谱比较, 抗豆象基因

Abstract:

A high-density genetic linkage map with informative markers is essential for plant genome analysis, including gene mapping, identification of quantitative trait locus (QTL), map-based cloning, and physical map construction. One genetic linkage map of mungbean (Vigna radiata, 2n=2x=22) for the recombinant inbred line population (RIL) derived from an intersubspecific cross between a highly bruchid-susceptible cultivar Berken and a highly bruchid-resistant wild type ACC41 (V. radiata subsp. sublobata) was established. A total of 103 polymorphic SSR markers were screened from 701 pairs markers for density-enhancement of the previous map. Along with other marker data, a new genetic linkage map was constructed by using Mapmaker/Exp 3.0b, with 178 markers, including 96 SSR among which 90 locus from mungbean closely related species, 76 RFLP, four RAPD and two STS, spanning 12 linkage groups, covering total length 1 822.9 cM of the mungbean genome, with an average marker interval distance of 10.30 cM. In the study, we determined the SSR markers transferability of close relatives of mungbean including adzuki bean (Vigna angularis L.), black gram (Vigna mungo L.), common bean(Phaseolus vulgaris), cowpea (V. unguiculata) into mungbean genome. A total of 597 pairs SSR primers were tested and about 65%, 72%, 42%, 30% SSR markers above species respectively were effectively amplified in mungbean, indicating a certain degree of homology between these five genomes, and 97 pairs of polymorphic SSR screened from closely related species can be effectively used in the molecular genetic study of mungbean. Then the new map was compared with the map published for Azuki bean (Vigna angularis,2n=2x=22, n=11) using 32 SSR markers. Most of markers’ order in the two linkage maps was found to be highly conserved. However, chromosome rearrangement was occurred in two genomes after they diverged. In the new map, the major bruchid resistant gene was still mapped on LG I(9) with distances of less than 8.0 cM to the flanking markers, among which, the distance to SSR marker C220 which came from the LG 9 of common bean was less than 3.0 cM. Compared with the previous map, the mew location of the resistance gene is more closely linked with adjacent markers.

Key words: Mungbean, SSR, Genetic linkage map, Comparative mapping, Bruchid resistant gene

赵丹,程须珍,王丽侠,王素华,马燕玲. 绿豆遗传连锁图谱的整合[J]. 作物学报, 2010, 36(06): 932-939.

DIAO Dan, CHENG Xu-Zhen, WANG Li-Xia, WANG Su-Hua, MA Yan-Ling. Integration of Mungbean(Vigna radiata) Genetic Linkage Map[J]. Acta Agron Sin, 2010, 36(06): 932-939.

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