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作物学报 ›› 2013, Vol. 39 ›› Issue (02): 191-197.doi: 10.3724/SP.J.1006.2013.00191

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

普通小麦–大赖草易位系T7BS•7Lr#1S和T2AS•2AL-7Lr#1S的分子细胞遗传学鉴定

崔承齐1,王林生1,2,陈佩度1,*   

  1. 1南京农业大学/作物遗传改良与种质创新国家重点实验室/细胞遗传研究所,江苏南京 210095;2河南科技大学农学院,河南洛阳 471003
  • 收稿日期:2012-07-08 修回日期:2012-10-05 出版日期:2013-02-12 网络出版日期:2012-12-11
  • 通讯作者: 陈佩度, E-mail: pdchen@njau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(30871519), 国家重点基础研究发展计划(973计划)项目(2009CB118304)和江苏省优势学科建设项目资助。

Molecular and Cytogenetic Identification of Triticum aestivumLeymus racemosus Translocation Lines T7BS•7Lr#1S and T2AS•2AL-7Lr#1S

CUI Cheng-Qi1,WANG Lin-Sheng1,2,CHEN Pei-Du1,*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China;
    2 College of Agriculture, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2012-07-08 Revised:2012-10-05 Published:2013-02-12 Published online:2012-12-11
  • Contact: 陈佩度, E-mail: pdchen@njau.edu.cn

摘要:

大赖草7Lr#1S染色体上携带赤霉病抗性基因,将其导入普通小麦有助于增加赤霉病抗源多样性和选育抗赤霉病品种。利用染色体C-分带、荧光原位杂交和分子标记技术从普通小麦大赖草7Lr#1单体异附加系的花粉辐射后代中,选育出易位系T7BS·7Lr#1S (NAU639)T2AS·2AL-7Lr#1S (NAU640)。经连续3年大田、温室赤霉病接种鉴定,这2个易位系的赤霉病抗性均显著高于感病亲本中国春、感病对照绵阳8545和石麦4185,为赤霉病抗病育种提供了新的种质资源。

关键词: 普通小麦, 大赖草, 易位系, 分子细胞遗传学, 赤霉病抗性

Abstract:

A resistance gene against wheat scab is located on chromosome 7Lr#1S of Leymus racemosus, which is propitious to enriching scab resistance resources and resistant breeding in wheat (Triticum aestivum L.).The translocation lines NAU639 (T7BS·7Lr#1S) and NAU640 (T2AS·2AL-7Lr#1S) were selected from the plant progenies of irradiated pollens of monosomic alien addition line MA7Lr#1 and confirmed through chromosome FISH-GISH, C-banding, and molecular markers analyses. The two translocation lines had higher resistance to wheat scab than the susceptible parent “Chinese Spring” and the susceptible controls “Mianyang 8545” and “Shimai 4185” in a successive three-year test both in the greenhouse and field. These results showed the stable scab resistance in translocation lines NAU639 and NAU640 and feasibility to be used as a new source in wheat breeding resistant to scab disease.

Key words: Triticum aestivum, Leymus racemosus, Translocation line, Molecular and cytogenetics, Scab resistance

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