作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1111-1118.doi: 10.3724/SP.J.1006.2013.01111
陈晓璐1,李耕1,刘鹏1,*,高辉远2,董树亭1,王振林1,张吉旺1,赵斌1
CHEN Xiao-Lu1,LI Geng1,LIU Peng1,*,GAO Hui-Yuan2,DONG Shu-Ting1,WANG Zhen-Lin1,ZHANG Ji-Wang1,ZHAO Bin1
摘要:
以玉米自交系齐319 (Qi 319)为试材,利用叶绿素荧光快速诱导动力学曲线,研究了喷施外源激素6-苄氨基嘌呤(6-BA)对不同氮素水平下玉米花后叶片光系统II (PSII)性能的调控效应。两年研究结果表明,花后穗位叶叶绿素含量和净光合速率(Pn)均呈降低的趋势。在不施氮条件下,外源喷施6-BA,可提高叶绿素含量及后期Pn,但对PSII反应中心的活性无显著改善。施氮可显著提高Pn和PS II的性能,6-BA可显著增强其效果。二者互作条件下,叶绿素含量显著提高,Pn显著增加(P<0.05),PSII供体侧和受体侧的活性得到有效改善,其中对PSII供体侧性能的改善幅度大于对受体侧,PSII反应中心的活性提高以花后10 d最显著。光合性能的提高使单株生物量和籽粒产量显著增加(P<0.05)。因此,在适量施用氮肥的条件下结合喷施6-BA可以显著改善叶片光合性能。
| [1]Dong S-T(董树亭), Gao R-Q(高荣岐), Hu C-H(胡昌浩), Wang Q-Y(王群瑛), Wang K-J(王空军). Study of canopy photosynthesis property and high yield potential after anthesis in maize. Acta Agron Sin (作物学报), 1997, 23(3): 318–325 (in Chinese with English abstract)[2]Simmons S R, Jones R J. Contributions of pre-silking assimilate to grain yield in maize. Crop Sci, 1985, 25: 1004–1006[3]Jin J-Y(金继运), He P(何萍). Effect of N and K nutrition on post metabolism of carbon and nitrogen and grain weight formation in maize. Sci Agric Sin (中国农业科学), 1999, 32(4): 55–62 (in Chinese with English abstract)[4]Shen X-Y(沈秀瑛), Dai J-Y(戴俊英), Hu A-C(胡安畅), Gu W-L(顾慰连), Zhen B(郑波). Studies on relationship among character of canopy light interception and yield in maize populations (Zea mays L.). Acta Agron Sin (作物学报), 1993, 19(3): 246–252 (in Chinese with English abstract)[5]Lawlor D W. Carbon and nitrogen assimilation in relation to yield: mechanisms are the key to understanding production systems. J Exp Bot, 2002, 53: 773–787[6]Cao C-L(曹翠玲), Li S-X(李生秀). Effect of nitrogen level on the photosynthetic rate, NR activity and the contents of nucleic acid of wheat leaf in the stage of reproduction. Chin Bull Bot (植物学通报), 2003, 20(3): 319–324 (in Chinese with English abstract)[7]Yang Q(杨晴), Li Y-M(李雁鸣), Xiao K(肖凯), Du Y-H(杜艳华). Effect of different amount of nitrogen on flag leaf senescence and yield components of wheat. J Hebei Agric Univ (河北农业大学学报), 2002, 25(4): 20–24 (in Chinese with English abstract)[8]Wu X(吴迅), Huang Y-B(黄玉碧), Zheng Z-P(郑祖平), He C(何川), Li Z(李钟), Yang K-X(杨克相), Ren X-J(任兴江), Shi S(史抒). Research development of nitrogen use efficiency in maize. J Heilongjiang Agric Sci (黑龙江农业科学), 2010, (7): 159–161 (in Chinese with English abstract)[9]Wang R-Y(王瑞英), Yu Z-W(于振文), Xu Y-M(许玉敏). Regulation of soaking seeds with combination of IBA and NAA or spraying leaves with 6-BA on senescence of flag leaf and yield in winter wheat. J Shandong Agric Univ (山东农业大学学报), 1998, 29(4): 504–507 (in Chinese with English abstract)[10]Wang Z-M(王志敏), Wang S-A(王树安), Su B-L(苏宝林). Regulation of grain number in wheat. J China Agric Univ (中国农业大学学报), 1994, 1(5): 22–26 (in Chinese with English abstract)[11]Sun Z-Y(孙振元), Han B-W(韩碧文), Liu S-L(刘淑兰), Wang H-F(王华芳), Gao R-F(高荣孚). Absorption and redistribution of nitrogen during grain-filling period of wheat and their regulation by 6-Benzylaminopurine. J Plant Physiol (植物生理学报), 1996, 22(3): 258–264 (in Chinese with English abstract)[12]Wang N-N(王宁宁), Wang Y(王勇), Wang S-F(王淑芳), Zhu L-J(朱亮基), Zhang R(张韧). The relationship between the effect of exogenous 6-BA in retarding soybean leaf senescence and plasma membrane protein phosphorylation. Acta Phytophysiol Sin (植物生理与分子生物学学报), 1998, 24(3): 305–308 (in Chinese with English abstract)[13]Beck E H. Regulation of shoot/root ratio by cytokinins from roots in Urtica dioica: Opinion. Plant Siol, 1996, 185: 1–12[14]Yang L(杨兰), Rong X-M(荣湘民), Song H-X(宋海星), Liu Q(刘强), Zhu Z-K(朱兆坤), Zhang P-C(张鹏程), Gong R(龚蓉). Effects of growth regulator on yield and nitrogen physiological efficiency of rape. J Hunan Agric Sci (湖南农业科学), 2012, (5): 27–29 (in Chinese with English abstract)[15]Haberer G, Kieber J J. Cytokinins: new insights into a classic phytohormone. Plant Physiol, 2002, 128: 354–362[16]Zhao Y-J(赵毓桔). A study on mechanism of 6-BA on delaying the senescence in wheat leaf. J Plant Physiol (植物生理学报), 1978, 4 (1): 39–49 (in Chinese) [17]Garrison F R, Brinker A M, Nooden L D. Relative activities of xylem-supplied cytokinins in retarding soybean leaf senescence and sustaining pod development. Plant Cell Physiol, 1984, 25: 213–224 [18]Dong Y-H(董永华), Shi J-P(史吉平), Li G-M(李广敏). A study on mechanism of 6-BA on increasing of photosynthesis in maize seedlings. J Hebei Agric Sci (河北农业科学), 1996, (1): 1–2 (in Chinese)[19]Shang Z-Q(商振清), Li A-L(李爱丽), Dong Y-H(董永华), Li G-M(李广敏), Han J-M(韩建民), Wang F-R(王凤茹). Effect of 6-BA or KT on photosynthetic capacity in wheat flag leaf and the weight of 1000 grains under water stress. J Hebei Agric Univ (河北农业大学学报), 2000, 23(2): 20–24 (in Chinese with English abstract)[20]Strasser R J, Srivastava A, Tsimilli-Michael M. The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Yunus M, Pathre U, Mohanty P, eds. Probing Photosynthesis: Mechanism, Regulation and Adaptation. London: Taylor & Francis Press, 2000, Chapter 25, pp 445–483[21]Strasser R J, Tsimill-Michael M, Srivastava A. Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou G, Govindjee, eds. Advances in Photosynthesis and Respiration. Netherlands: KAP Press, 2004, Chapter 12, pp 1–47[22]Baker N R. A possible role for photosystem II in environmental perturbations of photosynthesis. Plant Physiol, 1991, 81: 563–570[23]Krause G H, Weis E. Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol, 1991, 42: 313–349[24]Arnon D I. Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris L. Plant Physiol, 1949, 24: 1–15[25]Schansker G, Srivastava A, Govindjee, Strasser R J. Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves. Funct Plant Biol, 2003, 30: 785–796[26]Shan S-M(单守明), Liu G-J(刘国杰), Li S-H(李绍华), Miao P-F(苗鹏飞). Effects of IAA, GA3 and 6-BA applied in autumn on plant quality of strawberry. J Fruit Sci (果树学报), 2007, 24(4): 545–548 (in Chinese with English abstract)[27]Zheng S-S(郑莎莎), Sun C-F(孙传范), Sun H-C(孙红春), Liu L-T(刘连涛), Zhao J-F(赵金峰), Li C-D(李存东). Effects of different exogenous hormones on physiological characteristics of main stem leaves at flower and boll stage in cotton. Sci Agric Sin (中国农业科学), 2009, 42(12): 4383–4389 (in Chinese with English abstract)[28]Tong P-Y(佟屏亚), Cheng Y-N(程延年). Study on economic coefficient of high yield cultivation technology in maize. J Beijing Agric Sci (北京农业科学), 1996, 14(4): 1–3 (in Chinese) [29]Li G(李耕), Gao H-Y(高辉远), Zhao B(赵斌), Dong S-T(董树亭), Zhang J-W(张吉旺), Wang J-F(王敬锋), Liu P(刘鹏). Effects of drought stress on activity of photosystems in leaves of maize at grain filling stage. Acta Agron Sin (作物学报), 2009, 35(10): 1916–1922 (in Chinese with English abstract)[30]Li G(李耕), Zhang S-P(张善平), Liu P(刘鹏), Gao H-Y(高辉远), Wang J-F(王敬锋), Liu C-X(刘晨旭), Dong S-T(董树亭), Zhang J-W(张吉旺). Effect of cadmium on photosystem activities of maize (Zea mays L.) leaves. Sci Agric Sin (中国农业科学), 2011, 44(15): 3118–3126 (in Chinese with English abstract)[31]Li G(李耕), Gao H-Y(高辉远), Liu P(刘鹏), Yang J-S(杨吉顺), Dong S-T(董树亭), Zhang J-W(张吉旺), Wang J-F(王敬锋). Effects of nitrogen fertilization on photosynthetic performance in maize leaf at grain filling stage. Plant Nutr Fert Sin (植物营养与肥料学报), 2010, 16(3): 536–542 (in Chinese with English abstract)[32]Wu C-A(吴长艾), Meng Q-W(孟庆伟), Zou Q(邹琦), Zhao S-J(赵世杰), Wang W(王玮). Comparative study on the photooxidative response in different wheat cultivar leaves. Acta Agron Sin (作物学报), 2003, 29(3): 339–344 (in Chinese with English abstract) [33]Guissé B, Srivastava A, Strasser R J. The polyphasic rise of the chlorophyll a fluorescence (O-K-J-I-P) in heat stressed leaves. Archs Sci Genève, 1995, 48: 147–160[34]Strasser R J, Srivastava A, Govindjee. Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photobiol, 1995, 61: 32–42[35]Chen H X, Li W J, An S Z, Gao H Y. Characterization of PSII photochemistry and thermostability in salt-treated rumex leaves. Plant Physiol, 2004, 161: 257–264[36]Lu C M, Zhang J H. Heat-induced multiple effects on PSII in wheat plants. J Plant Physiol, 2000, 156: 259–265 |
| [1] | 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901. |
| [2] | 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801. |
| [3] | 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308. |
| [4] | 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325. |
| [5] | 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590. |
| [6] | 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352. |
| [7] | 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364. |
| [8] | 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500. |
| [9] | 蔡宏玮, 于爱忠, 姜科强, 王鹏飞, 王玉珑, 霍建喆, 庞小能, 尹波, 尚永盼. 干旱灌区有机肥替代部分化肥促进甜玉米产量提升的关键机制[J]. 作物学报, 2026, 52(4): 1166-1180. |
| [10] | 田红丽, 杨扬, 范亚明, 易红梅, 郭丹丹, 王凤格, 赵久然. 适于玉米品种鉴定的一套三等位变异SNP新型标记组合[J]. 作物学报, 2026, 52(4): 993-1005. |
| [11] | 杨亚莉, 徐明睿, 马越飞, 海艺蕊, 刘凯栋, 刘万茂, 孙颖. 玉米根尖及整根响应缺铁的转录组比较研究[J]. 作物学报, 2026, 52(4): 1006-1021. |
| [12] | 张超, 郭欢, 李忠玲, 岳淑宁, 赵娜. 基于BSA-seq技术定位玉米籽粒花青素关联基因[J]. 作物学报, 2026, 52(3): 780-789. |
| [13] | 于天一, 王春晓, 肖丽, 钟召迪, 王宣仓, 赵勇, 路亚, 吴月, 吴正锋. 不同结瘤特性花生品种氮素累积、产量及品质特性对氮肥用量的响应[J]. 作物学报, 2026, 52(3): 881-894. |
| [14] | 郭向阳, 涂亮, 王栋, 刘鹏飞, 王安贵, 易强, 任洪, 李刚, 祝云芳, 吴迅, 蒋喻林, 田丰, 陈泽辉. 热带Suwan种质在我国玉米种质改良中的创新与利用[J]. 作物学报, 2026, 52(3): 655-664. |
| [15] | 刘宁, 樊平, 王成, 陈琪琪, 成庆悦, 铁夏娜, 汤菁莎, 刘彬彬, 谢鸿堃, 王嘉悦, 施园青, 马均. 减氮配施有机肥对机插稻产量形成与氮素利用的影响[J]. 作物学报, 2026, 52(3): 866-880. |
|
||