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作物学报 ›› 2012, Vol. 38 ›› Issue (06): 1003-1008.doi: 10.3724/SP.J.1006.2012.01003

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦抗秆锈病基因Sr33的微卫星标记

韩建东1,2,**，李伟华2,**，曹远银2,*，宫志远1，姚强1   

  1. 1山东省农业科学院农业资源与环境研究所，山东济南 250100；2沈阳农业大学植物免疫研究所，辽宁沈阳 110866
  • 收稿日期:2011-09-04 修回日期:2012-02-22 出版日期:2012-06-12 网络出版日期:2012-03-29
  • 通讯作者: 曹远银, E-mail: caoyy66@yahoo.com.cn; Tel: 13604000358
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(200903035)和国家自然科学基金项目(31171829)资助。

Microsatellite Markers Linked to Stem Rust Resistance Gene Sr33 in Wheat

HAN Jian-Dong1,2,**,LI Wei-Hua2,**,CAO Yuan-Yin2,*,GONG Zhi-Yuan1,YAO Qiang1   

  1. 1Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2Institute of Plant Immunology, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2011-09-04 Revised:2012-02-22 Published:2012-06-12 Published online:2012-03-29
  • Contact: 曹远银, E-mail: caoyy66@yahoo.com.cn; Tel: 13604000358

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摘要： 小麦抗秆锈病基因Sr33对强毒力小种Ug99和我国多数小麦秆锈菌小种具有良好抗性。以感病品种McNair701和来源于Tetra Canthatch/Triticum tauschii的单基因系Sr33为亲本，选用我国流行的小种34MKG，对作图群体161个F2单株及其F2:3家系进行抗性鉴定分析，利用分离群体集群分析法对位于小麦1D染色体上的57对微卫星引物进行扩增多态性筛选。获得2对在亲本及F2抗、感群体间揭示多态性的共显性标记 Xbarc152和Xcfd15，位于Sr33两侧，与目标基因的遗传距离分别为2.4 cM和2.1 cM。

关键词: 微卫星标记, 小麦秆锈病, Sr33, 基因定位

Abstract: Gene Sr33 confers resistance not only toUg99, but also to most Puccinia graminis f.sp. tritici races in China. A mapping population, consisting of 161 F2 plants and F2:3 families derived from the crossbetween susceptible wheat cultivar “McNair701” and a Tetra Canthatch/Triticum tauschii line carrying single Sr geneSr33, were inoculated with chinese prevalent race 34MKG to locate this resistance gene using microsatellite markers. A total of 57 microsatellite markers covering chromosome 1D were used in bulked segregation analysis (BSA) based on resistance identification at seedlings stage. Two codominant markers, Xbarc152 and Xcfd15, were obtained, which were located at both sides of Sr33. The genetic distances of Xbarc152 and Xcfd15 to the target gene were 2.4 cM and 2.1 cM respectively. These closely linked markers can be used in marker-assisted selection of wheat breeding against stem rust disease.

Key words: Microsatellite marker, Wheat stem rust, Sr33, Gene location

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