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作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1134-1139.doi: 10.3724/SP.J.1006.2013.01134

• 研究简报 • 上一篇    

亚麻遗传连锁图谱的构建

吴建忠,黄文功,康庆华,赵东升,袁红梅,于莹,刘岩,姜卫东,程莉莉,宋喜霞,赵茜,吴广文,关凤芝*   

  1. 黑龙江省农业科学院经济作物研究所, 黑龙江哈尔滨 150086
  • 收稿日期:2012-10-19 修回日期:2013-01-15 出版日期:2013-06-12 网络出版日期:2013-03-22
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-19)资助。

Construction of a Genetic Linkage Map in Flax (Linum usitatissimum L.)

WU Jian-Zhong,HUANG Wen-Gong,KANG Qing-Hua,ZHAO Dong-Sheng,YUAN Hong-Mei,YU Ying,LIU Yan,JIANG Wei-Dong,CHENG Li-Li,SONG Xi-Xia,ZHAO Qian,WU Guang-Wen,GUAN Feng-Zhi*   

  1. Industrial Crops Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2012-10-19 Revised:2013-01-15 Published:2013-06-12 Published online:2013-03-22

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被引次数

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摘要：

利用DIANE (纤用亚麻栽培种)和宁亚17 (油用亚麻栽培种)为杂交亲本，构建30个F2单株作为作图群体，选用71对SRAP和24对SSR共显性标记构建了全长为546.5 cM，含12个连锁群(LGs)的亚麻遗传连锁图谱，标记均匀分布于12个连锁群，每个连锁群有4~15个标记，标记间平均距离为5.75 cM。结果表明，SRAP标记和SSR标记中共显性标记适合于亚麻遗传连锁图谱的构建，但该图谱覆盖的基因组范围较小，需继续图谱的完整性工作。本研究为今后的亚麻在分子生物学方面的研究提供了基础信息。

关键词: 亚麻, SRAP, SSR, 遗传连锁图谱

Abstract:

Using a population with 30 F2 individuals from a cross between DIANE (cultivar for fibre) and NINGYA 17 (cultivar for oil) was established flax genetic linkage map with 71 SRAP primers and 24 SSR primers. The map contained 12 linkage groups which spanned a total map length of 546.5 cM, with four to five marker loci per linkage group. The markers on the map distributed evenly in the 12 linkage groups with an average distance of 5.75 cM between loci. The map merely covered a narrow range of the flax genome and needed further improvement. The results showed that the codominant markers in SRAP and SSR were more suitable for the construction of genetic map in flax. Constructing a integrated genetic linkage map of the whole genome in flax provides important informations for future molecular biology research in flax.

Key words: Flax, SRAP, SSR, Genetic Linkage Map

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