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作物学报 ›› 2013, Vol. 39 ›› Issue (03): 494-505.doi: 10.3724/SP.J.1006.2013.00494

• 耕作栽培·生理生化 • 上一篇    下一篇

后期水分亏缺与增施氮肥对杂交稻叶片光合功能的影响

吴自明,王竹青,李木英*,曾蕾,石庆华,潘晓华,谭雪明   

  1. 作物生理生态与遗传育种教育部重点实验室 / 江西省作物生理生态与遗传育种重点实验室 / 江西农业大学，江西南昌330045
  • 收稿日期:2012-08-06 修回日期:2012-11-16 出版日期:2013-03-12 网络出版日期:2013-01-04
  • 通讯作者: 李木英, E-mail: myli53@yahoo.com.cn, Tel: 0791-83813877
  • 基金资助:

    本研究由国家自然科学基金项目(30860137)，国家粮食丰产科技工程项目(2011BAD16B04)和江西省教育厅项目(GJJ09168)资助。

Effect of Water Shortage and Increasing Nitrogen Application on Photosynthetic Function of Different Hybrid Rice Combinations at Grain Filling Stage

WU Zi-Ming,WANG Zhu-Qing,LI Mu-Ying*,ZENG Lei,SHI Qing-Hua,PAN Xiao-Hua,TAN Xue-Ming   

  1. Key Laboratory of Crop Physiology Ecology and Genetic Breeding, Ministry of Education / Key Laboratory of Crop Physiology, Ecology and Genetic Breeding of Jiangxi Province / Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2012-08-06 Revised:2012-11-16 Published:2013-03-12 Published online:2013-01-04
  • Contact: 李木英, E-mail: myli53@yahoo.com.cn, Tel: 0791-83813877

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摘要：

为了探讨以水肥调控延长水稻结实期叶片功能的可能性，研究了早衰型和非早衰型杂交稻组合后期衰老过程中叶片光合功能对适度水分亏缺和增施氮肥的响应。结果表明，水稻灌浆期水分亏缺促使叶绿素和叶面积衰减加剧，加速Rubisco羧化活性和叶片光合功能衰退，叶片ZR (玉米素核苷)含量下降。对缺水较敏感的中优838、天优998和隆平601其叶绿素衰减率提高了9%~12%；光合速率各下降了19.55%、19.12%、12.73%；ZR含量、Rubisco羧化活性的降幅相对高于其余组合。在限水的同时增施氮肥明显缓解了水分亏缺胁迫的影响，但增氮效果组合间存在较大差异。叶绿素衰减率降低6.14%~27.10%；光合速率，隆平601、天优998、汕优63和中优838提高9.30%~15.81%，两优6326和C两优396提高3%~5%；Rubisco羧化活性，隆平601提高18.94%，中优838提高12.16%，其他组合提高3%~9%；增施氮肥提高了叶片ZR含量，中优838叶片ZR平均含量提高16.05%，C两优396和汕优63分别提高8.31%和8.16%，两优6326和隆平601分别提高4.90%和1.13%。相关分析表明，叶片ZR含量与叶光合速率和Rubisco活性呈极显著正相关，Rubisco羧化活性与光合速率和叶绿素含量极显著相关，叶片总糖含量和稻谷产量与光合速率相关显著和极显著。水稻灌浆期水分不足加速叶片衰老和光合功能衰退，在干旱胁迫时适量增施氮肥，是降低干旱胁迫危害、增强叶片光合功能、延长叶片功能期，防御早衰的有效措施。

关键词: 杂交稻, 衰老, 水分亏缺, 增氮, 光合功能

Abstract:

The response of photosynthetic function of leaves in aging process to water shortage and additional nitrogen application were studied using premature senescence type and non-premature senescence type of hybrid rice combinations. The results showed that chlorophyll content, leaf area, Rubisco activity and photosynthetic rate decreased, and ZR (zeatin riboside) content declined by water deficiency. There were significantly different responses to water shortage among different combinations. Under water stress, Zhongyou 838, Tianyou 998, and Longping 601 were more sensitive than others, and their chlorophyll decline rate increased by 9–12%, whereas photosynthetic rate decreased by 19.55%, 19.12%, and 12.73% respectively, and their ZR content, Rubisco activity dropped much more than those of other combinations, Under water shortage, additional nitrogen application rate could relieve the effect of water shortage stress, the decline rate of chlorophyll decreased by 6.14–27.10%, photosynthetic rate increased by 9.30–15.81% for Longping 601, Tianyou 998, Xianyou 63, and Zhongyou 838, and 3–5% for Liangyou 6326 and C Liangyou 396. And Rubisco activity was enhanced by 18.94% in Longping 601, 12.16% in Zhongyou 838, and 3–9% in other combinations. Additional nitrogen application increased ZR content by 16.05% in Zhongyou 838, 8.31% and 8.16% in C Liangyou 396 and Xianyou 63, and 4.90% and 1.13% in Liangyou 6326 and Longping 601.The correlation analysis showed that ZR content was positively correlated with, photosynthetic rate and Rubisco activity. Rubisco activity and photosynthetic rate had a positive correlation with chlorophyll content, and content of total sugars and yield were significantly correlated with photosynthetic rate.The conclusion was that water shortage speeds up the aging and the decline photosynthetic function decrease. Increasing moderate nitrogen fertilizer will delay the premature senescence of leaves caused by drought stress, enhance the photosynthetic function, and extend photosynthetic function period, suggesting that N nutrition is the most important factor for regulating rice leaf senescence.

Key words: Hybrid rice, Senescence, Water shortage, Additional nitrogen application, Photosynthetic function

[1]Wu W-M(吴伟明), Cheng S-H(程式华). Significance and prospects of breeding for root system in rice (Oryza sativa). Chin J Rice Sci (中国水稻科学), 2005, 19(2): 174–180 (in Chinese with English abstract)



[2]Zhai H-Q(翟虎渠), Cao S-Q(曹树青), Wan J-M(万建民), Lu W(陆巍), Zhang R-X(张荣铣), Li L-B(李良璧), Kuang T-Y(匡廷云), Min S-K(闵绍楷), Zhu D-F(朱德峰), Cheng S-H(程式华). Relationship between leaf photosynthetic function at grain filling stage and yield in super-high-yielding hybrid rice (Oryza sativa L.). Sci China (Ser. C) (中国科学•C辑), 2002, 32(3): 211–217 (in Chinese)



[3]Zhai R-R(翟荣荣), Feng Y(冯跃), Cao L-Y(曹立勇), Wu W-M(吴伟明), Cheng S-H(程式华). Advances of research on rice leaf senescence. China Rice(中国稻米), 2011, 17(1): 7–12 (in Chinese)



[4]Li M-Y(李木英), Shi Q-H(石庆华), Zheng W(郑伟), Pan X-H(潘晓华), Tan X-M(谭雪明). Preliminary research on relationship between leaves premature senescence characteristic and leaf N content, roots activity in hybrid rice during grain filling stage. Acat Agric Univ Jiangxiensis (江西农业大学学报), 2008, 30(5): 757–765 (in Chinese with English abstract)



[5]Li M-Y(李木英), Shi Q-H(石庆华), Zheng W(郑伟), Pan X-H(潘晓华), Tan X-M(谭雪明). Study on the leaves senescence type and influencing relationship factor in hybrid rice during grain filling stage. Acat Agric Univ Jiangxiensis (江西农业大学学报), 2010, 32(6): 1081–1088 (in Chinese with English abstract)



[6]Xu N-X(许乃霞), Yang Y-H(杨益花). Research on the relationship between the root activity and leaf ageing, photosynthetic rate of rice after heading stage. J Anhui Agric Sci (安徽农业科学), 2009, 37(5): 1919–1921 (in Chinese with English abstract)



[7]Cai Y-P(蔡永萍), Zuo Z-D(左震东), Yang Q-G(杨其光). The relation between activity of root system and flag leaf senescence and yield of hybrid-rice after blooming. Chin Agric Sci Bull (中国农学通报), 1998, 14(3): 17–20 (in Chinese with English abstract)



[8]Ou Z-Y(欧志英), Peng C-L(彭长连), Yang C-W(阳成伟), Lin G-Z (林桂珠), Duan J(段俊), Wen X(温学). High efficiency photosynthetic characteristic on flag leaves of super high-yield rice. J Trop Subtrop Bot (热带亚热带植物学报), 2003, 11(1): 1–6 (in Chinese with English abstract)



[9]Hua C(华春), Wang R-L(王仁雷), Wei J-C(魏锦城). Changes in the chloroplasts during leaf senescence in hybrid rice and three lines. J Nanjing Norm Univ (Nat Sci Edn) (南京师大学报•自然科学版), 1998, 12(1): 70–76 (in Chinese with English abstract)



[10]Mao W-H(毛伟华), Jiang D-A(蒋德安), Weng X-Y(翁晓燕), Lu Q(陆庆), Zhu W-D(朱卫东). The relationship between photosynthesis and Ribulose-1,5-bisphotosphate carboxylase oxygcenase in different rice cultivars. Acta Agric Zhejiangensis (浙江农业学报), 1999, 11(3): 114–118 (in Chinese with English abstract)



[11]Chen X-H(陈新红), Liu K(刘凯), Xu G-W(徐国伟), Wang Z-Q(王志琴), Yang J-C(杨建昌). Effects of nitrogen and soil moisture on photosynthetic characters of flag leaf, yield and quality during grain filling in rice. J Shanghai Jiaotong Univ (Agric Sci)(上海交通大学学报•农业科学版), 2004, 22(1): 46–52 (in Chinese with English abstract)



[12]Ookawa T, Naruoka Y, Sayama A, Hirasawa T. Cytokinin effects on Ribulose-1,5-bisphosphate carboxylase/oxygenase and nitrogen partitioning in rice during ripening. Crop Sci, 2004, 44: 2107–2115



[13]Imai K, Suzki Y, Mae T, Makino A. Changes in the synthesis of Rubisco in rice leaves in relation to senescence and N influx. Ann Bot, 2008, 101: 135–144



[14]Wang L F, Chen Y Y. Photosynthetic characterization at different senescence stages in an early senescence mutant of rice Oryza sativa L. Photosynthetica, 2011, 49: 140–144



[15]Weng X-Y(翁晓燕), Jiang D-A(蒋德安), Zhang F(张峰). Gene expression of key enzymes for photosynthesis during flag leaf senescence of rice after heading. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2002, 28(4): 311–316 (in Chinese with English abstract)



[16]Chen G-Y(陈根云). Guide to the Experiment of Modern Plant Physiology (现代植物生理指导). Beijing: Science Press, 1999. p 113 (in Chinese)



[17]Tong P(童平), Yang S-M(杨世民), Ma J(马均), Wu H-Z(吴合洲), Fu T-L(傅泰露), Li M(李敏), Wang M-T(王明田). Photosynthetic characteristics and dry matter accumulation of hybrid rice varieties under different light conditions. Chin J Appl Ecol (应用生态学报), 2008, 19(3): 505–511 (in Chinese with English abstract)



[18]Zhang Y-P(张玉屏), Zhu D-F(朱德峰), Lin X-Q(林贤青), Chen H-Z(陈惠哲). Effects of water stress on rice growth and yield at different growth stages. Agric Res Arid Areas (干旱地区农业研究), 2005, 23(2): 48–52 (in Chinese with English abstract)



[19]Lu W(陆巍). Studies on the Photosynthetic Function Decline of Rice (Oryza sativa L.) and the Changes of Activity and Structure of Rubisco. PhD Dissertation of Nanjing Agricultural University, 2000 (in Chinese with English abstract)



[20]Zhang R-X(张荣铣), Dai X-B(戴新宾), Xu X-M(许晓明), Cao S-Q(曹树青). Photosynthetic function of leaf and the potential photosynthetic productivity of crops. J Nanjing Norm Univ (Nat Sci Edn) (南京师大学报•自然科学版), 1999, 22(3): 376–386 (in Chinese with English abstract)



[21]Tian X-Y(田秀英). The recent reseach in ribulose 1,5-bisphosphate aboxylase/oxygenase. J Chongqing Teachers Coll (重庆师专学报), 2000, 19(3): 77–79 (in Chinese with English abstract)



[22]Hirotsu K, Makino A, Yokota S, Mae T. The photosynthetic properties of rice leaves treated with low temperature and high irradiance. Plant Cell Physiol, 2005, 46: 1377–1383



[23]Lu C-M(卢从明), Wang Z(王忠), Gao Y-Z(高煜珠). Injury of water stress to photosynthetic mechanism in rice. J Jiangsu Agric Coll (江苏农学院学报), 1993, 14 (2): 13–17 (in Chinese with English abstract)



[24]Wang H-Z (王贺正), Ma J(马均), Li X-Y(李旭毅), Zhang R-P(张荣萍). Effects of water stress on photosynthetic characteristics and protective enzyme system in rice during grain filling stage. Southwest China J Agric Sci (西南农业学报), 2009, 22(2): 290–294 (in Chinese with English abstract)



[25]Wei F(魏峰). Study on the Decline Process of Main Photosynthetic Parameters in Rice Flag Leaf. MS Thesis of Nanjing Agricultural University, 2003 (in Chinese with English abstract)



[26]Chen G(陈贵), Zhou Y(周毅), Guo S-H(郭世伟), Shen Q-R(沈其荣). The regulatory mechanism of different nitrogen form on photosynthetic efficiency of rice plants under water stress. Sci Agric Sin (中国农业科学), 2007, 40(10): 2162–2168 (in Chinese with English abstract)



[27]Zhao Q-Z(赵全志), Chen J-R(陈静蕊), Liu H(刘辉), Qiao J-F(乔江方), Gao T-M(高桐梅), Yang H-X(杨海霞), Wang J-H(王继红). Relationship between activities of nitrogen assimilation enzymes and leaf color of rice. Sci Agric Sin (中国农业科学), 2008, 41(9): 2607–2616 (in Chinese with English abstract)



[28]Ding C, You J, Wang S, Liu Z, Li G, Wang Q, Ding Y. A proteomic approach to analyze nitrogen and cytokininresponsive proteins in rice roots. Mol Biol Rep, 2012, 39: 1617–1626



[29]Ookawa T, Naruoka Y, Yamazaki T, Suga J, Hirasawa T. A comparison of the accumulation and partitioning of nitrogen in plants between two rice cultivars, Akenohoshi and Nipponbare, at the ripening stage. Plant Prod Sci, 2003, 6: 172–178
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