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作物学报 ›› 2011, Vol. 37 ›› Issue (02): 249-254.doi: 10.3724/SP.J.1006.2011.00249

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

两种环境下甘蓝型油菜含油量差异的QTL分析

李超1,2,3,李波1,3,**,曲存民1,3,阎星颖1,3,付福友1,3,刘利钊1,3,谌利1,3,李加纳1,3,*   

  1. 1西南大学农学与生物科技学院，重庆 400716；2贵州省油料研究所，贵阳 550006；3重庆市油菜工程技术研究中心，重庆 400716
  • 收稿日期:2010-08-10 修回日期:2010-12-01 出版日期:2011-02-12 网络出版日期:2010-12-20
  • 通讯作者: 李加纳, E-mail: ljn1950@swu.edu.cn, Tel: 023-68251950
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2008AA10Z147), 国家科技支撑计划项目(2009BADA8B01), 国家自然科学基金项目(31071450)和贵州省农科院创新基金项目[黔农科合(创新基金)2010007号]资助。

QTL Analysis of Oil Content Difference in Two Environments in Brassica napus L.

LI Chao1,2,3,LI Bo1,3,**,QU Cun-Min1,3,YAN Xing-Ying1,3,FU Fu-You1,3,Liu Li-Zhao1,3,CHEN Li1,3,LI Jia-Na1,3,*   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China; 2 Institute of Oil Crops, Guiyang 550006, China; 3 Chongqing Engineering Research Center for Rapeseed, Chongqing 400716, China
  • Received:2010-08-10 Revised:2010-12-01 Published:2011-02-12 Published online:2010-12-20
  • Contact: 李加纳, E-mail: ljn1950@swu.edu.cn, Tel: 023-68251950

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摘要： 利用本实验室构建的遗传连锁图谱和复合区间作图法检测重组自交系GH06×P174(SWU-1)和GH06×中油821(SWU-2)群体在2个环境中含油量差值的QTL。以SWU-1群体在2个环境中检测到2个含油量差值QTL，分别位于2个不同的连锁群，单个QTL可解释表型变异的10.31%~12.45%；以SWU-2群体在2个环境中检测到3个含油量差值QTL，分别位于2个不同的连锁群，单个QTL可解释表型变异的6.60%~10.58%。分析结果表明，含油量受环境影响较大，差值的变异幅度达到0~18.66个百分点，变异系数达到58.24%，说明在油菜的油脂合成中，存在对环境敏感和钝感的基因。含油量差值QTL与2个环境中分别检测到的含油量QTL没有明显的连锁关系，初步分析说明对环境敏感或钝感的基因与油脂合成基因不是同一个系统。

关键词: 甘蓝型油菜, 含油量, 环境, 差值QTL

Abstract: According to the genetic linkage map established by our laboratory and composite interval mapping (CIM), the QTLs oil content difference between two environments were detected using the recombinant inbred lines (RIL) SWU-1 and SWU-2in Brassica napus L.two QTLs of the oil content difference were located in the two linkage groups, each of the QTLs explained 10.31%–12.45% of phenotypic variation in SWU-1; three QTLs of the oil content difference were located in the two linkage groups, each of them explained 6.60%–10.58% of phenotypic variation in SWU-2. The results showed that the oil content difference of the different genotypes was quite different, ranging from 0 to 18.66% and the coefficient of variation was up to 58.24%, which means there are different environment insensitive genes for oil content in the different genotypes. There wereno visiblelinkage relationship between the QTLs of oil content difference and the QTLs of oil content in two environments, which indicated that sensitive and insensitive genes do not have the same genetic expression system as the oil synthesis genes.

Key words: Brassica napus L., Oil content, Environment, Difference QTL

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