作物学报 ›› 2010, Vol. 36 ›› Issue (06): 932-939.doi: 10.3724/SP.J.1006.2010.00932
赵丹,程须珍,王丽侠,王素华,马燕玲
DIAO Dan,CHENG Xu-Zhen,WANG Li-Xia,WANG Su-Hua,MA Yan-Ling
摘要:
利用绿豆及其近缘种的701对SSR引物对现有绿豆遗传连锁图谱进行补充,结果在高感豆象绿豆栽培种Berken和高抗豆象绿豆野生种ACC41两亲本间筛选到多态性SSR引物103对。群体分析后,结合其他分子数据,使用作图软件Mapmaker/Exp 3.0b,获得一张含有178个遗传标记和12个连锁群,总长1 822.9 cM、平均图距10.30 cM的新遗传连锁图谱,其中96个SSR标记,90个来自绿豆近缘种,RFLP标记76个、RAPD标记4个、STS标记2个;对32个绿豆、小豆共用SSR标记在遗传连锁图谱的分布分析发现,二个基因组间有一定程度的同源性,共用标记在连锁群上的排列顺序基本上一致,只有部分标记显示绿豆和小豆基因组在进化过程中发生了染色体重排;利用新图谱对ACC41的抗绿豆象主效基因重新定位,仍定位于I(9)连锁群,与其相邻分子标记的距离均小于8 cM,其中与左翼SSR标记C220的距离约2.7 cM,该标记来自菜豆的第9连锁群。与原图谱比较,新定位的抗性基因与其相邻标记的连锁更加紧密。
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