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作物学报 ›› 2012, Vol. 38 ›› Issue (09): 1716-1722.doi: 10.3724/SP.J.1006.2012.01716

• 研究简报 • 上一篇    下一篇

小麦多子房和单子房性状的差异蛋白质组学研究

王志军，马守才*，毕晓静，史秀秀，李清峰，韩芳，亓佳佳，王书平，张改生，牛娜   

  1. 西北农林科技大学国家杨凌农业生物技术育种中心 / 国家小麦改良中心杨凌分中心 / 小麦育种教育部工程研究中心 / 陕西省作物杂种优势研究与利用重点实验室, 陕西杨凌 712100
  • 收稿日期:2012-02-16 修回日期:2012-06-06 出版日期:2012-09-12 网络出版日期:2012-07-03
  • 通讯作者: 马守才, E-mail: mashoucai@sohu.com, Tel: 13991218803
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2009AA101102), 陕西省自然科学基金(2012JM3003)和西北农林科技大学2009年唐仲英育种基金资助。

Differential Proteomics on Multi-Ovary and Mono-Ovary Trait of Wheat

WANG Zhi-Jun,MA Shou-Cai*,BI Xiao-Jing,SHI Xiu-Xiu,LI Qing-Feng,HAN Fang,QI Jia-Jia,WANG Shu-Ping,ZHANG Gai-Sheng,NIU Na   

  1. Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center / Yangling Branch of State Wheat Improvement Center / Wheat Breeding Engineering Research Center, Ministry of Education / Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling 712100, China?
  • Received:2012-02-16 Revised:2012-06-06 Published:2012-09-12 Published online:2012-07-03
  • Contact: 马守才, E-mail: mashoucai@sohu.com, Tel: 13991218803

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摘要： 小麦多子房性状的蛋白质组学特性和形成机制及其在小麦杂种优势中的应用可能提供理论依据。为揭示以小麦单子房品系77(2)及其多子房近等基因系Mu77(2)为材料, 采用TCA-丙酮法提取穗分化至四分体时期的幼穗总蛋白, 并通过IEF/SDS-PAGE双向凝胶电泳分离, 获得了分辨率和重复性较好的蛋白质组差异图谱。在等电点4~7、分子量14.4~97.4 kD之间发现约450个肉眼可辨的蛋白点, 其中上调2倍以上且达到99%统计学显著水平的差异表达蛋白点30个。对6个特异性差异蛋白点作二级质谱(LC-MS/MS)分析, 结果表明它们是富含甘氨酸RNA结合蛋白(S1)、SGT1-1(S2)、HMG-I/Y(S3)、谷胱甘肽转移酶(S4)、果糖1,6-二磷酸醛缩酶(S5)和未知蛋白(S6)。这些蛋白质对DNA转录、蛋白质翻译、能量转换及代谢、抗逆防卫等生理生化过程起调控作用, 可能与小麦多子房性状的形成有关。

关键词: 小麦, 多子房, 二级质谱, 双向电泳

Abstract:  Muliti-ovary is a favorable trait that might be a solution for increasing the yield of hybrid seed in wheat. In this study, the differential proteins of panicles at tetrad stage were detected between the mono-ovary line 77(2) and its near-isogenic multi-ovary line Mu77(2) using SDS-PAGE method. Approximately 450 protein spots were observed in the 2-DE gels within the area of pH 4–7 and molecular weight 14.4–97.4 kD, of which 30 were identified as differential protein spots with at least double expression levels and 99% statisticalty significant difference. Six specific protein spots with differential expressions were then analyzed using LC-MS/MS method. The result showed that they are glycine-rich RNA-binding protein (S1), SGT1-1 (S2), HMG-I/Y protein (S3), glutathione transferase (S4), fructose-bisphosphate aldolase (S5), and unnamed protein (S6). These proteins are involved in the regulation of DNA transcription, protein translation, energy metabolism, signal transduction, plants defense, and other resistance function, and might be related to the formation of multi-ovary in wheat.

Key words: Wheat, Multi-ovary trait, LC-MS/MS, Two-dimensional electrophoresis

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