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作物学报 ›› 2013, Vol. 39 ›› Issue (07): 1206-1213.doi: 10.3724/SP.J.1006.2013.01206

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

广东不同时期水稻主栽品种抗病基因同源序列多态性分析

唐志明1,*,陈建酉2,陆永法1,周华成1,莫雪娇2,毛兴学2,李晓方3,*   

  1. 1宁波市农业科学研究院作物研究所，浙江宁波 315040; 2广东省农业科学院水稻研究所，广东广州 510640；3长江大学农学院，湖北荆州 434025；
  • 收稿日期:2012-10-22 修回日期:2013-03-19 出版日期:2013-07-12 网络出版日期:2013-04-23
  • 通讯作者: 李晓方, E-mail:lixiaofang@163.com; 唐志明，E-mail: tzm2004@163.com
  • 基金资助:

    本研究由广东省自然科学基金重点项目(06200585)和宁波水稻育种创新团队项目(2009B21001)资助。

Temporal Changes in Resistance Gene Analog Polymorphism of Major Rice Varieties in Guangdong Province

TANG Zhi-Ming1,*,CHEN Jian-You2,LU Yong-Fa1,ZHOU Hua-Cheng1,MO Xue-Jiao2,MAO Xing-Xue2,LI Xiao-Fang3,*   

  1. 1 Crop Research Institute, Ningbo Academy of Agricultural Sciences, Ningbo 315040, China; 2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 3 Agricultural College, Yangtze University, Jingzhou 434025, China
  • Received:2012-10-22 Revised:2013-03-19 Published:2013-07-12 Published online:2013-04-23
  • Contact: 李晓方, E-mail:lixiaofang@163.com; 唐志明，E-mail: tzm2004@163.com

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摘要：

为掌握广东不同时期水稻主栽品种抗病基因同源序列(RGA)多态性变化，利用3对RGA标记分析了广东20世纪50年代初地方品种及1972、1980、1994和2008年主栽品种。按供试品种RGA-PCR指纹聚类，以相似系数0.81为标准将171份品种分为20个类群。这五类品种分别分布于11、7、4、8、11个类群中，它们的Nei遗传多样性指数分别是0.801、0.682、0.493、0.603和0.527。相对于地方品种，1972、1980和1994年品种RGA多态性低是由于品种类群少及品种在各类群中分布均衡性差，2008年品种RGA多态性低是由于该年大多数品种属于同一类群，品种在各类群中分布均衡性差。因此保持品种抗性多样性，既要保证丰富类群，又要确保品种在各类群中分布均衡。近年来品种抗性背景趋同化是广东抗性多样性降低的主要原因，今后在新品种的选育上应均衡利用不同抗源。

关键词: 水稻品种, 抗病基因同源序列, 多态性

Abstract:

In order to investigate the temporal diversity of resistance gene analogs (RGA) from major rice varieties in Guangdong, landraces from early 1950s and major varieties from 1972, 1980, 1994, and 2008 in Guangdong Province were selected and analyzed using three RGA primers. One hundred and seventy-one varieties were divided into twenty groups according to the DNA band data at the similarity index of 0.81. Landraces from early 1950s and varieties form 1972, 1980, 1994, and 2008 were distributed into eleven, seven, four, eight and eleven groups, with their Nei polymorphism index of 0.801, 0.682, 0.493, 0.603, and 0.527, respectively. The RGA polymorphism of landraces was the highest. Having a few group types and unevenly distributing in different groups were the reasons of low diversity for the varieties of 1972, 1980, and 1994, while the low diversity for varieties of 2008 resulted from that major varieties distributed in one group and unevenly distributed among different groups. Thus, having various groups and evenly distribution is necessary for maintaining the resistance diversity of varieties. In recent years, the resistance diversity of rice varieties in Guangdong decreased as major varieties had similar genetic background in resistance. Thus, different resistant resources should be utilized equally in future rice breeding.

Key words: Rice varieties, Resistance gene analog, Polymorphism

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