作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1111-1118.doi: 10.3724/SP.J.1006.2013.01111

• 耕作栽培·生理生化 • 上一篇    下一篇



  1. 1 山东农业大学农学院 / 作物生物学国家重点实验室,山东泰安271018;2 山东农业大学生命科学学院,山东泰安271018
  • 收稿日期:2012-10-30 修回日期:2013-01-15 出版日期:2013-06-12 网络出版日期:2013-03-22
  • 通讯作者: 刘鹏, E-mail: liupengsdau@126.com, Tel: 0538-8245838, Fax: 0538-8245838
  • 基金资助:

    本研究由国家自然科学基金项目(31071358, 30871476),欧盟FP7国际合作项目(NUE-CROPS 222645),国家“十二五”科技支撑计划项目(2011BAD16B14,2012BAD04B05-2),国家公益性行业(农业)科研专项(HY20121203100, HY1203096),山东省财政支持农业重大应用技术创新课题(2010),山东省良种工程玉米课题(2011)和山东省玉米育种与栽培技术企业重点实验室开放课题(2011)资助。

Effects of Exogenous Hormone 6 Benzyl Adenine (6-BA) on Photosystem II (PSII) Performance of Maize during Process of Leaf Senescence under Different Nitrogen Fertilization Levels after Anthesis

CHEN Xiao-Lu1,LI Geng1,LIU Peng1,*,GAO Hui-Yuan2,DONG Shu-Ting1,WANG Zhen-Lin1,ZHANG Ji-Wang1,ZHAO Bin1   

  1. 1 College of Agronomy, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai’an 271018, China; 2 College of Life Science, Shandong Agricultural University, Tai’an 271018, China
  • Received:2012-10-30 Revised:2013-01-15 Published:2013-06-12 Published online:2013-03-22
  • Contact: 刘鹏, E-mail: liupengsdau@126.com, Tel: 0538-8245838, Fax: 0538-8245838


以玉米自交系齐319 (Qi 319)为试材,利用叶绿素荧光快速诱导动力学曲线,研究了喷施外源激素6-苄氨基嘌呤(6-BA)对不同氮素水平下玉米花后叶片光系统II (PSII)性能的调控效应。两年研究结果表明,花后穗位叶叶绿素含量和净光合速率(Pn)均呈降低的趋势。在不施氮条件下,外源喷施6-BA,可提高叶绿素含量及后期Pn,但对PSII反应中心的活性无显著改善。施氮可显著提高PnPS II的性能,6-BA可显著增强其效果。二者互作条件下,叶绿素含量显著提高,Pn显著增加(P<0.05)PSII供体侧和受体侧的活性得到有效改善,其中对PSII供体侧性能的改善幅度大于对受体侧,PSII反应中心的活性提高以花后10 d最显著。光合性能的提高使单株生物量和籽粒产量显著增加(P<0.05)。因此,在适量施用氮肥的条件下结合喷施6-BA可以显著改善叶片光合性能。

关键词: 玉米, 叶片, 氮素, 6-苄氨基嘌呤(6-BA), 光系统II性能


A two years experiment was conducted using maize inbred line Qi 319 with different nitrogen fertilization levels and exogenous hormone 6 benzyl adenine (6-BA) treatments. The results demonstrated that the chlorophyll content and net photosynthetic rate (Pn) were gradually decreased after anthesis. In treatments of 6-BA without nitrogen fertilizer, chlorophyll contents were increased significantly, but Pn showed no significant increase, and the activity of PSII reaction center was not increased. Under the condition of applying nitrogen fertilizer, Pn and the activity of PSII reaction center were increased significantly, and based on nitrogen fertilizer used, the 6-BA could improve the response of Pn and performance of PSII to nitrogen fertilizer effectively. Under the nitrogen fertilizer and 6-BA interaction conditions, the chlorophyll content and Pn were increased significantly (P<0.05), the activities of donor site and acceptor site of PSII were increased significantly, and the donor site was improved more than that of acceptor site. The most obvious improvement of the activity of PSII reaction center was appeared at the 10th day after anthesis. The improvement of photosynthetic performance significantly improved the single plant dry matter accumulation and grain yield (P<0.05). In conclusion, spraying plant with 6-BA under suitable application of nitrogen fertilizer can significantly improve the photosynthetic performance of maize leaf.

Key words: Maize, Leaf, Nitrogen fertilizer, 6 benzyl adenine (6-BA), Performance of photosystem II

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