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作物学报

作物学报 ›› 2019, Vol. 45 ›› Issue (9): 1338-1348.doi: 10.3724/SP.J.1006.2019.84172

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用DH和IF2群体检测油菜籽粒油酸、亚油酸、亚麻酸含量QTL

蒙姜宇1,傅鹰1,2,贺亚军1,*(),钱伟1   

  1. 1 西南大学农学与生物科技学院, 重庆 400716
    2 浙江省农业科学院作物与核技术利用研究所, 浙江杭州 310021
  • 收稿日期:2018-12-17 接受日期:2019-05-12 出版日期:2019-09-12 网络出版日期:2019-06-10
  • 通讯作者: 贺亚军
  • 作者简介:E-mail: 1131241810@qq.com
  • 基金资助:
    本研究由国家重点研发计划项目(2016YFD0100202);国家自然科学基金项目(31671729);重庆市基础科学与前沿技术研究项目资助(cstc2017jcyjAX0391)

QTL mapping of oleic acid, linoleic acid and linolenic acid contents in Brassica napus L. using DH and IF2 populations

MENG Jiang-Yu1,FU Ying1,2,HE Ya-Jun1,*(),QIAN Wei1   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
    2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China
  • Received:2018-12-17 Accepted:2019-05-12 Published:2019-09-12 Published online:2019-06-10
  • Contact: Ya-Jun HE
  • Supported by:
    This study was supported by the National Key Research and Development Program of China(2016YFD0100202);the National Natural Science Foundation of China(31671729);Chongqing Research Program of Basic Research and Frontier Technology(cstc2017jcyjAX0391)

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摘要：

以德国冬性甘蓝型油菜Express和中国半冬性甘蓝型油菜SWU07为亲本构建包含261个株系的DH群体和包含234个株系的IF2群体, 检测不同年份条件下油菜籽粒油酸、亚油酸、亚麻酸含量相关的QTL。在DH群体4年环境下共检测出71个QTL, 在IF2群体2年环境下共检测出4个QTL。去掉在不同年份和群体中置信区间相互重叠的QTL之后, 共得到3个品质性状的51个QTL, 其中有15个在2年以上环境中被检测到。这些QTL分别分布在13个连锁群上, 其中与油酸含量相关的18个, 分布于A01、A02、A04、A05、A07、A09、C01连锁群, 揭示3.44%~13.97%的表型变异; 与亚油酸相关的12个, 分布于A02、A06、A09、C01、C02连锁群, 揭示3.84%~19.51%的表型变异; 与亚麻酸相关的21个, 分布于A01、A02、A03、A04、A05、A08、A09、C01、C02、C03、C06连锁群, 揭示2.86%~11.91%的表型变异。这些结果将为油菜脂肪酸品质改良提供更多遗传信息。

关键词: 甘蓝型油菜, 油酸, 亚油酸, 亚麻酸, 数量性状位点

Abstract:

DH and IF2 populations, consisting of 261 and 234 lines respectively, constructed using the German winter rapeseed cultivar ‘Express’ (female) and the Chinese semi-winter line ‘SWU07’ (male), were used to detect QTLs related to oleic acid, linoleic acid and linolenic acid contents in different years. A total of 71 QTLs were detected in DH population in four-year environment and 4 QTLs in IF2 population in two-year environment. After eliminating the overlapped QTLs detected in different years or different populations, totally 51 QTLs for the three traits were obtained, 15 QTLs of which were persistently detected in more than two years. These 51 QTLs were located on 13 linkage groups. Among them, 18 QTLs related to oleic acid content were located on A01, A02, A04, A05, A07, A09, and C01, respectively, explaining phenotypic variation ranging from 3.44% to 13.97%, 12 QTLs related to linoleic acid content were located on A02, A06, A09, C01, and C02, respectively, explaining phenotypic variation ranging from 3.84% to 19.51%, and 21 QTLs related to linolenic acid content were located on A01, A02, A03, A04, A05, A08, A09, C01, C02, C03, and C06, respectively, explaining phenotypic variation from 2.86% to 11.91%. These results provide more genetic information for the improvement of fatty acid quality in rapeseed breeding.

Key words: Brassica napus, oleic acid, linoleic acid, linolenic acid, QTL

表1

亲本油酸、亚油酸、亚麻酸含量的分析"

性状 Trait 油酸 OAC 亚油酸 Llei 亚麻酸 Llen
Express 61.89 19.97 7.70
SWU07 64.67 16.80 6.88
P t-test 0.016 0.018 0.166

表2

DH和IF2群体油酸、亚油酸、亚麻酸含量的分析"

年份
Year		 性状
Trait		 DH群体 DH population IF2群体 IF2 population
最小值
Min.		 最大值
Max.		 平均值
Average		 标准差
SD		 最小值
Min.		 最大值
Max.		 平均值
Average		 标准差
SD
2010 油酸 OAC 49.30 67.10 59.11 3.42 44.83 66.24 59.63 3.12
亚油酸 Llei 14.50 25.30 19.68 2.13 15.77 24.14 19.17 1.82
亚麻酸 Llen 5.44 11.08 8.28 1.06 6.43 11.08 8.36 0.88
2011 油酸 OAC 49.72 77.45 64.60 3.66 59.32 72.53 65.16 2.27
亚油酸 Llei 9.05 23.41 16.33 1.67 13.33 18.87 15.67 1.03
亚麻酸 Llen 4.77 11.40 6.71 0.84 5.21 7.71 6.38 0.54
2012 油酸 OAC 50.94 71.15 60.88 3.34 - - - -
亚油酸 Llei 15.14 25.83 19.81 2.19 - - - -
亚麻酸 Llen 5.19 10.92 8.47 0.97 - - - -
2013 油酸 OAC 52.00 71.37 63.12 3.41 - - - -
亚油酸 Llei 13.41 24.02 18.05 2.15 - - - -
亚麻酸 Llen 5.25 11.28 8.50 1.06 - - - -

图1

DH群体和IF2群体油酸、亚油酸、亚麻酸含量的频率分布"

表3

DH群体和IF2群体同一性状在不同年份间的相关性分析"

年份
Year		 性状
Trait		 DH群体 DH population IF2群体 IF2 population
2010 2011 2012 2013 2010 2011
油酸 OAC 2010
2011 0.4549** 0.3476**
2012 0.5305** 0.5495**
2013 0.4068** 0.5135** 0.5630**
亚油酸 Llei 2010
2011 0.5589** 0.4320**
2012 0.5549** 0.5663**
2013 0.4919** 0.5887** 0.5613**
亚麻酸 Llen 2010
2011 0.4686** 0.2548*
2012 0.5200** 0.4270**
2013 0.5463** 0.4194** 0.6067**

表4

DH 群体和 IF2 群体在同一年份下不同性状间的相关性分析"

年份
Year		 性状
Trait		 油酸
OAC		 亚油酸
Llei		 亚麻酸
Llen
2010 油酸 OAC -0.7018** -0.7200**
亚油酸 Llei -0.6353** 0.6839**
亚麻酸 Llen -0.6510** 0.6791**
2011 油酸 OAC -0.5899** -0.4682**
亚油酸 Llei -0.6439** 0.5080**
亚麻酸 Llen -0.6449** 0.6335**
2012 油酸 OAC
亚油酸 Llei -0.5119**
亚麻酸 Llen -0.6858** 0.5735**
2013 油酸 OAC
亚油酸 Llei -0.6612**
亚麻酸 Llen -0.5695** 0.6210**

表5

DH群体和IF2群体中检测到的与油酸含量相关的QTL"

QTL名称
QTL name		 位置
Position		 加性效应
Additive		 贡献率
R2(%)		 LOD 值
LOD score		 置信区间
Confidence interval
qA01-OAC-2010DH-1 10.31 -0.90 6.83 5.82 9.4-11.1
qA01-OAC-2010DH-2 24.21 -0.87 6.36 5.03 22.6-27.5
qA09-OAC-2010DH-3 48.21 0.79 5.02 4.30 45.3-49.5
qA09-OAC-2010DH-4 53.81 0.68 3.44 2.95 51.9-56.7
qA09-OAC-2010DH-5 76.91 -1.29 12.85 9.41 75.9-81.0
qA09-OAC-2010DH-6 82.41 -1.17 10.84 8.48 81.0-87.7
qA09-OAC-2010DH-7# 91.51 -1.04 8.96 6.33 88.5-98.5
qC01-OAC-2010DH-8# 27.41 -1.38 13.97 10.52 25.4-28.7
qC01-OAC-2010DH-9# 30.71 -1.41 13.72 10.91 29.9-31.0
qA09-OAC-2011DH-10# 94.51 -0.81 4.79 2.65 89.9-98.8
qC01-OAC-2011DH-11# 23.91 -1.09 6.88 4.62 22.6-24.4
qC01-OAC-2011DH-12# 29.31 -1.14 7.91 5.45 28.0-29.9
qA02-OAC-2012DH-13 42.71 0.80 5.52 2.95 41.6-43.4
qA02-OAC-2012DH-14 47.01 0.82 5.70 3.06 44.9-51.0
qA04-OAC-2012DH-15 40.51 -0.91 7.15 3.80 36.4-46.6
qA09-OAC-2012DH-16 70.21 -0.88 5.88 3.42 68.1-70.8
qC01-OAC-2012DH-17 12.71 -0.94 6.30 3.22 12.1-13.7
qC01-OAC-2012DH-18# 23.61 -1.34 11.22 5.33 22.6-23.9
qC01-OAC-2012DH-19# 30.71 -1.21 9.15 4.68 29.3-31.4
qA09-OAC-2013DH-20# 91.51 -0.91 7.02 3.97 89.6-98.8
qC01-OAC-2013DH-21# 25.41 -0.78 4.35 3.00 25.1-28.0
qA02-OAC-2010IF2-22 18.21 -0.91 5.78 2.63 16.2-19.4
qA05-OAC-2011IF2-23 108.51 0.51 4.94 2.50 90.3-115.5
qA07-OAC-2011IF2-24 5.01 0.63 6.42 3.64 1.1-8.1

图2

油酸、亚油酸、亚麻酸含量QTL在连锁群上的分布"

表6

DH群体中检测到的与亚油酸含量相关的QTL"

QTL名称
QTL name		 位置
Position		 加性效应
Additive		 贡献率
R2 (%)		 LOD值
LOD score		 置信区间
Confidence interval
qA09-Llei-2010DH-1 38.21 -0.42 3.84 2.93 34.8-39.5
qA09-Llei-2010DH-2# 68.51 0.77 11.85 8.61 68.1-69.7
qA09-Llei-2010DH-3# 77.81 0.87 15.76 11.77 76.5-81.0
qA09-Llei-2010DH-4# 83.41 0.77 12.24 8.06 81.4-88.5
qC01-Llei-2010DH-5# 26.41 0.74 10.57 7.35 26.0-28.6
qA06-Llei-2011DH-6 38.61 -0.34 4.00 2.83 34.6-41.2
qA09-Llei-2011DH-7# 78.81 0.64 11.61 8.45 77.2-81.2
qA09-Llei-2011DH-8# 94.51 0.48 7.65 4.26 88.5-98.5
qC01-Llei-2011DH-9 23.91 0.50 7.43 5.48 22.6-24.3
qA09-Llei-2012DH-10# 69.71 0.83 13.69 6.52 68.4-72.0
qA09-Llei-2012DH-11# 76.91 0.98 19.51 9.03 75.9-77.8
qA09-Llei-2012DH-12# 96.51 0.72 10.28 4.23 88.5-98.7
qC01-Llei-2012DH-13 6.01 0.55 4.98 2.54 0-12.1
qA02-Llei-2013DH-14 13.21 0.57 5.70 4.16 10.3-18.0
qA09-Llei-2013DH-15# 69.51 0.58 7.23 3.71 68.9-70.8
qA09-Llei-2013DH-16# 77.81 1.05 13.51 9.28 76.6-80.8
qA09-Llei-2013DH-17# 83.41 0.92 12.34 6.68 81.4-88.0
qC01-Llei-2013DH-18 16.11 0.51 4.68 3.31 14.7-17.2
qC01-Llei-2013DH-19# 25.41 0.63 7.33 5.29 25.1-28.0
qC02-Llei-2013DH-20 4.01 -0.51 5.37 3.12 0-8.2

表7

DH群体和IF2群体中检测到与亚麻酸含量相关的QTL"

QTL名称
QTL name		 位置
Position		 加性效应
Additive		 贡献率
R2 (%)		 LOD值
LOD score		 置信区间
Confidence interval
qA02-Llen-2010DH-1 38.11 -0.30 7.53 6.04 37.8-38.6
qA02-Llen-2010DH-2# 44.21 -0.33 9.51 7.88 43.4-44.9
qA08-Llen-2010DH-3 4.01 0.22 3.87 2.82 0-18.8
qA09-Llen-2010DH-4 68.51 0.25 5.60 4.59 68.1-69.7
qA09-Llen-2010DH-5 79.01 0.30 8.05 6.68 76.0-81.2
qC01-Llen-2010DH-6# 23.91 0.41 11.91 9.39 23.3-24.1
qC01-Llen-2010DH-7# 29.91 0.39 10.84 8.84 29.3-31.5
qC03-Llen-2010DH-8 17.61 0.20 2.86 2.62 13.3-20.8
qC06-Llen-2010DH-9 2.01 0.26 5.66 4.32 0-6.6
qC06-Llen-2010DH-10# 7.61 0.23 4.38 3.73 6.6-14.8
qA01-Llen-2011DH-11 63.01 -0.18 3.69 2.63 54.3-69.2
qA03-Llen-2011DH-12# 16.91 0.22 6.77 3.99 15.5-21.1
qA03-Llen-2011DH-13 27.11 0.21 5.95 4.52 25.2-29.0
qA04-Llen-2011DH-14 10.01 -0.16 3.52 2.72 0-20.4
qA05-Llen-2011DH-15 28.81 -0.24 7.92 5.81 25.8-33.1
qA05-Llen-2011DH-16 37.71 -0.18 4.65 3.34 35.5-39.7
qC01-Llen-2011DH-17# 23.91 0.21 4.82 3.61 22.8-24.5
qC01-Llen-2011DH-18 29.31 0.18 3.75 2.85 28.7-30.7
qC03-Llen-2011DH-19 4.01 -0.20 5.43 3.32 0-8.2
qC02-Llen-2011DH-20# 15.21 -0.21 6.32 4.49 9.7-22.8
qC06-Llen-2011DH-21# 7.61 0.17 3.76 2.99 6.6-18.8
qC01-Llen-2012DH-22# 23.61 0.30 6.96 3.23 17.4-23.9
qC01-Llen-2012DH-23# 29.91 0.34 9.16 4.68 28.0-31.1
qC01-Llen-2012DH-24 37.41 0.27 6.92 3.45 36.7-39.2
qC02-Llen-2012DH-25# 12.21 -0.26 6.89 2.73 8.2-14.2
qA02-Llen-2013DH-26# 43.41 -0.27 6.35 4.66 42.2-44.9
qA03-Llen-2013DH-27# 14.91 0.25 5.32 3.48 1.0-20.8
qC01-Llen-2013DH-28# 23.91 0.30 6.48 4.62 22.8-24.4
qC02-Llen-2013DH-29 5.01 -0.34 9.20 4.97 0.9-8.2
qC02-Llen-2013DH-30# 11.21 -0.30 8.07 4.83 8.2-16.9
qC02-Llen-2011IF2-31# 8.21 0.15 5.14 2.93 4.3-13.0
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