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作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1258-1264.doi: 10.3724/SP.J.1006.2010.01258

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻黑条矮缩病抗性资源的筛选和抗性QTL 的定位

王宝祥1,江玲1,**,陈亮明1,卢百关2,王琦1,黎光泉1,樊继伟2,程遐年1,翟虎渠3,徐大勇2,万建民1,3,*   

  1. 1南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心,江苏南京210095;2江苏徐淮地区连云港农业科学研究所,江苏连云港222006;3中国农业科学院作物科学研究所,北京100081
  • 收稿日期:2010-03-04 修回日期:2010-04-22 出版日期:2010-08-12 网络出版日期:2010-06-11
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2007AA10Z191,2006AA100101,2006AA10Z1A5),江苏省高技术招标项目(BE2009301-3,BE2008354)和江苏省三项工程项目资助.

Screening of Rice Resources against Rice Black-Streaked Dwarf Virus and Mapping of Resistant QTL

WANG Bao-Xiang1,JIANG Ling1**,CHEN Liang-Ming1,LU Bai-Guan2,WANG Qi1,LI Guang-Quan1,FAN Ji-Wei2,CHENG Xia-Nian1,ZHAI Hu-Qu3,XU Da-Yong2,WAN Jian-Min13*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement / Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Lianyungang Agricultural Science of Xuhuai Area , Lianyungang 222006, China; 3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-03-04 Revised:2010-04-22 Published:2010-08-12 Published online:2010-06-11

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2097

被引次数

42

摘要： 通过水稻黑条矮缩病的田间鉴定发现，分蘖盛期的病状最为显著，是病症观察的最佳时期。对江苏省311份粳稻品种进行重病区田间鉴定试验，未发现对黑条矮缩病毒(RBSDV)免疫的品种。江苏省目前正在推广的24个主栽水稻品种发病率在10.0%~30.0%之间，曾推广的287份粳稻品种中，71.5%的品种发病率在10.0%~29.0%之间，其余品种发病率在29%以上。来源于日本的粳稻品种Koshihikari的黑条矮缩病发病率相对较低，籼稻高产品种桂朝2号为感病品种。利用Koshihikari/桂朝2号RILs群体进行抗RBSDV的基因定位，结果在第3染色体上标记RM7–RM5748之间检测到1个抗黑条矮缩病的QTL，来自Koshihikari的等位基因增强了水稻黑条矮缩病的抗性，携带抗性位点的家系明显提高了对RBSDV的抗性。本研究结果将为研究水稻黑条矮缩病和水稻抗黑条矮缩病分子育种提供重要信息。

关键词: 水稻, 黑条矮缩病, 病状调查, 抗源筛选, QTL定位

Abstract: Rice black-streaked dwarf virus(RBSDV)disease become epidemic in Jiangsu and Zhejiang provinces. To screen resistant germplasms and discover new resistance genes/QTLs against RBSDV, we evaluated the resistance to RBSDV in 311 japonica cultivars in field test. The results showed that no cultivar was immune to RBSDV. The disease rate of 24 main japonica cultivars grown in Jiangsu province at present was 10.0%–29.0% and that of 71.5% cultivars in 287 japonicapopularized in Jiangsu beforewas between 10.0% and 30.0%. Furthermore, quantitative trait loci (QTL) analysis was conducted by using 162 recombinant inbred lines (RILs), which derived from a cross between Guichao 2, a susceptible indica variety, and Koshihikari, a japonica variety with resistance to RBSDV. RBSDV resistances were evaluated using natural infection methods by scoring the disease rating. One putative QTL (qRBSDV3) controlling RBSDV resistance was mapped between the marker RM7 and RM5748 on chromosome 3, which explained 17.1% of the total phenotypic variation with LOD score of 5.4. The positive resistant effect contributed from Koshihikari. Further analysis revealed that the lines harboring the alleles of qRBSDV3 exhibited significantly increased resistance to RBSDV. The results should be very useful for breeding new RBSDV-resistant cultivars by marker-assisted selection (MAS). cultivars

Key words: Rice, Rice black-streaked dwarf virus, Screening resistant germplasm, QTL mapping

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