绿豆遗传连锁图谱的整合

doi:10.3724/SP.J.1006.2010.00932

摘要/Abstract

摘要：

利用绿豆及其近缘种的701对SSR引物对现有绿豆遗传连锁图谱进行补充，结果在高感豆象绿豆栽培种Berken和高抗豆象绿豆野生种ACC41两亲本间筛选到多态性SSR引物103对。群体分析后，结合其他分子数据，使用作图软件Mapmaker/Exp 3.0b，获得一张含有178个遗传标记和12个连锁群，总长1 822.9 cM、平均图距10.30 cM的新遗传连锁图谱，其中96个SSR标记，90个来自绿豆近缘种，RFLP标记76个、RAPD标记4个、STS标记2个；对32个绿豆、小豆共用SSR标记在遗传连锁图谱的分布分析发现，二个基因组间有一定程度的同源性，共用标记在连锁群上的排列顺序基本上一致，只有部分标记显示绿豆和小豆基因组在进化过程中发生了染色体重排；利用新图谱对ACC41的抗绿豆象主效基因重新定位，仍定位于I(9)连锁群，与其相邻分子标记的距离均小于8 cM，其中与左翼SSR标记C220的距离约2.7 cM，该标记来自菜豆的第9连锁群。与原图谱比较，新定位的抗性基因与其相邻标记的连锁更加紧密。

关键词: 绿豆, 遗传连锁图谱, SSR标记, 图谱比较, 抗豆象基因

Abstract:

A high-density genetic linkage map with informative markers is essential for plant genome analysis, including gene mapping, identification of quantitative trait locus (QTL), map-based cloning, and physical map construction. One genetic linkage map of mungbean (Vigna radiata, 2n=2x=22) for the recombinant inbred line population (RIL) derived from an intersubspecific cross between a highly bruchid-susceptible cultivar Berken and a highly bruchid-resistant wild type ACC41 (V. radiata subsp. sublobata) was established. A total of 103 polymorphic SSR markers were screened from 701 pairs markers for density-enhancement of the previous map. Along with other marker data, a new genetic linkage map was constructed by using Mapmaker/Exp 3.0b, with 178 markers, including 96 SSR among which 90 locus from mungbean closely related species, 76 RFLP, four RAPD and two STS, spanning 12 linkage groups, covering total length 1 822.9 cM of the mungbean genome, with an average marker interval distance of 10.30 cM. In the study, we determined the SSR markers transferability of close relatives of mungbean including adzuki bean (Vigna angularis L.), black gram (Vigna mungo L.), common bean(Phaseolus vulgaris), cowpea (V. unguiculata) into mungbean genome. A total of 597 pairs SSR primers were tested and about 65%, 72%, 42%, 30% SSR markers above species respectively were effectively amplified in mungbean, indicating a certain degree of homology between these five genomes, and 97 pairs of polymorphic SSR screened from closely related species can be effectively used in the molecular genetic study of mungbean. Then the new map was compared with the map published for Azuki bean (Vigna angularis,2n=2x=22, n=11) using 32 SSR markers. Most of markers’ order in the two linkage maps was found to be highly conserved. However, chromosome rearrangement was occurred in two genomes after they diverged. In the new map, the major bruchid resistant gene was still mapped on LG I(9) with distances of less than 8.0 cM to the flanking markers, among which, the distance to SSR marker C220 which came from the LG 9 of common bean was less than 3.0 cM. Compared with the previous map, the mew location of the resistance gene is more closely linked with adjacent markers.

Key words: Mungbean, SSR, Genetic linkage map, Comparative mapping, Bruchid resistant gene

赵丹,程须珍,王丽侠,王素华,马燕玲. 绿豆遗传连锁图谱的整合[J]. 作物学报, 2010, 36(06): 932-939.

DIAO Dan, CHENG Xu-Zhen, WANG Li-Xia, WANG Su-Hua, MA Yan-Ling. Integration of Mungbean(Vigna radiata) Genetic Linkage Map[J]. Acta Agron Sin, 2010, 36(06): 932-939.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2010.00932

https://zwxb.chinacrops.org/CN/Y2010/V36/I06/932

参考文献

[1]Lin R-F(林汝法), Chai Y(柴岩), Liao Q(廖琴), Sun S-X(孙世贤). Minor Crops in China(中国小杂粮). Beijing: Chinese Agriculture Press, 2002. pp 192-209 (in Chinese)
[2]Zheng Z-J(郑卓杰). Food Legumes in China (中国食用豆类学). Beijing: Chinese Agriculture Press, 1995. pp 141-166 (in Chinese)
[3]Menancio-Hautea D, Kumar L, Danesh D, Young N D. A Genome Map for Mungbean
[4]
[Vigna radiata(L.) Wilczek] Based on DNA Genetic Markers (2n = 2x = 22) In: O'Brien J S ed. Genetic Maps 1992, A Compilation of Linkage and Restriction Maps of Genetically Studied Organisms. NY, USA: Cold Spring Harbor Laboratory Press, 1993. pp 6259-6261
[5]Lambrides C J, Lawn R J, Godwin I D. Two genetic linkage maps of mungbean using RFLP and RAPD markers
[J].Austr J Agric Resourc
[6]Humphry M E, Konduri V, Lambrides C J, Magner T, McIntyre C L, Aitken E A B, Liu C J. Development of a mungbean (Vigna radiate L.) Wilczek] RFLP linkage map and its comparison with lablab (Lablab purpureus) reveal a high level of colinearity between the two genomes. Theor Appl Genet, 2002, 105: 160-166
[7]Sholihin, Hautea D M. Molecular mapping of drought resistance in mungbean
[8]
[Vigna radiata(L.) Wilczek]: 1. Linkage map in mungbean using AFLP markers. J Biotechnol Pertanian, 2002, 7: 17-24
[9]Chen H M, Liu C A, Kuo C G, Chien C M, Sun H C, Huang C C, Lin Y C , Ku H M. Development of a molecular marker for a bruchid (Callosobruchus chinensis L.) resistance gene in mungbean. Euphytica, 2007, 157: 113-22
[10]Klos K L E, Paz M M, Marek L F, Cregan P B, Shoemaker R C. Molecular markers useful for detecting resistance to brown stem rot in soybean
[J].Crop Sci
[11]Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation and characterization of seven tetranucleotide microsatellite loci in mungbean, Vigna radiata
[J].Mol Ecol Notes
[12]Kumar S V, Tan S G, Quah S C, Yusoff K. Isolation of microsatellite markers in mungbean, Vigna radiata
[J].Mol Ecol Notes
[13]Miyagi M, Humphry M, Ma Z Y, Lambrides C J, Bateson M, Liu C J. Construction of bacterial artificial chromosome libraries and their application in developing PCR-based markers closely linked to a major locus conditioning bruchid resistance in mungbean
[J].Theor Appl Genet
[14]Gwag J G, Chung J W, Chung H K, Lee J H, Ma K H, Dixit A, Park Y J, Cho E G, Kim T S, Lee S H. Characterization of new microsatellite markers in mungbean (Vigna radiata L)
[J].Mol Ecol Notes
[15]Liu C-Y(刘长友), Cheng X-Z(程须珍), Wang S-H(王素华), Wang L-X(王丽侠), Sun L(孙蕾), Mei L(梅丽), Xu N(徐宁). The screening of SSR and STS markers for genetic diversity analysis of mungbean. J Plant Genet Res (植物遗传资源学报), 2007, 8(3): 298-302 (in Chinese with English abstract)
[16]Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A. Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the Genus and other legume genera: Implications for the transferability of SSRs in plants. Mol Biol Evol, 1998, 15: 1275-1287
[17]Sharma R K, Gupta P, Sharma V, Sood A, Mohapatra T, Ahuja P S. Evaluation of rice and sugarcane SSR markers for phylogenetic and genetic diversity analyses in bamboo. Genome, 2008, 51: 91-103
[18]J Kingston R W. Berken-a new mungbean variety. Qld Agric J, 1975, 101: 659-661
[19]Lawn R J, Cottrel1. A Wild mungbean and its relatives in Australia. Biologist, 1988, 35: 267-273
[20]Rebetzke G J. Attributes of Potential Adaptive and Agronomic Significance in the Wild Mungbean (Vigna radiate L.) Wikzek ssp. Sublobata (Roxb.) Verdc.). Brisbane, Australia: MS Dissertation of University of Queensland, 1994
[21]Ji W(季伟), Wang L-X(王立新), Sun H(孙辉), Wang M-Y(王茅雁), Zhao C-P(赵昌平). Predigestion of wheat SSR analysis protocol. J Agric Biotechnol (农业生物技术学报), 2007, 15(5): 907-908 (in Chinese with English abstract)
[22]Mei L(梅丽), Wang S-H(王素华), Wang L-X(王丽侠), Liu C-Y(刘长友), Sun L(孙蕾), Xu N(徐宁), Liu C-J(刘春吉), Cheng X-Z(程须珍).Mapping of genes resistant to bruchid in mungbean using recombinant inbred lines population. Acta Agron Sin(作物学报), 2007, 33(10): 1601-1605(in Chinese with English abstract)
[23]Han O K, Kaga A, Isemura T, Wang X W, Tomooka N, Vaughan D A. A genetic linkage map for azuki bean
[24]em>Vigna angularis (Willd.) Ohwi & Ohashi]
[J].Theor Appl Genet
[25]Song X-L(宋宪亮), Sun X-Z(孙学振), Zhang T-Z(张天真). Segregation distortion and its effect on genetic mapping in plants. J Agric Biotechnol (农业生物技术学报), 2006, 14(2): 286-292 (in Chinese with English abstract)
[26]Li W-H(李卫华), Liu W(刘伟), You M-S(尤明山), Xu J(许杰), Liu C-L(刘春雷), Li B-Y(李保云), Liu G-T(刘广田). Construction of wheat molecular linkage map using different SSR markers and the polymorphism of the markers. J Triticeae Crops (麦类作物学报), 2007, 27(1): 1-6 (in Chinese with English abstract)
[27]Wang Z-L(王竹林), Liu S-D(刘曙东), Liu H-Y(刘惠远), He Z-H(何中虎), Xia X-C(夏先春), Chen X-M(陈新民). Genetic linkage map in ‘Bainong 64 × ‘Jingshuang 16 of wheat. Acta Bot Boreal-Occident Sin (西北植物学报), 2006, 26(5): 886-892 (in Chinese with English abstract)
[28]Fatokun C A, Menancio-Hautea D I, Danesh D, Young N D. Evidence for orthologous seed weight genes in cowpea and mungbean based on RFLP mapping. Genetics, 1992, 132: 841-846
[29]Pereira M G, Lee M. Identification of genomic regions affecting plant height in sorghum and maize. Theor Appl Genet, 1995, 90: 380-388
[30]Menancio-Hautea D, Fatokun C A, Kumar L, Danesh D, Young N D. Comparative genome analysis of mungbean (Vigna radiata L. Wilczek) and cowpea(V. unguiculata L.) Walpers using RFLP mapping data. Theor Appl Genet, 1993, 86: 797-810
[31]Boutin S R, Young N D, Olsen T C, Yu Z H, Shoemaker R C, Vallejos C E. Genome conservation among three legume genera detected with DNA markers. Genome, 1995, 38: 928-937
[32]Pereira M G, Lee M. Identification of genomic regions affecting plant height in shorghum and maize. Theor Appl Genet, 1995, 90: 380-388
[33]Lagercrantz U. Comparative mapping in Arabidopsis and Brassica, fine scale genome collinearity and congruence of genes controlling flowering time
[J].Plant J

相关文章 15

[1]	李诗晴, 王茜, 王素华, 张耀文, 王丽侠. 绿豆种质资源苗期耐盐性鉴定及相关基因发掘[J]. 作物学报, 2026, 52(2): 376-388.
[2]	邵顺伟, 陈卓, 兰振东, 蔡兴奎, 邹华芬, 李晨曦, 唐景华, 朱熙, 张彧, 董建科, 金辉, 宋波涛. 基于BSA-seq技术的块茎芽眼深度QTL定位分析[J]. 作物学报, 2025, 51(7): 1725-1735.
[3]	王浩辰, 王克晶, 韩娟, 李向华. 东南沿海短绒野大豆两种代表性生境自然种群的空间遗传结构特征:种群内取样策略研究[J]. 作物学报, 2025, 51(11): 2875-2885.
[4]	郑栋, 周仙莉, 滕长才, 侯万伟, 张红岩, 刘玉皎. 基于SSR标记的青海蚕豆品种亲缘关系分析与指纹图谱构建[J]. 作物学报, 2025, 51(1): 79-90.
[5]	匡博文, 韦妳, 刘金典, 陈美燕, 毛兴洁, 段维兴, 杨细平. 基于甘蔗及其近缘属参考基因组开发SSR标记及数据库[J]. 作物学报, 2025, 51(1): 103-116.
[6]	郭飞翔, 李春霞, 周爽, 郭彬彬, 张均, 马超. 绿豆R2R3-MYB转录因子家族鉴定及其类黄酮合成调控基因的筛选[J]. 作物学报, 2025, 51(1): 117-133.
[7]	宋梦媛, 郭中校, 苏禹霏, 邓昆鹏, 兰天娇, 程钰鑫, 包淑英, 王桂芳, 窦金光, 姜泽锴, 王明海, 徐宁. 一种绿豆柱头外露突变体的转录组分析[J]. 作物学报, 2024, 50(4): 957-968.
[8]	陈志凯, 周仙莉, 张红岩, 滕长才, 侯万伟. 320份蚕豆蛋白质含量的SSR关联分析[J]. 作物学报, 2024, 50(11): 2775-2786.
[9]	李赢, 刘海翠, 石吕, 石晓旭, 韩笑, 刘建, 魏亚凤. 江苏裸大麦种质资源遗传多样性和群体结构分析[J]. 作物学报, 2023, 49(10): 2687-2697.
[10]	怀园园, 张晟瑞, 武婷婷, 李静, 孙石, 韩天富, 李斌, 孙君明. 大豆主要营养品质性状相关分子标记的育种应用潜力评价[J]. 作物学报, 2022, 48(8): 1957-1976.
[11]	胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报, 2022, 48(2): 367-379.
[12]	田红丽, 赵紫薇, 杨扬, 范亚明, 班秀丽, 易红梅, 杨洪明, 刘少荣, 高玉倩, 刘亚维, 王凤格. 290个吉林省审定玉米品种SSR-DNA指纹构建及遗传多样性分析[J]. 作物学报, 2022, 48(12): 2994-3003.
[13]	刘玉玲, 张红岩, 滕长才, 周仙莉, 侯万伟. 蚕豆SSR标记遗传多样性及与淀粉含量的关联分析[J]. 作物学报, 2022, 48(11): 2786-2796.
[14]	马超, 冯雅岚, 吴姗薇, 张均, 郭彬彬, 熊瑛, 李春霞, 李友军. 鼓粒期遮光对黑绿豆种皮花青素积累及相关基因表达特性的影响[J]. 作物学报, 2022, 48(11): 2826-2839.
[15]	王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed